Investigation of acetone-butanol-ethanol (ABE) fermentation by fluorescence

1994 ◽  
Vol 4 (1) ◽  
pp. 3-6
Author(s):  
F. Baut ◽  
M. Fick ◽  
M. L. Viriot ◽  
J. C. André ◽  
M. Donner
Keyword(s):  
Abe Fermentation ◽  
Acetone Butanol Ethanol

scholarly journals Effective continuous acetone–butanol–ethanol production with full utilization of cassava by immobilized symbiotic TSH06

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Zhangnan Lin ◽  
Hongjuan Liu ◽  
Jing Wu ◽  
Petra Patakova ◽  
Barbora Branska ◽  
...  
Keyword(s):  
Large Scale ◽  
Immobilized Cells ◽  
Continuous Fermentation ◽  
Abe Fermentation ◽  
Human Beings ◽  
Butanol Production ◽  
System A ◽  
Glucose System ◽  
Acetone Butanol Ethanol ◽  
Full Utilization

Abstract Background Butanol production by fermentation has recently attracted increasingly more attention because of its mild reaction conditions and environmentally friendly properties. However, traditional feedstocks, such as corn, are food supplies for human beings and are expensive and not suitable for butanol production at a large scale. In this study, acetone, butanol, and ethanol (ABE) fermentation with non-pretreated cassava using a symbiotic TSH06 was investigated. Results In batch fermentation, the butanol concentration of 11.6 g/L was obtained with a productivity of 0.16 g/L/h, which was similar to that obtained from glucose system. A full utilization system of cassava was constructed to improve the fermentation performance, cassava flour was used as the substrate and cassava peel residue was used as the immobilization carrier. ABE fermentation with immobilized cells resulted in total ABE and butanol concentrations of 20 g/L and 13.3 g/L, which were 13.6% and 14.7% higher, respectively, than those of free cells. To further improve the solvent productivity, continuous fermentation was conducted with immobilized cells. In single-stage continuous fermentation, the concentrations of total ABE and butanol reached 9.3 g/L and 6.3 g/L with ABE and butanol productivities of 1.86 g/L/h and 1.26 g/L/h, respectively. In addition, both of the high product concentration and high solvent productivity were achieved in a three-stage continuous fermentation. The ABE productivity and concentration was 1.12 g/L/h and 16.8 g/L, respectively. Conclusions The results indicate that TSH06 could produce solvents from cassava effectively. This study shows that ABE fermentation with cassava as a substrate could be an efficient and economical method of butanol production.

scholarly journals Milling byproducts are an economically viable substrate for butanol production using clostridial ABE fermentation

2020 ◽  
Vol 104 (20) ◽  
pp. 8679-8689
Author(s):  
Nils Thieme ◽  
Johanna C. Panitz ◽  
Claudia Held ◽  
Birgit Lewandowski ◽  
Wolfgang H. Schwarz ◽  
...  
Keyword(s):  
Clostridium Beijerinckii ◽  
Abe Fermentation ◽  
Liquid Fuels ◽  
Butanol Production ◽  
Enzymatic Pretreatment ◽  
Profitability Analysis ◽  
Key Points ◽  
Wide Range ◽  
Wheat Middlings ◽  
Acetone Butanol Ethanol

Abstract Butanol is a platform chemical that is utilized in a wide range of industrial products and is considered a suitable replacement or additive to liquid fuels. So far, it is mainly produced through petrochemical routes. Alternative production routes, for example through biorefinery, are under investigation but are currently not at a market competitive level. Possible alternatives, such as acetone-butanol-ethanol (ABE) fermentation by solventogenic clostridia are not market-ready to this day either, because of their low butanol titer and the high costs of feedstocks. Here, we analyzed wheat middlings and wheat red dog, two wheat milling byproducts available in large quantities, as substrates for clostridial ABE fermentation. We could identify ten strains that exhibited good butanol yields on wheat red dog. Two of the best ABE producing strains, Clostridium beijerinckii NCIMB 8052 and Clostridium diolis DSM 15410, were used to optimize a laboratory-scale fermentation process. In addition, enzymatic pretreatment of both milling byproducts significantly enhanced ABE production rates of the strains C. beijerinckii NCIMB 8052 and C. diolis DSM 15410. Finally, a profitability analysis was performed for small- to mid-scale ABE fermentation plants that utilize enzymatically pretreated wheat red dog as substrate. The estimations show that such a plant could be commercially successful. Key points • Wheat milling byproducts are suitable substrates for clostridial ABE fermentation. • Enzymatic pretreatment of wheat red dog and middlings increases ABE yield. • ABE fermentation plants using wheat red dog as substrate are economically viable.

