Course of development of isolated rat embryonic ectoderm as renal homograft

1979 ◽  
Vol 35 (2) ◽  
pp. 258-260 ◽  
Author(s):  
B. Levak-Švajger ◽  
A. Švajger
Keyword(s):  
Renal Homograft ◽  
Embryonic Ectoderm ◽  
Isolated Rat

Morphogenetic behaviour of the rat embryonic ectoderm as a renal homograft

Development ◽  
1981 ◽  
Vol 65 (Supplement) ◽  
pp. 243-267
Author(s):  
Anton Švajger ◽  
Božica Levak-Švajger ◽  
Ljiljana Kostović-Kneževic ◽  
Želimir Bradamante
Keyword(s):  
Neural Crest ◽  
Neural Crest Cells ◽  
Primitive Streak ◽  
Mesenchymal Cells ◽  
Epithelial Layer ◽  
Rat Embryo ◽  
Kidney Capsule ◽  
Embryonic Shield ◽  
Renal Homograft ◽  
Embryonic Ectoderm

Halves of transversely or longitudinally cut primary ectoderm of the pre-primitive streak and the early primitive streak rat embryonic shield developed after 15–30 days in renal homografts into benign teratomas composed of various adult tissues, often in perfect organspecific associations. No clear difference exists in histological composition of grafted halves of the same embryonic ectoderm. The primary ectoderm of the pre-primitive streak rat embryonic shield grafted under the kidney capsule for 2 days displayed an atypical morphogenetic behaviour, characterized by diffuse breaking up of the original epithelial layer into mesenchyme. Some of these cells associated into cystic or tubular epithelial structures. The definitive ectoderm of the head-fold-stage rat embryo grown as renal homograft for 1–3 days gave rise to groups of mesenchymal cells. These migrated from the basal side of the ectoderm in a manner which mimicked either the formation of the embryonic mesoderm or the initial migration of neural crest cells. This latter morphogenetic activity was retained in the entire nejjral epithelium of the early somite embryo but was only seen in the caudal open portion of the neural groove at the 10- to 12-somite stage. The efficient histogenesis in grafts of dissected primary ectoderm and the atypical morphogenetic behaviour of grafted primary and definitive rat embryonic ectoderm were discussed in the light of current concepts on mosaic and regulative development, interactive events during embryogenesis and positioning and patterning of cells by controlled morphogenetic cell displacement.

Investigation of inner cell mass determination by aggregation of isolated rat inner cell masses with mouse morulae

Development ◽  
1976 ◽  
Vol 36 (1) ◽  
pp. 163-174
Author(s):  
J. Rossant
Keyword(s):  
Strong Evidence ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Blastocyst Stage ◽  
Mass Determination ◽  
Mouse Uterus ◽  
Immunofluorescent Analysis ◽  
Embryonic Ectoderm ◽  
Isolated Rat

Inner cell masses (ICMs) dissected from 4½-day rat blastocysts were aggregated with 2½-day mouse morulae. Successful aggregates formed blastocysts in vitro and morphologically normal 5½-day conceptuses in the mouse uterus. Immunofluorescent analysis of these conceptuses revealed that rat cells were only present in the embryonic ectoderm and endoderm and never in the trophectoderm derivatives, although rat trophoblast did develop in the mouse uterus in various control experiments. The single-cell resolution of this technique extends the results obtained from aggregating mouse ICMs with mouse morulae and provides strong evidence that ICM cells, although not overtly differentiated, are determined by the blastocyst stage.

Ultrastructural changes in isolated rat liver perfused with cyclic heptapeptide toxins from norwegian freshwater cyanobacteria (blue-green algae)

1987 ◽  
Vol 45 ◽  
pp. 858-859
Author(s):  
A.S. Dabholkar ◽  
W.W. Carmichael ◽  
K. Berg ◽  
J. Wyman
Keyword(s):  
Ultrastructural Changes ◽  
Sprague Dawley ◽  
Isolated Rat Liver ◽  
Blue Green Algae ◽  
Sprague Dawley Rats ◽  
Cyclic Heptapeptide ◽  
Intracellular Changes ◽  
Oscillatoria Agardhii ◽  
Rat Livers ◽  
Isolated Rat

