Equivalence of continuous infusion and single injection of3H-thymidine for analysis of intravascular kinetics of neutrophilic granulocytes in the rat

1974 ◽  
Vol 30 (7) ◽  
pp. 816-817 ◽  
Author(s):  
D. Gerecke ◽  
R. Gross
Keyword(s):  
Continuous Infusion ◽  
Single Injection ◽  
Neutrophilic Granulocytes ◽  
Kinetics Of

scholarly journals Studies of the large intestine of sheep

1982 ◽  
Vol 47 (2) ◽  
pp. 301-309 ◽  
Author(s):  
R. M. Dixon ◽  
J. V. Nolan ◽  
L. P. Milligan
Keyword(s):  
Continuous Infusion ◽  
Solid Phase ◽  
Single Injection ◽  
Proximal Colon ◽  
First Order Kinetics ◽  
Tracer Techniques ◽  
Unbiased Estimates ◽  
Brome Grass ◽  
Kinetics Of ◽  
Best Fit

1. Experiments were undertaken to examine the errors associated with the use of indigestible markers, the 51Cr-labelled complex of chromium ethylenediaminetetra-acetic acid (51Cr-EDTA) and 103Ru-labelled tris-(1,10-phenanthroline)-ruthenium(II) chloride (103Ru-P), to measure liquid- and solid-phase digesta kinetics in the caecum and proximal colon of sheep.2. First-order kinetics of markers were observed following either single injection or termination of continuous infusion. There were no differences between the half-times (T½) of marker in the caecum plus proximal colon whether calculated from marker concentration in caecal digesta or in faeces. There were also no differences in the T½ values calculated for the liquid- and solid-phase markers. When pool sizes calculated from the marker kinetics were compared with the volume of digesta present in the caecum and proximal colon at slaughter, it appeared that the 51Cr-EDTA and 103Ru-P caecal pools described the digesta contained in the entire caecum and proximal colon.3. The flow-rates of dry matter (DM) through the caecum of sheep given 694 g lucerne (Medicago sativa) DM/d were similar whether estimated from total collection of faeces, by single injection of marker, or by the ratio, marker concentration: DM in either caecal digesta or faeces during continuous infusion of marker into either the rumen or the caecum.4. In sheep given 553 g brome grass (Bromus inermus) DM/d the coefficient of variation of estimates of the plateau of 51Cr-EDTA marker during continuous infusion into the caecum was greater when 130 ml infusate/d were administered than with 1000 ml/d.5. In the sheep given brome grass the lines of best fit of decline in In 51Cr-EDTA marker concentration v. time following termination of the continuous infusions described previously and following single injection of marker in 20 or 2 ml into the caecum were examined. The variation was least when 1000 ml infusate/d had been administered and was unacceptably large following a single injection of 2 ml.6. These experiments showed that tracer techniques could provide unbiased estimates of trace kinetics in the caecum and proximal colon.

Measurement of leucine metabolism in man from a primed, continuous infusion of L-[1-3C]leucine

1980 ◽  
Vol 238 (5) ◽  
pp. E473-E479 ◽  
Author(s):  
D. E. Matthews ◽  
K. J. Motil ◽  
D. K. Rohrbaugh ◽  
J. F. Burke ◽  
V. R. Young ◽  
...  
Keyword(s):  
Steady State ◽  
Continuous Infusion ◽  
Co2 Production ◽  
Human Beings ◽  
Priming Dose ◽  
Leucine Metabolism ◽  
Kinetics Of ◽  
13Co2 Enrichment

Leucine metabolism in vivo can be determined from a primed, continuous infusion of L-[1-13C]leucine by measuring, at isotopic steady state, plasm [-13C]leucine enrichment, expired 13CO2 enrichment, and CO2 production rate. With an appropriate priming dose of L-[1-13C]leucine and NaH13CO3, isotopic steady state is reached in less than 2 h, and the infusion is completed in 4 h. The method can determine rates of leucine turnover, oxidation, and incorporation into protein with typical relative uncertainties of 2, 10, and 4%, respectively. The method requires no more than 1 ml of blood and uses stable isotope rather than radioisotope techniques. Thus, the method is applicable to studies of human beings of all ages. L-[1-13C]leucine may be infused with a second amino acid labeled with 15N for simultaneous determination of the kinetics of two amino acids.

