Automated enzymic determination of glycerol in beverages by an immobilized glycerol dehydrogenase nylon-tube reactor

1980 ◽  
Vol 171 (6) ◽  
pp. 449-450 ◽  
Author(s):  
Wilhelm Hinsch ◽  
Andreas Antonijevic ◽  
V. Sundaram
Keyword(s):  
Glycerol Dehydrogenase ◽  
Tube Reactor ◽  
Nylon Tube

Single- and coupled-enzyme nylon tube reactors for routine determination of pyruvate and lactate in serum.

1979 ◽  
Vol 25 (2) ◽  
pp. 285-288 ◽  
Author(s):  
P V Sundaram ◽  
W Hinsch
Keyword(s):  
Lactate Dehydrogenase ◽  
Continuous Flow ◽  
Clinical Laboratory ◽  
Low Cost ◽  
Serum Lactate ◽  
Tube Reactor ◽  
Nylon Tube ◽  
Lactate And Pyruvate ◽  
Routine Clinical Laboratory

Abstract We describe the use of a continuous-flow clinical analyzer with an immobilized coupled-enzyme nylon tube reactor and an immobilized single-enzyme nylon tube reactor for routine estimation of lactate and pyruvate in serum. These reactors are incorporated into the flow system of a modified continuous-flow analyzer (Technicon AutoAnalyzer). Results for serum lactate and pyruvate by this method are compared with those by corresponding methods in which the same enzymes are used in solution, either automatically (pyruvate) or manually (lactate) performed. Routine clinical laboratory determinations with use of the coupled-enzyme system lactate dehydrogenase and alanine aminotransferase, co-immobilized in the nylon tube reactor for estimation of lactate, and lactate dehydrogenase reactors for estimation of pyruvate give reliable and reproducible results with high precision at low cost.

Immobilized-enzyme nylon-tube reactor for routine determination of uric acid in serum.

1978 ◽  
Vol 24 (10) ◽  
pp. 1813-1817 ◽  
Author(s):  
P V Sundaram ◽  
M P Igloi ◽  
R Wassermann ◽  
W Hinsch
Keyword(s):  
Clinical Trials ◽  
Uric Acid ◽  
High Precision ◽  
Lower Cost ◽  
Immobilized Enzyme ◽  
Tube Reactor ◽  
Nylon Tube ◽  
Flow Through

Abstract We report here the preparation of an immobilized-enzyme nylon-tube reactor containing uricase (EC 1.7.3.3) and the assembly of a flow-through system (Technicon AutoAnalyzer II) for the routine determination of uric acid in serum. Results of these uric acid analyses by use of immobilized uricase, in conjunction with peroxidase (EC 1.11.1.7) and aminophenazone-dichlorophenol in solution, are compared with those obtained with the same enzyme in solution by use of the uricase-PAP (peroxidase, 4-aminophenazone, dichlorophenol) method. Clinical trials carried out routinely with the uricase reactor give reliable and reproducible results with high precision at an appreciably lower cost. The reactors are stable to continued or intermittent use for at least three months or for 4000 tests.

Immobilized-enzyme nylon-tube reactors for routine determination of urea and citrulline in serum.

1978 ◽  
Vol 24 (2) ◽  
pp. 234-239 ◽  
Author(s):  
P V Sundaram ◽  
M P Igloi ◽  
R Wassermann ◽  
W Hinsch ◽  
K J Knoke
Keyword(s):  
Solution Method ◽  
Immobilized Enzyme ◽  
Low Cost ◽  
System A ◽  
Tube Reactor ◽  
Nylon Tube ◽  
Diacetyl Monoxime ◽  
Flow Through ◽  
Continued Use

Abstract A continuous-flow clinical analyzer for the routine estimation of urea is described that makes use of an immobilized-enzyme nylon-tube reactor as part of a flow-through system (a Technicon AutoAnalyzer I). Results of blood-urea analyses by use of the immobilized urease are compared with determinations made with the diacetyl monoxime method and the urease solution method. Clinical trials carried out routinely with the immobilized enzyme nylon tube reactor give reliable and reproducible results with high precision and low cost. The reactors are stable to intermittent or continued use for at least four months or for 2000 tests. A method is described in which differential colorimetry is used for determining citrulline in blood and which makes use of the immobilized urease, albeit indirectly.

scholarly journals Preparation of some immobilized linked enzyme systems and their use in the automated determination of disaccharides