Intracellular changes in the hepatocytes of isolated rat livers perfused with cyclic heptapeptide toxins are described. The toxins used are 1) -Ala-Leu- β-methyl isoAsp-Arg-ADDA-isoGlu-mdha (M.W. 944) from Microcystis aeruginosa- Lake Akersvatn, Norway; 2) -Ala-Arg-isoAsp-Arg-ADDA-isoGlu-mdha (M.W. 1023) from Oscillatoria agardhii var. - Lake Kolbatnvatn, Norway; 3) -Ala-Arg-isoAsp-Arg-ADDA-isoGlu-dha (M.W. 1009) from Oscillatoria agardhii var. isothrix - Lake Froylandsvatn, Norway. Approximate LD intraperitoneal mouse for the toxins is 50, 500 and 1000 μg/kg respectively.Livers were removed from male Sprague Dawley rats and perfused for 15 min with a blood-free perfusate (50 ml) followed by 60 min with perfusate containing i) 25, 50, or 200 μg of M. aeruginosa toxin ii) 50, 250, 500 or 1000 μg of O. agardhii var. toxin and iii) 1000, 2000, 2500 or 5000 μg of O. agardhii var. isothrix toxin. Control livers were perfused for 75 min with the blood-free perfusate.

Effect of Interleukin-8 on histamine release from isolated rat stomach

2001 ◽  
Vol 120 (5) ◽  
pp. A159-A159
Author(s):  
S RO ◽  
K YAKABI ◽  
T NAKAMURA
Keyword(s):  
Histamine Release ◽  
Interleukin 8 ◽  
Rat Stomach ◽  
Isolated Rat

Effect of Ridogrel on Vascular Contractions Gaused by Vasoactive Substances Released during Platelet Activation

1990 ◽  
Vol 64 (01) ◽  
pp. 091-096 ◽  
Author(s):  
W J Janssens ◽  
F J S Cools ◽  
L A M Hoskens ◽  
J M Van Nueten
Keyword(s):  
Smooth Muscle ◽  
Blood Vessel ◽  
Platelet Activation ◽  
Prostaglandin F2α ◽  
Thromboxane A2 ◽  
Femoral Arteries ◽  
Vasoactive Substances ◽  
Caudal Artery ◽  
Isolated Rat ◽  
Rat Platelets

SummaryRidogrel (6.3 × 10−6 to 10−4 M) inhibited contractions of isolated rat caudal arteries and rabbit femoral arteries caused by U-46619. The slope of an Arunlakshana-Schild plot (pA2-value: 3.4 × 10−6 M) on the caudal artery was slightly higher than one (1.14). This effect was maximal within}D min of incubation of the blood vessel with the compound and easily reversible. Ridogrel antagonised contractions of isolated rabbit femoral arteries caused by prostaglandin Fzo2α in the same concentration range. Ridogrel also inhibited contractions induced by aggregating rat platelets on isolated rat caudal arteries (itt the presence of ketanserin 4 × 10−7 M) and on isolated rabbit pulmonary and femoral arteries (in the absence of ketanserin). Ridogrel had no effect on Ca2+-induced contractions in depolarised isolated rabbit femoral arteries, and at 10−4 M antagonised serotonin-induced contractions in this blood vessel. Its effect on serotonin-induced contractions was statistically significant but very small on isolated rat caudal arteries. These observations indicate that ridogrel is an antagonist of prostaglandin endoperoxide/thromboxane A2 and prostaglandin F2α raCeptors on vascular smooth muscle.

Humoral antibodies in renal homograft

JAMA ◽  
1966 ◽  
Vol 198 (3) ◽  
pp. 226-230 ◽  
Author(s):  
F. Milgrom
Keyword(s):  
Humoral Antibodies ◽  
Renal Homograft

Amino acid-induced impairment of glucose oxidation by isolated rat muscle: mechanism of action

2007 ◽  
Vol 115 (S 1) ◽  
Author(s):  
K Stadlbauer ◽  
B Brunmair ◽  
Z Szöcs ◽  
M Krebs ◽  
A Luger ◽  
...  
Keyword(s):  
Amino Acid ◽  
Mechanism Of Action ◽  
Glucose Oxidation ◽  
Rat Muscle ◽  
Isolated Rat
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