The influence of iodine-labelling on the non-specific fixation of immunoglobulins to tissues

1970 ◽  
Vol 26 (6) ◽  
pp. 651-652
Author(s):  
P. C. Frei ◽  
S. Cruchaud ◽  
L. Arnaudo ◽  
A. Vannotti
Keyword(s):  
Iodine Labelling ◽  
Specific Fixation

PD-L1 Immunohistochemistry on Cell Block Preparations From Different Fixatives: A Pilot Study

2019 ◽  
Vol 152 (Supplement_1) ◽  
pp. S95-S95
Author(s):  
Victoria Costa ◽  
David Kim ◽  
Abha Goyal ◽  
Bing He ◽  
Yifang Liu ◽  
...  
Keyword(s):  
Pilot Study ◽  
Personalized Medicine ◽  
Cell Block ◽  
Fixation Methods ◽  
Tonsillar Tissue ◽  
Fixed Cell ◽  
Specific Fixation ◽  
Formalin Fixed

Abstract Objectives Immunohistochemistry especially for biomarkers such as PD-L1 in personalized medicine is increasingly being performed on cell blocks from cytopathology samples. We evaluated the effects of staining cell blocks (CBs) from different cytologic fixatives using four different commercially available PD-L1 antibody clones. Methods PD-L1 immunohistochemistry (IHC) using four different commercially available clones was performed on eight cell blocks processed from two different fixatives and also on eight formalin-fixed tissues. The clones used were 22C3 at 1:50 dilution and 1:100 dilution, SP263, SP142, and E1L3N. The cell block (CB) samples contained cells that strongly express PD-L1 and either fixed in CytoLyt (methanol-based fixative, n = 4) or CytoRich (ethanol-formalin-based fixative, n = 4). Formalin-fixed control tissues (tonsillar tissue, n = 4; stomach, n = 4) were also included. Results Expression for PD-L1 was noted on all the CytoRich fixed cell blocks (n = 4) for all four antibody clones, with the intensity and distribution of expression comparable to the formalin fixed tissues (n = 8). However, consistent absence of expression for PD-L1 was noted on all the CytoLyt fixed cell blocks (n = 4) for all four antibody clones. Conclusion The results of this pilot study demonstrate that PD-L1 IHC on cell blocks is feasible. However, there is a need to validate IHC protocols according to specific fixation methods.

Fragment specific fixation with APTUS wrist system for volar rim fractures of the distal radius: a multicentric study

2021 ◽  
Author(s):  
Marco Biondi ◽  
Andrea Poggetti ◽  
Alessandro Fagetti ◽  
Agostino Di Maro ◽  
Prospero Bigazzi ◽  
...  
Keyword(s):  
Distal Radius ◽  
Multicentric Study ◽  
Specific Fixation

scholarly journals STUDIES ON INFLUENZA VIRUS

1941 ◽  
Vol 73 (5) ◽  
pp. 581-599 ◽  
Author(s):  
Edwin H. Lennette ◽  
Frank L. Horsfall
Keyword(s):  
Influenza Virus ◽  
Chick Embryo ◽  
Influenza A Virus ◽  
Influenza A ◽  
Complement Fixation ◽  
Soluble Antigen ◽  
Swine Virus ◽  
Specific Fixation ◽  
Mouse Lungs

Influenza complement fixation tests designed for use with ferret serum are described. Complement-fixing antigens derived from influenza ferret lungs were unsatisfactory due to their low content of soluble antigen; those prepared from mouse lungs or developing chick embryo membranes proved to be better antigenically and were reliable when the various reagents in the test were properly adjusted to eliminate non-specific fixation of complement. The results of cross complement fixation tests indicated that the soluble antigens of the PR8 and W.S. strains of influenza A virus were closely similar, if not identical. They indicated also that the soluble antigen of swine virus possessed components present in the antigens of the human strains of virus.

Fragment-specific fixation for complex intra-articular fractures of the distal radius: results of a prospective single-centre trial

2012 ◽  
Vol 37 (8) ◽  
pp. 765-771 ◽  
Author(s):  
A. S. Gavaskar ◽  
S. Muthukumar ◽  
N. Chowdary
Keyword(s):  
Distal Radius ◽  
Articular Surface ◽  
Distal Radius Fractures ◽  
First Year ◽  
Functional Evaluation ◽  
Radius Fractures ◽  
Articular Fractures ◽  
Functional Scores ◽  
Radial Length ◽  
Specific Fixation

The goal was to evaluate the efficacy of 2.4 mm column-specific plating for intra-articular distal radius fractures. In total, 105 patients with AO type C distal radius fractures were operated on using the locking distal radius system, Synthes. Follow-up assessments including clinical (wrist and forearm range of motion, grip strength), radiological (articular step, radial length and inclination, volar tilt, and ulnar variance), and functional scores (Disabilities of the Arm, Shoulder, and Hand; Patient Rated Wrist Evaluation) were made at regular intervals until 1 year. Union was obtained in all patients. Articular surface was anatomically reconstructed in 74 patients (70.5%). Clinical and functional evaluation showed significant continuous improvements over the first year. C1 fractures had a better chance of anatomical reduction compared with C2 and C3 fractures. Fracture type, quality of reduction, and presence of degenerative changes did not show a significant effect on functional outcome scores. Column-specific fixation of the distal radius can achieve satisfactory results in complex intra-articular fractures.

Column specific fixation for complex tibial plateau fractures – Midterm prospective study in South - Indian population

Injury ◽  
2020 ◽  
Vol 51 (2) ◽  
pp. 497-504
Author(s):  
V. Selvaraj ◽  
Sathish Devadoss ◽  
S. Jayakumar ◽  
P. Gururagavendra ◽  
A. Devadoss
Keyword(s):  
Prospective Study ◽  
Tibial Plateau ◽  
Indian Population ◽  
Tibial Plateau Fractures ◽  
South Indian Population ◽  
South Indian ◽  
Specific Fixation

Tritium and iodine labelling of a gastrin like hexapeptide

1984 ◽  
Vol 21 (1) ◽  
pp. 15-36 ◽  
Author(s):  
Marie-Hélène Charon ◽  
Paul Pham Van Chuong ◽  
Botond Penke ◽  
Jean-Louis Morgat ◽  
Marcelle Dubrasquet ◽  
...  
Keyword(s):  
Iodine Labelling
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