Auxin effects on tracheal differentiation in root callus tissues in vitro

1966 ◽  
Vol 22 (5) ◽  
pp. 300-300
Author(s):  
A. R. Mehta
Keyword(s):  
Root Callus ◽  
Callus Tissues

scholarly journals Isolation of furanocoumarins from Pastinaca sativa L. callus culture

2014 ◽  
Vol 69 (3) ◽  
pp. 193-195 ◽  
Author(s):  
Halina Ekiert ◽  
Wanda Kisiel
Keyword(s):  
Secondary Metabolites ◽  
Callus Culture ◽  
Spectral Methods ◽  
Solid Medium ◽  
Intact Plant ◽  
Pastinaca Sativa ◽  
First Report ◽  
Callus Tissues ◽  
First Time

Four furanocoumarins: bergapten, xanthotoxin, isopimpinellin (linear furanocoumarins) and sphondin (angular furanocoumarin) were isolated for the first time from callus tissues of <em>Pastinaca sativa</em> L.(<em>Apiaceae</em>) cultured in vitro on solid medium. The compounds were identified using spectral methods. They are well-known secondary metabolites of the intact plant. This is the first report on the isolation of sphondin from in vitro plant cultures.

scholarly journals Qualitative and Quantitative Evaluation of Active Constituents in Callus of Lavandula angustifolia plant in Vitro

2020 ◽  
Vol 17 (2(SI)) ◽  
pp. 0591
Author(s):  
Zainab Salman et al.
Keyword(s):  
Leaf Explants ◽  
Volatile Oil ◽  
Gas Chromatography Mass Spectrometry ◽  
Naphthalene Acetic Acid ◽  
Fresh Weight ◽  
Lavandula Angustifolia ◽  
Qualitative And Quantitative ◽  
Main Components ◽  
Callus Tissues

This study was conducted to describe a protocol for the callus establishing culture of Lavandula angustifolia plant and estimating their content of volatile oil. The quantity of volatile oil callus tissues was compared with that of leaves production. Callus was induced from leaf explants on Murashige and Skoog medium (MS) supplemented with Naphthalene acetic acid (NAA) and Benzyl adenine (BA) in different concentrations. Maximum callus fresh weight was obtained in the combination of 10 mg/L BA and 3 mg/L NAA which reached 18 g after four weeks. The results of this work showed that the  quantity of volatile oil from the highest fresh weight callus was 6 ml compared with quantity of 18g of leaves which gave 0.5 ml. Volatile oil of leaf and callus extracts were analyzed using gas chromatography mass spectrometry method (GC-MS) which showed linoleic acid (56.61%) and oleic acid (57.93%) as main components.

scholarly journals Taxonomic structure of tissue endophytic bacterial microbiome in clonal apple rootstock grown on sod-podzolic soils with variant properties

2021 ◽  
pp. 36-44
Author(s):  
V. V. Bobkova ◽  
S. N. Konovalov ◽  
M. T. Upadyshev
Keyword(s):  
Diversity Indices ◽  
Shannon Index ◽  
Taxonomic Structure ◽  
Apple Rootstock ◽  
Apple Rootstocks ◽  
Bacterial Phyla ◽  
Podzolic Soils ◽  
Bacterial Microbiome ◽  
Callus Tissues

The taxonomic structure of tissue endophytic bacterial microbiome was comparatively studied in microplants (undifferentiated explant callus tissues, passage 25) and 5-year clonal apple rootstocks 57-490 and 54-118 cultured from corresponding tissues (passage 1) on sod-podzolic soils with variant granulometry, chemical, physical and physicochemical properties. Proteobacteria (91.6 %) predominated in vitro tissues among other endophytic bacterial phyla in rootstock 57-490, while Proteobacteria (52.5 %) and Firmicutes (47.4 %) — in rootstock 54-118. The endophytic Firmicutes ratio vs. in vitro tissues decreases (0.7-2.0 %) in roots and more severely (0-0.2 %) in leaves. Endophytic Actinobacteriota are revealed in 11.7 % in roots of the study rootstock in heavy loam soil, whilst in medium loam their ratio drops to 2.74.1 % in roots and 0.1-0.2 % in leaves. The phylogenetic diversity indices estimation for main endophytic bacterial phyla in apple rootstock tissue recovers their essentially lower diversity and evenness in culture endosphere (Shannon index 0.42-1.00) vs. open soil roots (1.34-2.08). The leaves Shannon index is typically low (0.06-0.13) indicating poor diversity and evenness of the main endophytic bacterial phyla.

In vitro Withanolide Production by Withania somnifera L. Cultures

2008 ◽  
Vol 63 (5-6) ◽  
pp. 409-412 ◽  
Author(s):  
Farzana Sabir ◽  
Neelam S. Sangwan ◽  
Narayan D. Chaurasiya ◽  
Laxmi N. Misra ◽  
Rajender S. Sangwan
Keyword(s):  
Cell Suspension ◽  
Withania Somnifera ◽  
Cell Suspension Cultures ◽  
Multiple Shoots ◽  
Suspension Cultures ◽  
Root Cultures ◽  
Withanolide A ◽  
Root Callus ◽  
Pharmacologically Active

In vitro multiple shoots, root, callus and cell suspension cultures of Withania somnifera exhibited the potentiality to produce pharmacologically active withanolides. Multiple shoots cultures exhibited an increase in withanolide A accumulation compared to shoots of the mother plant. In vitro generated root cultures as well as callus and suspension cultures also produced withanolides albeit at lower levels.

Callus and cell suspension culture of Chelidonium majus L

2014 ◽  
Vol 11 (2) ◽  
pp. 150-154
Author(s):  
S Otgonpurev ◽  
Kh Altantsetseg ◽  
N Tsevegsuren
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Basal Medium ◽  
Ms Medium ◽  
Chelidonium Majus ◽  
Root Explants ◽  
Root Callus ◽  
Murashige Skoog

Chelidonium majus L has long history as a being useful for the treatment of many diseases in Asian and European countries. Aim of this study is to cultivate callus and cell suspension culture in vitro using plant phytohormones. The proliferative capacity was tested on shoot and root explants, cultivated on Murashige-Skoog basal medium testing two auxins: 2,4-diclorphenoxiacetic acid (2.4D) and napheteline acetic acid in combination with cytokinine: kinnetine (K). Calluses were developed on MS medium with 0.5 mg/l Kin, 0.5 mg/l IAA from root explants, as well when added with 0.5 mg/l NAA and 0.5 mg/l Kin from shoot explants. More biomass of cell suspension culture of shoot and root callus was accumulated on MS medium added with 0.1mg/l Kin. DOI: http://dx.doi.org/10.5564/mjas.v11i2.238 Mongolian Journal of Agricultural Sciences Vol.11(2) 2013 pp.150-154

In vitro flowering of embryoids derived from mature root callus of ginseng (Panax ginseng)

Nature ◽  
1980 ◽  
Vol 284 (5754) ◽  
pp. 341-342 ◽  
Author(s):  
Wei-chin Chang ◽  
Yue-ie Hsing
Keyword(s):  
Panax Ginseng ◽  
In Vitro Flowering ◽  
Root Callus ◽  
Mature Root
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