The use of cell free extracts derived from fungal protoplasts in the study of aflatoxin biosynthesis

1979 ◽  
Vol 35 (1) ◽  
pp. 21-22 ◽  
Author(s):  
M. S. Anderson ◽  
M. F. Dutton
Keyword(s):  
Aflatoxin Biosynthesis ◽  
Fungal Protoplasts

Carbon catabolite repression gene creA regulates morphology, aflatoxin biosynthesis and virulence in Aspergillus flavus

2018 ◽  
Vol 115 ◽  
pp. 41-51 ◽  
Author(s):  
Opemipo Esther Fasoyin ◽  
Bin Wang ◽  
Mengguang Qiu ◽  
Xiaoyun Han ◽  
Kuang-Ren Chung ◽  
...  
Keyword(s):  
Aspergillus Flavus ◽  
Catabolite Repression ◽  
Carbon Catabolite Repression ◽  
Aflatoxin Biosynthesis

The effect of elevated temperature on gene transcription and aflatoxin biosynthesis

Mycologia ◽  
2007 ◽  
Vol 99 (2) ◽  
pp. 232-239 ◽  
Author(s):  
G.R. OBrian ◽  
D.R. Georgianna ◽  
J.R. Wilkinson ◽  
J. Yu ◽  
H.K. Abbas ◽  
...  
Keyword(s):  
Elevated Temperature ◽  
Gene Transcription ◽  
Aflatoxin Biosynthesis

The effect of elevated temperature on gene transcription and aflatoxin biosynthesis

Mycologia ◽  
2007 ◽  
Vol 99 (2) ◽  
pp. 232-239 ◽  
Author(s):  
G.R. OBrian ◽  
D.R. Georgianna ◽  
J.R. Wilkinson ◽  
J. Yu ◽  
H.K. Abbas ◽  
...  
Keyword(s):  
Elevated Temperature ◽  
Gene Transcription ◽  
Aflatoxin Biosynthesis

Fungal Protoplasts as Genetic Tools

1983 ◽  
pp. 251-251
Author(s):  
J. F. Peberdy ◽  
L. Ferenczy
Keyword(s):  
Genetic Tools ◽  
Fungal Protoplasts

scholarly journals Vibrio gazogenes inhibits aflatoxin production through downregulation of aflatoxin biosynthetic genes in Aspergillus flavus

2022 ◽  
Author(s):  
Shyam L. Kandel ◽  
Rubaiya Jesmin ◽  
Brian M. Mack ◽  
Rajtilak Majumdar ◽  
Matthew K. Gilbert ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Aspergillus Flavus ◽  
Fungal Biomass ◽  
Expression Profiles ◽  
Health Hazard ◽  
Gene Clusters ◽  
Aflatoxin Contamination ◽  
Aflatoxin Biosynthesis ◽  
Control Samples

Aspergillus flavus is an opportunistic pathogen of oilseed crops such as maize, peanut, cottonseed, and tree nuts and produces carcinogenic secondary metabolites known as aflatoxins during seed colonization. Aflatoxin contamination not only reduces the value of the produce but also is a health hazard to humans and animals. Previously, we observed inhibition of A. flavus aflatoxin biosynthesis upon exposure to the marine bacterium, Vibrio gazogenes (Vg). In this study, we used RNA sequencing to examine the transcriptional profiles of A. flavus treated with both live and heat-inactivated dead Vg and control samples. Fungal biomass, total accumulated aflatoxins, and expression profiles of genes constituting secondary metabolite biosynthetic gene clusters were determined at 24, 30, and 40 h after treatment. Statistically significant reductions in total aflatoxins were detected in Vg-treated samples as compared to control samples at 40 h. But no statistical difference in fungal biomass was observed upon these treatments. The Vg treatments were most effective on aflatoxin biosynthesis as was reflected in significant downregulation of majority of the genes in the aflatoxin gene cluster including the aflatoxin pathway regulator gene, aflR. Along with aflatoxin genes, we also observed significant downregulation in some other secondary metabolite gene clusters including cyclopiazonic acid and aflavarin, suggesting that the treatment may inhibit other secondary metabolites as well. Finally, a weighted gene correlation network analysis identified an upregulation of ten genes that were most strongly associated with Vg-dependent aflatoxin inhibition and provide a novel start-point in understanding the mechanisms that result in this phenomenon.

scholarly journals Enzymes and aflatoxin biosynthesis.

1988 ◽  
Vol 52 (2) ◽  
pp. 274-295 ◽  
Author(s):  
M F Dutton
Keyword(s):  
Aflatoxin Biosynthesis

Inhibition of Aflatoxin Biosynthesis by Organophosphorus Compounds

1980 ◽  
Vol 43 (5) ◽  
pp. 381-384 ◽  
Author(s):  
M. F. DUTTON ◽  
M. S. ANDERSON
Keyword(s):  
Aspergillus Flavus ◽  
Organophosphorus Compound ◽  
Organophosphorus Compounds ◽  
Culture Fluid ◽  
Aflatoxin Production ◽  
The Other ◽  
Aflatoxin Biosynthesis ◽  
Inhibition Effect ◽  
Type Activity ◽  
Acetate Derivative

The effect of a range of organophosphorus and various other compounds on production of aflatoxin by Aspergillus flavus was investigated. Five organophosphorus compounds - Chlormephos, Ciodrin, Naled, Phosdrin and Trichlorphon- at concentrations of 20 and 100 μg/ml of culture fluid were found to have activity similar to Dichlorvos, in that they lowered the level of aflatoxin produced and caused formation of several anthraquinone pigments. Two of these pigments have not previously been described, one was named Versicol and a suggested structure is presented, whilst the other compound was shown to be its acetate derivative. A rationale is suggested for the required elements of structure, which are necessary for an organophosphorus compound to have Dichlorvos-type activity. Two unrelated compounds, ammonium nitrate and Tridecanone were also found to elicit Dichlorvos-type activity. It is likely that tridecanone or its breakdown products competitively inhibit enzymes involved in aflatoxin biosynthesis. It is possible that this inhibition effect explains the lowering of aflatoxin production in lipid-rich commodities infected by A. flavus.

scholarly journals The effect of Propolis on inhibition of Aspergillus parasiticus growth, aflatoxin production and expression of aflatoxin biosynthesis pathway genes

2020 ◽  
Vol 18 (1) ◽  
pp. 297-302 ◽  
Author(s):  
Hamideh Mahmoodzadeh Hosseini ◽  
Siavash Hamzeh Pour ◽  
Jafar Amani ◽  
Sima Jabbarzadeh ◽  
Mostafa Hosseinabadi ◽  
...  
Keyword(s):  
Aspergillus Parasiticus ◽  
Aflatoxin Production ◽  
Biosynthesis Pathway ◽  
Aflatoxin Biosynthesis
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