A comparison of static cytofluorometry and flow cytometry for the estimation of ploidy and DNA replication in human breast cancer

1986 ◽  
Vol 7 (1) ◽  
pp. 15-22 ◽  
Author(s):  
Olle Stål ◽  
Claes Klintenberg ◽  
Gunnar Franzen ◽  
Björn Risberg ◽  
Siw Arvidsson ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Flow Cytometry ◽  
Dna Replication ◽  
Human Breast Cancer ◽  
Human Breast ◽  
Static Cytofluorometry

Relevance of DNA ploidy as a measure of genetic deviation: A comparison of flow cytometry and cytogenetics in 25 cases of human breast cancer

Cytometry ◽  
1988 ◽  
Vol 9 (6) ◽  
pp. 612-618 ◽  
Author(s):  
Y. Remvikos ◽  
M. Gerbault-Seurreau ◽  
P. Vielh ◽  
B. Zafrani ◽  
H. Magdelénat ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Flow Cytometry ◽  
Human Breast Cancer ◽  
Dna Ploidy ◽  
Human Breast

Artelastin is a cytotoxic prenylated flavone that disturbs microtubules and interferes with DNA replication in MCF-7 human breast cancer cells

Life Sciences ◽  
2005 ◽  
Vol 77 (3) ◽  
pp. 293-311 ◽  
Author(s):  
Madalena Pedro ◽  
Maria Manuel Ferreira ◽  
Honorina Cidade ◽  
Anake Kijjoa ◽  
Elsa Bronze-da-Rocha ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Dna Replication ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Mcf 7

Inhibitory Effect of Hydroxysafflor Yellow B on the Proliferation of Human Breast Cancer MCF-7 Cells

2019 ◽  
Vol 14 (2) ◽  
pp. 187-197 ◽  
Author(s):  
Chuanjun Qu ◽  
Weiwei Zhu ◽  
Kaijie Dong ◽  
Zhaohai Pan ◽  
Ying Chen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Flow Cytometry ◽  
Cell Apoptosis ◽  
Human Breast Cancer ◽  
S Phase ◽  
Dependent Manner ◽  
Human Breast ◽  
Protein Levels ◽  
Mcf 7

Background:A recent patent has been issued for hydroxysafflor yellow A (HSYA) as a drug to prevent blood circulation disorders. Hydroxysafflor yellow B (HSYB), an isomer of HSYA with antioxidative effects, has been isolated from the florets of Carthamus tinctorius. The effects of HSYB on the proliferation of cancer cells and its mechanism of action have not been investigated.Objective:The aims of this study were to investigate the anti-cancer effects and the molecular mechanism of HSYB for breast cancer MCF-7 cells.Methods:MTT assays and colony formation assays were used to assess the survival and proliferation of MCF-7 cells, respectively. Hoechst 33258 and flow cytometry were used to measure cell apoptosis and flow cytometry to determine effects on the cell cycle. Western blots were used to measure protein levels.Results:Treatment with HSYB reduced survival and proliferation of human breast cancer MCF-7 cells in a dose-dependent manner. Furthermore, HSYB arrested the MCF-7 cell cycle at the S phase and downregulated cyclin D1, cyclin E, and CDK2. Compared with a control group, HSYB suppressed the protein levels of p-PI3K, PI3K, AKT, and p-AKT in MCF-7 cells. In addition, HSYB decreased the levels of Bcl- 2, increased the levels of Bax, cleaved caspase-3 and caspase-9, and subsequently induced MCF-7 cell apoptosis.Conclusion:These data demonstrate that HSYB arrests the MCF-7 cell cycle at the S phase and induces cell apoptosis. Patent US20170246228 indicates that HSYB can be potentially used for the prevention and treatment of human breast cancer.

scholarly journals Image cytometric DNA analysis in human breast cancer analysis may add prognostic information in diploid cases with low S-Phase fraction by flow cytometry

Cytometry ◽  
1992 ◽  
Vol 13 (6) ◽  
pp. 577-585 ◽  
Author(s):  
Bo Baldetorp ◽  
Mårten Fernö ◽  
Anders Fallenius ◽  
Ghita Fallenius-Vecchi ◽  
Ingrid Idvall ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Flow Cytometry ◽  
Human Breast Cancer ◽  
Dna Analysis ◽  
S Phase ◽  
Phase Fraction ◽  
Human Breast ◽  
Prognostic Information

Relationship of flow cytometry results to clinical and steroid receptor status in human breast cancer

1985 ◽  
Vol 6 (2) ◽  
pp. 113-121 ◽  
Author(s):  
Timothy E. Kute ◽  
Hyman B. Muss ◽  
Marbry Hopkins ◽  
Richard Marshall ◽  
Douglas Case ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Flow Cytometry ◽  
Human Breast Cancer ◽  
Steroid Receptor ◽  
Human Breast ◽  
Receptor Status ◽  
Relationship Of
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