Maximal secretory capacity of the canine pancreas in response to pancreozymin and secretin

1964 ◽  
Vol 206 (2) ◽  
pp. 351-356 ◽  
Author(s):  
J. Hansky ◽  
O. M. Tiscornia ◽  
D. A. Dreiling ◽  
H. D. Janowitz
Keyword(s):  
Continuous Infusion ◽  
Dose Response ◽  
Single Injection ◽  
Maximal Response ◽  
Maximal Dose ◽  
Response Curves ◽  
Intravenous Injections ◽  
The Mean ◽  
Dog Pancreas ◽  
Secretory Capacity

The output of amylase in nine dogs with chronic pancreatic fistulae has been studied in response to continuous infusions and single intravenous injections of pancreozymin and dose-response curves obtained. A maximal secretory response was reached by each form of administration of the hormone, supramaximal doses giving no further increase. By combining the maximal pancreozymin dose with a maximal secretin dose in a single injection, the maximal amylase, fluid, and bicarbonate secretory capacity of the dog pancreas was determined. On the average, maximal response to pancreozymin was obtained with 0.5–2.0 U/kg min given as a continuous infusion, with 10–25 U/kg as a single injection, and with a dose of 5–15 U/kg when combined with 8 U/kg secretin (maximal dose). In all experiments variability was least at maximal doses. With the combined pancreozymin-secretin injection the mean maximal 15-min output of fluid was 25.7 ml, of amylase 364 U, and of bicarbonate 3.15 mEq.

Kinetics of galactosyl-neoglycoalbumin uptake after continuous infusion into the isolated perfused rat liver

1990 ◽  
Vol 18 (2) ◽  
pp. 292-293 ◽  
Author(s):  
STEPHEN GORE ◽  
ANTHONY I. MORRIS ◽  
IAN T. GILMORE ◽  
DAVID BILLINGTON
Keyword(s):  
Continuous Infusion ◽  
Rat Liver ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Kinetics Of

scholarly journals Gating of IsK expressed in Xenopus oocytes depends on the amount of mRNA injected.

1994 ◽  
Vol 104 (1) ◽  
pp. 87-105 ◽  
Author(s):  
J Cui ◽  
R P Kline ◽  
P Pennefather ◽  
I S Cohen
Keyword(s):  
Xenopus Oocytes ◽  
Single Injection ◽  
Ion Current ◽  
K Channel ◽  
Delayed Rectifier ◽  
Activation Kinetics ◽  
Kinetic Schemes ◽  
Mrna Concentration ◽  
Kinetics Of

IsK is a K+ channel of the delayed rectifier type widely distributed throughout both excitable and nonexcitable cells. Its structure is different from other cloned K+ channels and molecular details of its gating remain obscure. Here we show that the activation kinetics of IsK expressed in Xenopus oocytes depend upon the amount of its mRNA injected, with larger amounts resulting in slower activation kinetics with a longer initial delay during activation. Similar changes in activation kinetics occur with time after a single injection of IsK mRNA. We present two kinetic schemes which illustrate how our experimental results could arise. Both imply an interaction among individual channel proteins during IsK activation. The dependence of channel gating on mRNA concentration provides a novel mechanism for long term regulation of ion current kinetics.

The kinetics of the auto-induction of ifosfamide metabolism during continuous infusion

1995 ◽  
Vol 36 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Alan V. Boddy ◽  
Michael Cole ◽  
Andrew D. J. Pearson ◽  
Jeffrey R. Idle
Keyword(s):  
Continuous Infusion ◽  
Kinetics Of
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