Cytochrome P-450 mediated O-dealkylation of 7-alkoxycoumarins in liver microsomes from rainbow trout (Salmo gairdneri)

1989 ◽  
Vol 6 (4) ◽  
pp. 199-205 ◽  
Author(s):  
Malin Celander ◽  
Lars Förlin ◽  
Tommy Andersson
Keyword(s):  
Rainbow Trout ◽  
Liver Microsomes ◽  
Salmo Gairdneri ◽  
Cytochrome P 450

Biosynthesis of Lecithin by the CDP-Choline Pathway in Liver Microsomes of Rainbow Trout, Salmo gairdneri

1975 ◽  
Vol 32 (9) ◽  
pp. 1633-1637 ◽  
Author(s):  
B. J. Holub ◽  
K. Nilsson ◽  
J. Piekarski ◽  
S. J. Slinger
Keyword(s):  
Rainbow Trout ◽  
Incubation Medium ◽  
Enzymatic Synthesis ◽  
Environmental Temperature ◽  
Incubation Temperature ◽  
Liver Microsomes ◽  
Salmo Gairdneri ◽  
Optimal Activity

The enzymatic synthesis of lecithin from CDP-choline (14C) was characterized in microsomal preparations from the livers of rainbow trout (Salmo gairdneri). Optimal activity was obtained with the addition of 1,2-digylceride and Mg++ to the incubation medium. There was a 290% stimulation in cholinephosphotransferase activity when the incubation temperature was raised from 15 to 37 C. The results indicate that the CDP-choline pathway is operative in the formation of lecithin in the liver of rainbow trout. The possible function of this reaction in the acclimation offish to environmental temperature is discussed.

IN-VITRO METABOLISM OF 4-ANDROSTENE-3,17-DIONE BY HEPATIC MICROSOMES FROM THE RAINBOW TROUT (SALMO GAIRDNERII): EFFECTS OF HYPOPHYSECTOMY AND OESTRADIOL-17β

1981 ◽  
Vol 90 (1) ◽  
pp. 103-112 ◽  
Author(s):  
TIIU HANSSON ◽  
JAN-ÅKE GUSTAFSSON
Keyword(s):  
Rainbow Trout ◽  
Liver Microsomes ◽  
The Other ◽  
Sham Operation ◽  
Hydroxylase Activity ◽  
Oxidoreductase Activity ◽  
Hepatic Microsomes ◽  
Cytochrome P 450 ◽  
Reductive Metabolism

The metabolism of 4-androstene-3,17-dione by liver microsomes from juvenile rainbow trout, Salmo gairdnerii, was studied in vitro. Hypophysectomy of the fish significantly increased mean hepatic 17-hydroxysteroid oxidoreductase activity when compared with that from sham-operated fish but none of the other enzyme activities investigated were affected. Administration of oestradiol-17β resulted in a significant decrease in mean hepatic 6β-hydroxylase activity and total cytochrome P-450 content but had no effect on the 16-hydroxylation or on the reductive metabolism of androstenedione. The effect of oestradiol-17β on hepatic 6β-hydroxylase activity was as pronounced after hypophysectomy as after sham-operation indicating that these effects of oestradiol-17β are mainly direct and independent of the pituitary gland. The results indicate that hypophysial hormone(s) as well as oestradiol-17β play a role in the regulation of hepatic steroid metabolism in trout.

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