Plant cell immobilization in a dual hollow fiber bioreactor

1989 ◽  
Vol 3 (2) ◽  
pp. 139-144 ◽  
Author(s):  
Dong Jin Kim ◽  
Ho Nam Chang ◽  
Jang Ryol Liu
Keyword(s):  
Plant Cell ◽  
Hollow Fiber ◽  
Cell Immobilization ◽  
Hollow Fiber Bioreactor ◽  
Plant Cell Immobilization

A New Method for Plant Cell Immobilization

Planta Medica ◽  
1992 ◽  
Vol 58 (S 1) ◽  
pp. 610-611
Author(s):  
R. Pospĩsilová ◽  
T. Vanˇk ◽  
M. Hamplová ◽  
T. Macek
Keyword(s):  
Plant Cell ◽  
Cell Immobilization ◽  
New Method ◽  
Plant Cell Immobilization

scholarly journals Homogenous overexpression of the extracellular matrix protein Netrin-1 in a hollow fiber bioreactor

2021 ◽  
Author(s):  
Aniel Moya-Torres ◽  
Monika Gupta ◽  
Fabian Heide ◽  
Natalie Krahn ◽  
Scott Legare ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Hollow Fiber ◽  
Mammalian Cells ◽  
Matrix Protein ◽  
Continuous Production ◽  
Extracellular Matrix Protein ◽  
Close Monitoring ◽  
High Quality ◽  
Biolayer Interferometry ◽  
Hollow Fiber Bioreactor

Abstract The production of recombinant proteins for functional and biophysical studies, especially in the field of structural determination, still represents a challenge as high quality and quantities are needed to adequately perform experiments. This is in part solved by optimizing protein constructs and expression conditions to maximize the yields in regular flask expression systems. Still, work flow and effort can be substantial with no guarantee to obtain improvements. This study presents a combination of workflows that can be used to dramatically increase protein production and improve processing results, specifically for the extracellular matrix protein Netrin-1. This proteoglycan is an axon guidance cue which interacts with various receptors to initiate downstream signaling cascades affecting cell differentiation, proliferation, metabolism, and survival. We were able to produce large glycoprotein quantities in mammalian cells, which were engineered for protein overexpression and secretion into the media using the controlled environment provided by a hollow fiber bioreactor. Close monitoring of the internal bioreactor conditions allowed for stable production over an extended period of time. In addition to this, Netrin-1 concentrations were monitored in expression media through biolayer interferometry which allowed us to increase Netrin-1 media concentrations tenfold over our current flask systems while preserving excellent protein quality and in solution behavior. Our particular combination of genetic engineering, cell culture system, protein purification, and biophysical characterization permitted us to establish an efficient and continuous production of high-quality protein suitable for structural biology studies that can be translated to various biological systems. Key points • Hollow fiber bioreactor produces substantial yields of homogenous Netrin-1 • Biolayer interferometry allows target protein quantitation in expression media • High production yields in the bioreactor do not impair Netrin-1 proteoglycan quality Graphical abstract

Development of a Pharmacological Test System in a Miniaturized Hollow Fiber Bioreactor

1998 ◽  
pp. 259-266 ◽  
Author(s):  
H. Maerz ◽  
R. Buchholz ◽  
F. Emmrich ◽  
L. Pfeiffer ◽  
U. Marx
Keyword(s):  
Hollow Fiber ◽  
Test System ◽  
Hollow Fiber Bioreactor ◽  
Pharmacological Test
Sign in / Sign up

Export Citation Format

Share Document