On the determination of intestinal epithelial cell generation time from labeling index and DNA synthesis duration

1974 ◽  
Vol 30 (2) ◽  
pp. 186-188 ◽  
Author(s):  
J. W. Cooper ◽  
R. F. Hagemann ◽  
G. J. Brodmerkel
Keyword(s):  
Epithelial Cell ◽  
Dna Synthesis ◽  
Generation Time ◽  
Intestinal Epithelial Cell ◽  
Cell Generation ◽  
Labeling Index ◽  
Intestinal Epithelial ◽  
Synthesis Duration

A villous cell-derived inhibitor of intestinal cell proliferation

1981 ◽  
Vol 241 (6) ◽  
pp. G520-G527
Author(s):  
R. J. May ◽  
A. Quaroni ◽  
K. Kirsch ◽  
K. J. Isselbacher
Keyword(s):  
Epithelial Cell ◽  
Dna Synthesis ◽  
Cell Lines ◽  
Intestinal Epithelial Cell ◽  
Amino Acid Uptake ◽  
Intestinal Cell ◽  
Epithelial Cell Line ◽  
Acid Uptake ◽  
Intestinal Epithelial

To test the hypothesis that the intestinal villous cell synthesizes a mitotic inhibitor that is specific for crypt cells, we have partially purified an extract from rat intestinal villous cells (VCE) and have demonstrated that it strongly and reversibly inhibits cell division and DNA synthesis in an intestinal epithelial cell line (IEC-6 cells). VCE produced a 60--70% inhibition of [3H]thymidine incorporation into DNA and a similar magnitude of inhibition of labeling of nuclei in autoradiographic studies. This inhibition was not associated with cytotoxicity as assessed by the effect of VCE on 51Cr release, hexose or amino acid uptake, and protein synthesis. VCE appears specific for IEC-6 cells because it did not affect DNA synthesis in 10 other cell lines, and extracts derived from other cell lines and from colonic mucosa did not affect DNA synthesis in IEC-6 cells. VCE may represent a villous cell factor involved in the control of intestinal epithelial cell turnover in vivo.

Polyamine-modulated expression of c-myc plays a critical role in stimulation of normal intestinal epithelial cell proliferation

2005 ◽  
Vol 288 (1) ◽  
pp. C89-C99 ◽  
Author(s):  
Lan Liu ◽  
Li Li ◽  
Jaladanki N. Rao ◽  
Tongtong Zou ◽  
Huifang M. Zhang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Growth ◽  
Epithelial Cell ◽  
Dna Synthesis ◽  
Intestinal Epithelial Cell ◽  
Small Intestinal Mucosa ◽  
Intestinal Epithelial ◽  
Epithelial Cell Proliferation ◽  
Myc Gene ◽  
Stimulation Of

The nuclear protein c-Myc is a transcription factor involved in the control of cell cycle. Our previous studies indicated that cellular polyamines are absolutely required for cell proliferation in crypts of small intestinal mucosa and that polyamines have the ability to stimulate expression of the c- myc gene. The current study went further to determine whether induced nuclear c-Myc plays a role in stimulation of cell proliferation by polyamines in intestinal crypt cells (IEC-6 line). Exposure of normal quiescent cells after 24-h serum deprivation to 5% dialyzed fetal bovine serum (dFBS) increased both cellular polyamines and expression of the c- myc gene. Increased c-Myc protein formed heterodimers with its binding partner, Max, and specifically bound to the Myc/Max binding site, which was associated with an increase in DNA synthesis. Depletion of cellular polyamines by pretreatment with α-difluoromethylornithine (DFMO) prevented increases in c- myc expression and DNA synthesis induced by 5% dFBS. c- Myc gene transcription and cell proliferation decreased in polyamine-deficient cells, whereas the natural polyamine spermidine given together with DFMO maintained c- myc gene expression and cell growth at normal levels. Disruption of c- myc expression using specific c- myc antisense oligomers not only inhibited normal cell growth (without DFMO) but also prevented the restoration of cell proliferation by spermidine in polyamine-deficient cells. Ectopic expression of wild-type c- myc by recombinant adenoviral vector containing c- myc cDNA increased cell growth. These results indicate that polyamine-induced nuclear c-Myc interacts with Max, binds to the specific DNA sequence, and plays an important role in stimulation of normal intestinal epithelial cell proliferation.

Tu1198 Intestinal Epithelial Cell Extrusion and Microbial Virulence Are Altered in Pediatric Inflammatory Bowel Diseases

2014 ◽  
Vol 146 (5) ◽  
pp. S-781
Author(s):  
Deenaz Zaidi ◽  
Michael Bording-Jorgenson ◽  
Hien Q. Huynh ◽  
Yuefei Lou ◽  
Julia J. Liu ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Inflammatory Bowel Diseases ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
Bowel Diseases ◽  
Inflammatory Bowel ◽  
Cell Extrusion
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