1974 ◽  
Vol 137 (1) ◽  
pp. 25-32 ◽  
Author(s):  
D. J. Inman ◽  
W. E. Hornby
Keyword(s):  
Glucose Oxidase ◽  
Enzyme System ◽  
Inside Surface ◽  
Enzyme Systems ◽  
Nylon Tube ◽  
In Series ◽  
Automated Determination

1. Glucose oxidase (EC 1.1.3.4), amyloglucosidase (EC 3.2.1.3), invertase (EC 3.2.1.26) and β-galactosidase (EC 3.2.1.23) were covalently attached via glutaraldehyde to the inside surface of nylon tube. 2. The linked enzyme system, comprising invertase immobilized within a nylon tube acting in series with glucose oxidase immobilized in a similar way, was used for the automated determination of sucrose. 3. The linked enzyme system, comprising β-galactosidase immobilized within a nylon tube acting in series with glucose oxidase immobilized in a similar way, was used for the automated determination of lactose. 4. The linked enzyme system, comprising amyloglucosidase immobilized within a nylon tube acting in series with glucose oxidase immobilized in a similar way, was used for the automated determination of maltose. 5. Mixtures of glucose oxidase and amyloglucosidase were immobilized within the same piece of nylon tube and used for the automated determination of maltose. 6. Mixtures of glucose oxidase and invertase were immobilized within the same piece of nylon tube and used for the automated determination of sucrose.

Routine glucose determination in serum by use of an immobilized glucose dehydrogenase nylon-tube reactor.

1979 ◽  
Vol 25 (8) ◽  
pp. 1436-1439 ◽  
Author(s):  
P V Sundaram ◽  
B Blumenberg ◽  
W Hinsch
Keyword(s):  
Solution Method ◽  
Immobilized Enzyme ◽  
Glucose Dehydrogenase ◽  
Serum Glucose ◽  
Glucose Determination ◽  
Tube Reactor ◽  
Nylon Tube ◽  
The Cost ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Continuous Use

Abstract We report a method for immobilizing glucose dehydrogenase on the inside surface of nylon tubes to produce an immobilized-enzyme nylon-tube reactor. The glucose dehydrogenase reactor is integrated into the flow system of a continuous-flow analyzer to facilitate routine analysis of serum glucose at 50 samples/h. We compared results with those by the reference hexokinase/glucose-6-phosphate dehydrogenase solution method. The coefficient of correlation was r = 0.996. A glucose dehydrogenase reactor made starting with 1 mg (250 U) of enzyme was stable during eight weeks of continuous use, that is, for nearly 3500 tests. This reduced the cost of the assay by at least 50-fold, compared with that for a commercial glucose dehydrogenase test pack method.

A simple colorimetric method for determination of serum triglycerides with lipoprotein lipase and glycerol dehydrogenase

1977 ◽  
Vol 81 (2) ◽  
pp. 125-130 ◽  
Author(s):  
Sugiura Mamoru ◽  
Oikawa Tsutomu ◽  
Hirano Kazuyuki ◽  
Maeda Hidemi ◽  
Yoshimura Hiroko ◽  
...  
Keyword(s):  
Lipoprotein Lipase ◽  
Colorimetric Method ◽  
Glycerol Dehydrogenase ◽  
Serum Triglycerides

Single- and coupled-enzyme nylon-tube reactors for plasma glucose determination with glucose oxidase and aldehyde dehydrogenase.

1980 ◽  
Vol 26 (12) ◽  
pp. 1652-1655 ◽  
Author(s):  
W Hinsch ◽  
A Antonijewić ◽  
P V Sundaram
Keyword(s):  
Glucose Oxidase ◽  
Aldehyde Dehydrogenase ◽  
Plasma Glucose ◽  
Continuous Flow ◽  
Flow System ◽  
Low Cost ◽  
Immobilized Enzymes ◽  
Glucose Determination ◽  
Nylon Tube

Abstract We describe routine methods for determining glucose in plasma with use of aldehyde dehydrogenase or glucose oxidase-aldehyde dehydrogenase immobilized in a nylon tube that is integrated into a continuous-flow system. Although the coupled-enzyme nylon-tube reactors require the presence of a third enzyme, catalase, in solution, the kinetics are not so complicated as to preclude reliable routine determination of glucose at very low cost. Precision is good, and results correlate well with those by the method involving glucose oxidase in solution. More than 3000 tests may be carried out with one reactor. The immobilized enzymes are stable for several months at 4 degrees C when not in use.

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