Characterization of the microsomal epoxide hydrolase of hepatic microsomes of the common dab,Limanda limanda

1996 ◽  
Vol 15 (5) ◽  
pp. 421-430 ◽  
Author(s):  
Anne McCord ◽  
Nicola Dunlop ◽  
Ronald M. Stagg ◽  
John A. Craft
Keyword(s):  
Epoxide Hydrolase ◽  
Microsomal Epoxide Hydrolase ◽  
Hepatic Microsomes ◽  
The Common

scholarly journals Microsomal epoxide hydrolase of rat liver. Purification and characterization of enzyme fractions with different chromatographic characteristics

1986 ◽  
Vol 233 (2) ◽  
pp. 607-611 ◽  
Author(s):  
N J Bulleid ◽  
A B Graham ◽  
J A Craft
Keyword(s):  
Ion Exchange ◽  
Rat Liver ◽  
Epoxide Hydrolase ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Phospholipid Content ◽  
Microsomal Epoxide Hydrolase ◽  
Ionic Detergent ◽  
Inhibited Enzyme

Microsomal epoxide hydrolase was purified from rat liver, and different fractions of the purified enzyme, which varied in their contents of phospholipid, were obtained by ion-exchange chromatography. One fraction (A), which did not bind to CM-cellulose, had a high phospholipid content, and a second fraction (B), which was eluted from CM-cellulose at high ionic strength, had a low phospholipid content. Removal of most of the phospholipid from fraction A altered its chromatographic behaviour. When the delipidated material was re-applied to CM-cellulose, most of the enzyme bound to the cation-exchanger. The specific activities of all the fractions described (with styrene epoxide [(1,2-epoxyethyl)benzene] as substrate) were altered by adding the non-ionic detergent Lubrol PX or phospholipid. Lubrol PX inhibited enzyme activity, and phospholipid reversed this inhibition. The various enzyme fractions isolated appeared to be different forms of the same protein, as judged by their minimum Mr values and immunochemical properties. These results indicate that different fractions of epoxide hydrolase isolated by ion-exchange chromatography probably are not different isoenzyme forms.

scholarly journals Cloning and characterization of a microsomal epoxide hydrolase from Heliothis virescens

2013 ◽  
Vol 43 (3) ◽  
pp. 219-228 ◽  
Author(s):  
Shizuo G. Kamita ◽  
Kohji Yamamoto ◽  
Mary M. Dadala ◽  
Khavong Pha ◽  
Christophe Morisseau ◽  
...  
Keyword(s):  
Heliothis Virescens ◽  
Epoxide Hydrolase ◽  
Microsomal Epoxide Hydrolase

Purification and Characterization of Dog Liver Microsomal Epoxide Hydrolase

1994 ◽  
Vol 115 (5) ◽  
pp. 985-990 ◽  
Author(s):  
Noritaka Ariyoshi ◽  
Mitsuko Tanaka ◽  
Yuji Ishii ◽  
Kazuta Oguri
Keyword(s):  
Epoxide Hydrolase ◽  
Microsomal Epoxide Hydrolase ◽  
Purification And Characterization ◽  
Dog Liver
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