Characterization of Na+ transport in normal human fibroblasts and neoplastic H.Ep.2 cells and the role of inhibitin

1988 ◽  
Vol 106 (3) ◽  
pp. 219-231 ◽  
Author(s):  
Gillian Spurlock ◽  
Kevin Morgan ◽  
M. Afzal Mir
Keyword(s):  
Human Fibroblasts ◽  
Na Transport ◽  
Normal Human

scholarly journals Design, Synthesis, and Structural Characterization of Novel Diazaphenothiazines with 1,2,3-Triazole Substituents as Promising Antiproliferative Agents

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4388 ◽  
Author(s):  
Morak-Młodawska ◽  
Pluta ◽  
Latocha ◽  
Jeleń ◽  
Kuśmierz
Keyword(s):  
Anticancer Activity ◽  
Cell Lines ◽  
Human Fibroblasts ◽  
Human Tumor ◽  
Design Synthesis ◽  
Human Tumor Cell Lines ◽  
Ic50 Values ◽  
Normal Human ◽  
Low Cytotoxicity

A series of novel 1,2,3-triazole-diazphenothiazine hybrids was designed, synthesized, and evaluated for anticancer activity against four selected human tumor cell lines (SNB-19, Caco-2, A549, and MDA-MB231). The majority of the synthesized compounds exhibited significant potent activity against the investigated cell lines. Among them, compounds 1d and 4c showed excellent broad spectrum anticancer activity, with IC50 values ranging from 0.25 to 4.66 μM and 0.25 to 6.25 μM, respectively. The most promising compound 1d, possessing low cytotoxicity against normal human fibroblasts NHFF, was used for gene expression analysis using reverse transcription–quantitative real-time PCR (RT–qPCR). The expression of H3, TP53, CDKN1A, BCL-2, and BAX genes revealed that these compounds inhibited the proliferation in all cells (H3) and activated mitochondrial events of apoptosis (BAX/BCL-2).

scholarly journals Role of p14ARF in Replicative and Induced Senescence of Human Fibroblasts

2001 ◽  
Vol 21 (20) ◽  
pp. 6748-6757 ◽  
Author(s):  
Wenyi Wei ◽  
Ruth M. Hemmer ◽  
John M. Sedivy
Keyword(s):  
Replicative Senescence ◽  
Human Fibroblasts ◽  
Growth Arrest ◽  
Fibroblast Cell ◽  
Cell Types ◽  
Major Pathway ◽  
Telomere Shortening ◽  
Normal Human ◽  
Cell Strains

ABSTRACT Following a proliferative phase of variable duration, most normal somatic cells enter a growth arrest state known as replicative senescence. In addition to telomere shortening, a variety of environmental insults and signaling imbalances can elicit phenotypes closely resembling senescence. We used p53−/− and p21−/− human fibroblast cell strains constructed by gene targeting to investigate the involvement of the Arf-Mdm2-p53-p21 pathway in natural as well as premature senescence states. We propose that in cell types that upregulate p21 during replicative exhaustion, such as normal human fibroblasts, p53, p21, and Rb act sequentially and constitute the major pathway for establishing growth arrest and that the telomere-initiated signal enters this pathway at the level of p53. Our results also revealed a number of significant differences between human and rodent fibroblasts in the regulation of senescence pathways.

scholarly journals Characterization of cytotoxic effect of asbestos on normal human fibroblasts.

1983 ◽  
Vol 51 ◽  
pp. 237-239 ◽  
Author(s):  
M J Chang ◽  
N P Singh ◽  
A Turturro ◽  
R W Hart
Keyword(s):  
Cytotoxic Effect ◽  
Human Fibroblasts ◽  
Normal Human

scholarly journals LONG-TERM ESTABLISHMENT OF A HUMAN PLASMACYTE CELL LINE DERIVED FROM A PATIENT WITH IgD MULTIPLE MYELOMA

1974 ◽  
Vol 140 (2) ◽  
pp. 494-507 ◽  
Author(s):  
M. E. Jobin ◽  
J. L. Fahey ◽  
Z. Price
Keyword(s):  
Multiple Myeloma ◽  
Cell Line ◽  
Human Fibroblasts ◽  
Myeloma Protein ◽  
Culture Viability ◽  
Feeder Layers ◽  
Normal Human ◽  
Stimulating Factor

Cell line LA-49, derived from pleural fluid cells of a patient with IgD multiple myeloma, was established in culture and maintained for more than 1 yr. The D-myeloma protein produced in culture was similar to the serum D-myeloma protein in electrophoretic mobility and in delta- and lambda-chain antigens. The plasma cell tumor culture, LA-49, differed from numerous immunoglobulin-producing B-lymphoblastoid cell lines established in this laboratory in: (a) Morphology (revealing various stages of maturation); (b) type of immunoglobulin produced (IgD vs. IgM, IgG, and/or, rarely, IgA); (c) growth characteristics (requirement of plasmacyte-stimulating factor); and (d) chromosomal features (polyploid vs. pseudodiploid). A growth factor was needed for cell division and maintenance of culture viability. This factor was supplied readily by irradiated feeder layers of normal human fibroblasts or conditional media from fibroblast cultures. Preliminary characterization of this factor revealed it to be a protein with a mol wt of approximately 150,000 daltons.