Acetone-butanol-ethanol (ABE) fermentation using the root hydrolysate after extraction of forskolin from Coleus forskohlii

2016 ◽  
Vol 86 ◽  
pp. 594-601 ◽  
Author(s):  
Shirish M. Harde ◽  
Swati B. Jadhav ◽  
Sandip B. Bankar ◽  
Heikki Ojamo ◽  
Tom Granström ◽  
...  
Keyword(s):  
Abe Fermentation ◽  
Coleus Forskohlii ◽  
Acetone Butanol Ethanol

scholarly journals Integrated, systems metabolic picture of acetone-butanol-ethanol fermentation byClostridium acetobutylicum

2015 ◽  
Vol 112 (27) ◽  
pp. 8505-8510 ◽  
Author(s):  
Chen Liao ◽  
Seung-Oh Seo ◽  
Venhar Celik ◽  
Huaiwei Liu ◽  
Wentao Kong ◽  
...  
Keyword(s):  
Gene Regulation ◽  
Systems Engineering ◽  
Carbon Sources ◽  
Population Level ◽  
Abe Fermentation ◽  
Integrated System ◽  
System Level ◽  
Environmental Cues ◽  
Metabolic Reactions ◽  
Acetone Butanol Ethanol

Microbial metabolism involves complex, system-level processes implemented via the orchestration of metabolic reactions, gene regulation, and environmental cues. One canonical example of such processes is acetone-butanol-ethanol (ABE) fermentation byClostridium acetobutylicum, during which cells convert carbon sources to organic acids that are later reassimilated to produce solvents as a strategy for cellular survival. The complexity and systems nature of the process have been largely underappreciated, rendering challenges in understanding and optimizing solvent production. Here, we present a system-level computational framework for ABE fermentation that combines metabolic reactions, gene regulation, and environmental cues. We developed the framework by decomposing the entire system into three modules, building each module separately, and then assembling them back into an integrated system. During the model construction, a bottom-up approach was used to link molecular events at the single-cell level into the events at the population level. The integrated model was able to successfully reproduce ABE fermentations of the WTC. acetobutylicum(ATCC 824), as well as its mutants, using data obtained from our own experiments and from literature. Furthermore, the model confers successful predictions of the fermentations with various network perturbations across metabolic, genetic, and environmental aspects. From foundation to applications, the framework advances our understanding of complex clostridial metabolism and physiology and also facilitates the development of systems engineering strategies for the production of advanced biofuels.

Butanol production from the effluent of hydrogen fermentation

2011 ◽  
Vol 63 (6) ◽  
pp. 1236-1240 ◽  
Author(s):  
W. H. Chen ◽  
S. Y. Chen ◽  
S. J. Chao ◽  
Z. C. Jian
Keyword(s):  
Consumption Rate ◽  
Clostridium Beijerinckii ◽  
Abe Fermentation ◽  
Acetate Buffer ◽  
Microbial Populations ◽  
Butanol Production ◽  
Practical Application ◽  
Carbohydrate Consumption ◽  
Acetone Butanol Ethanol ◽  
Acetate Butyrate

The purpose of the study was to recover butanol from the effluent of the hydrogen-producing bioreactor containing acetate, butyrate, and carbohydrate. The butanol production by Clostridium beijerinckii NRRL B592 was evaluated under both unsterilized and sterilized conditions for examining the potential of butanol production for the practical application. Sucrose of 10 g/L and butyrate of 2 g/L coupled with acetate buffer were used to mimic the effluent. Sucrose was completely consumed in the both unsterilized and sterilized conditions during acetone-butanol-ethanol (ABE) fermentation. However, the results illustrate that the carbohydrate consumption rate in the unsterilized condition was higher than that in the sterilized condition. The maximum butanol concentrations of 3,500 and 3,750 mg/L were achieved in the sterilized and unsterilized conditions, respectively. Meanwhile, it was found that the acetate and the butyrate concentrations of 600 and 1,500 mg/L, and 300 and 1,000 mg/L were ingested to yield butanol in the sterilized condition and in the unsterilized condition, respectively. The results concluded that high levels of acetate and butyrate could eliminate the interference of other microbial populations, resulting in the enrichment of C. beijerinckii NRRL B592 in the fermentor. The butanol production by C. beijerinckii NRRL B592 could be, therefore, produced from the effluent of the hydrogen-producing bioreactor. It promised that the microbial butanol production is one of attractive bioprocesses to recover energy from wastes.

scholarly journals Effect of lignocellulose-derived weak acids on butanol production byClostridium acetobutylicumunder different pH adjustment conditions

RSC Advances ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 1967-1975 ◽  
Author(s):  
Jianhui Wang ◽  
Hongyan Yang ◽  
Gaoxaing Qi ◽  
Xuecheng Liu ◽  
Xu Gao ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Formic Acid ◽  
Clostridium Acetobutylicum ◽  
Levulinic Acid ◽  
Abe Fermentation ◽  
Butanol Production ◽  
Weak Acids ◽  
Ph Adjustment ◽  
Acetone Butanol Ethanol

The effects of formic acid, acetic acid and levulinic acid on acetone–butanol–ethanol (ABE) fermentation under different pH adjustment conditions were investigated usingClostridium acetobutylicumas the fermentation strain.

Assessment of in situ butanol recovery by vacuum during acetone butanol ethanol (ABE) fermentation

2011 ◽  
Vol 87 (3) ◽  
pp. 334-340 ◽  
Author(s):  
Adriano Pinto Mariano ◽  
Nasib Qureshi ◽  
Rubens Maciel Filho ◽  
Thaddeus Chukwuemeka Ezeji
Keyword(s):  
Abe Fermentation ◽  
Butanol Recovery ◽  
Acetone Butanol Ethanol
Sign in / Sign up

Export Citation Format

Share Document