The compositional analysis of recent cephalopod shell carbohydrates by Fourier transform infrared spectrometry and high performance anion exchange-pulsed amperometric detection

1995 ◽  
Vol 51 (3) ◽  
pp. 278-283 ◽  
Author(s):  
Y. Dauphin ◽  
F. Marin
Keyword(s):  
Fourier Transform ◽  
Anion Exchange ◽  
High Performance ◽  
Compositional Analysis ◽  
Amperometric Detection ◽  
Fourier Transform Infrared ◽  
Infrared Spectrometry ◽  
Fourier Transform Infrared Spectrometry ◽  
Pulsed Amperometric Detection

scholarly journals Feasibility of rapid quantitation of stratum corneum lipid content by Fourier transform infrared spectrometry

2004 ◽  
Vol 18 (3) ◽  
pp. 423-431 ◽  
Author(s):  
Jui-Chen Tsai ◽  
Yu-Li Lo ◽  
Ching-Yu Lin ◽  
Hamm-Ming Sheu ◽  
Jui-Che Lin
Keyword(s):  
Fourier Transform ◽  
Ir Spectra ◽  
Stratum Corneum ◽  
Lipid Content ◽  
High Performance ◽  
Fourier Transform Infrared ◽  
Infrared Spectrometry ◽  
Permeability Barrier ◽  
Fourier Transform Infrared Spectrometry ◽  
Stratum Corneum Lipid

The permeability barrier of skin resides in the stratum corneum, and its properties are mediated by a series of lipid multilayers, enriched in ceramides, cholesterol, and free fatty acids, segregated within the stratum corneum (SC) interstices. SC lipid content is usually determined by gravimetric methods in conjunction with high performance thin layer chromatography, but these methods are time‒consuming and involve hazardous solvents. The objective of the present study was to develop a method of measuring SC lipid content by Fourier transform infrared spectrometry (FTIR) that is fast and requires no solvents. The IR spectra of isolated porcine SC sheets were recorded using a FTIR spectrometer. SC lipid content was determined by gravimetric methods using chloroform–methanol extraction. The peak area of both the CH2symmetric (2850 cm−1) and asymmetric (2920 cm−1) stretching bands in the IR spectra of progressively solvent‒extracted porcine SC sheets decreased with increasing amount of SC lipids removed. When spectral analysis was performed by curve‒fitting using GRAMS/32 software between 3000 to 2800 cm−1, peak area ratios of CH2to CH3asymmetric stretching bands in the IR spectra of 46 isolated porcine SC samples were correlated to SC lipid content (R2=0.90), with the standard error of measurement of 1.91%. The study demonstrated the feasibility of using FTIR technique to rapidly and accurately measure SC lipid content.

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