Adaptive Responses to Low-Dose/Low-Dose-Rate γ Rays in Normal Human Fibroblasts: The Role of Growth Architecture and Oxidative Metabolism

2006 ◽  
Vol 166 (6) ◽  
pp. 849-857 ◽  
Author(s):  
Sonia M. de Toledo ◽  
Nesrin Asaad ◽  
Perumal Venkatachalam ◽  
Ling Li ◽  
Roger W. Howell ◽  
...  
Keyword(s):  
Dose Rate ◽  
Oxidative Metabolism ◽  
Low Dose ◽  
Human Fibroblasts ◽  
Adaptive Responses ◽  
Low Dose Rate ◽  
Normal Human ◽  
Γ Rays

Possible role of a septin, SEPT1, in spreading in squamous cell carcinoma DJM-1 cells

2013 ◽  
Vol 394 (2) ◽  
pp. 281-290 ◽  
Author(s):  
Yoko Mizutani ◽  
Hidenori Ito ◽  
Ikuko Iwamoto ◽  
Rika Morishita ◽  
Hiroyuki Kanoh ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cortical Actin ◽  
Cell Substrate ◽  
Actin Structure ◽  
Normal Human ◽  
Immunohistochemical Analyses

Abstract We performed biochemical, histochemical and cell biological characterization of septins by focusing on SEPT1 in human skin tissues and a squamous cell carcinoma (SCC) cell line DJM-1. In immunoblotting, SEPT1, together with other septins, was detected in normal human epidermis, SCC and DJM-1. In immunohistochemical analyses, SEPT1 was detected diffusely in the cytoplasm of human epidermal cells and eccrine gland epithelial cells, and the protein level was increased in some skin tumors. In DJM-1 cells, SEPT1 together with other members of SEPT2-subgroup, SEPT4 and SEPT5, was enriched in lamellipodia and the localization was dependent on the cortical actin structure. SEPT1 distribution at lamellipodia was also observed in melanoma B16 cells. SEPT9, SEPT11 and SEPT14, in contrast, were localized along with microtubules in DJM-1 cells. In immunoprecipitation assays, SEPT1 and SEPT5 were found to form a complex in DJM-1 cells, whereas SEPT9, SEPT11 and SEPT14 formed a distinct complex with other septins including SEPT7, SEPT8 and SEPT10, in which SEPT5 was not included. When SEPT1 was silenced in DJM-1 cells, cell spreading was inhibited. These results suggest that SEPT1 may participate in cell-cell and/or cell-substrate interaction in DJM-1 and exert its function in a coordinated manner with other septins.

scholarly journals Generation and Characterization of a CRISPR/Cas9-Mediated SNAP29 Knockout in Human Fibroblasts

2021 ◽  
Vol 22 (10) ◽  
pp. 5293
Author(s):  
Marie Christine Martens ◽  
Janin Edelkamp ◽  
Christina Seebode ◽  
Mirijam Schäfer ◽  
Susanne Stählke ◽  
...  
Keyword(s):  
Primary Cilia ◽  
Human Fibroblasts ◽  
Human Cell Line ◽  
Epidermal Differentiation ◽  
Loss Of Function ◽  
Lentiviral Transduction ◽  
Protein Receptor ◽  
Models Comparison

Loss-of-function mutations in the synaptosomal-associated protein 29 (SNAP29) lead to the rare autosomal recessive neurocutaneous cerebral dysgenesis, neuropathy, ichthyosis, and keratoderma (CEDNIK) syndrome. SNAP29 is a soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein. So far, it has been shown to be involved in membrane fusion, epidermal differentiation, formation of primary cilia, and autophagy. Recently, we reported the successful generation of two mouse models for the human CEDNIK syndrome. The aim of this investigation was the generation of a CRISPR/Cas9-mediated SNAP29 knockout (KO) in an immortalized human cell line to further investigate the role of SNAP29 in cellular homeostasis and signaling in humans independently of animal models. Comparison of different methods of delivery for CRISPR/Cas9 plasmids into the cell revealed that lentiviral transduction is more efficient than transfection methods. Here, we reported to the best of our knowledge the first successful generation of a CRISPR/Cas9-mediated SNAP29 KO in immortalized human MRC5Vi fibroblasts (c.169_196delinsTTCGT) via lentiviral transduction.

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