Cellular lithium and transepithelial transport across toad urinary bladder

1982 ◽  
Vol 70 (1) ◽  
pp. 69-88 ◽  
Author(s):  
Pauline M. Hughes ◽  
Anthony D. C. Macknight
Keyword(s):  
Urinary Bladder ◽  
Transepithelial Transport ◽  
Toad Urinary Bladder

Epithelial organization and hormone sensitivity of toad urinary bladder cells in culture

1981 ◽  
Vol 241 (2) ◽  
pp. F129-F138 ◽  
Author(s):  
J. P. Johnson ◽  
R. E. Steele ◽  
F. M. Perkins ◽  
J. B. Wade ◽  
A. S. Preston ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Cell Line ◽  
Cell Lines ◽  
Water Permeability ◽  
Sodium Transport ◽  
Time Course ◽  
Cultured Cells ◽  
Transepithelial Transport ◽  
Seeding Density ◽  
Toad Urinary Bladder

Two continuous cell lines (TB-M and TB-6c) derived from epithelial cells of the toad urinary bladder form epithelia in culture that manifest hormone-sensitive transepithelial transport. Development of transepithelial electrical resistance (R) and transport rate (ISC) are dependent on time and density of cells seeded, but steady-state ISC and R are characteristic for each cell line and independent of seeding density. Some responses of intact toad bladder are preserved in culture, whereas others are altered or absent. Neither cell line responds to vasopressin. Analogues of cAMP increase sodium transport and urea permeability in both cell lines but do not affect water permeability. The intramembrane particle aggregates associated with the vasopressin- and cAMP-induced increase in water permeability of the intact bladder could not be detected in the cell lines. Aldosterone increases sodium transport in both cell lines, and the time course and concentration dependence of the response to aldosterone are similar to those of the intact bladder. The relative effect of a series of steroids on ISC reveals corticosterone to be a more potent mineralocorticoid in cultured cells than in the intact bladder.

Microscopic Analysis of Salt and Water Transport Levels in Toad Urinary Bladder

1971 ◽  
Vol 29 ◽  
pp. 388-389
Author(s):  
D. R. DiBona
Keyword(s):  
Urinary Bladder ◽  
Structure Function ◽  
Water Transport ◽  
Microscopic Analysis ◽  
Transepithelial Transport ◽  
Toad Urinary Bladder ◽  
Microscopic Appearance ◽  
Physiologic State

Several studies correlating a physiologic state with a microscopic appearance have been performed using the urinary bladder of the toad. This tissue is considered ideal for studies of transepithelial transport because of its apparent anatomic simplicity and the ease with which it lends itself to physiologic experimentation. The purpose of this report will be two-fold. First, a brief review of the kinds of studies that have been conducted will be provided; second, some examples of recent studies in structure-function relationships will be discussed.

Role of PKC isozymes in water transport in toad urinary bladder

1991 ◽  
Vol 49 ◽  
pp. 302-303
Author(s):  
A.J. Mia ◽  
L.X. Oakford ◽  
T. Yorio
Keyword(s):  
Urinary Bladder ◽  
Apical Membrane ◽  
Membrane Surface ◽  
Protein A ◽  
Cell Types ◽  
Leukemic Cells ◽  
Toad Urinary Bladder ◽  
Pkc Isozymes ◽  
Antibody Labeling

Protein kinase C (PKC) isozymes, when activated, are translocated to particulate membrane fractions for transport to the apical membrane surface in a variety of cell types. Evidence of PKC translocation was demonstrated in human megakaryoblastic leukemic cells, and in cardiac myocytes and fibroblasts, using FTTC immunofluorescent antibody labeling techniques. Recently, we reported immunogold localizations of PKC subtypes I and II in toad urinary bladder epithelia, following 60 min stimulation with Mezerein (MZ), a PKC activator, or antidiuretic hormone (ADH). Localization of isozyme subtypes I and n was carried out in separate grids using specific monoclonal antibodies with subsequent labeling with 20nm protein A-gold probes. Each PKC subtype was found to be distributed singularly and in discrete isolated patches in the cytosol as well as in the apical membrane domains. To determine if the PKC isozymes co-localized within the cell, a double immunogold labeling technique using single grids was utilized.

OSMOTIC INHIBITION OF THE NATRIFERIC RESPONSE OF THE TOAD URINARY BLADDER TO VASOPRESSIN

1975 ◽  
Vol 67 (1) ◽  
pp. 119-125
Author(s):  
P. J. BENTLEY
Keyword(s):  
Urinary Bladder ◽  
Water Movement ◽  
Short Circuit ◽  
Electrical Potential ◽  
Short Circuit Current ◽  
Toad Bladder ◽  
Toad Urinary Bladder ◽  
Electrical Potential Difference ◽  
Osmotic Gradient

SUMMARY The electrical potential difference and short-circuit current (scc, reflecting active transmural sodium transport) across the toad urinary bladder in vitro was unaffected by the presence of hypo-osmotic solutions bathing the mucosal (urinary) surface, providing that the transmural flow of water was small. Vasopressin increased the scc across the toad bladder (the natriferic response), but this stimulation was considerably reduced in the presence of a hypo-osmotic solution on the mucosal side, conditions under which water transfer across the membrane was also increased. This inhibition of the natriferic response did not depend on the direction of the water movement, for if the osmotic gradient was the opposite way to that which normally occurs, the response to vasopressin was still reduced. The natriferic response to cyclic AMP was also inhibited in the presence of an osmotic gradient. Aldosterone increased the scc and Na+ transport across the toad bladder but this response was not changed when an osmotic gradient was present. The physiological implications of these observations and the possible mechanisms involved are discussed.

Action of steroids on H+ and NH 4 + excretion in the toad urinary bladder

1979 ◽  
Vol 49 (4) ◽  
pp. 297-308 ◽  
Author(s):  
Loy W. Frazier ◽  
N. Y. Zachariah
Keyword(s):  
Urinary Bladder ◽  
Toad Urinary Bladder

scholarly journals Vasopressin-stimulated movement of drugs and uric acid across the toad urinary bladder

1976 ◽  
Vol 9 (1) ◽  
pp. 30-35 ◽  
Author(s):  
Sherman D. Levine ◽  
Nicholas Franki ◽  
Roland Einhorn ◽  
Richard M. Hays
Keyword(s):  
Uric Acid ◽  
Urinary Bladder ◽  
Toad Urinary Bladder

Progesterone inhibition of water permeability in Bufo arenarum oocytes and urinary bladder

1996 ◽  
Vol 270 (5) ◽  
pp. F880-F885 ◽  
Author(s):  
P. Ford ◽  
G. Amodeo ◽  
C. Capurro ◽  
C. Ibarra ◽  
R. Dorr ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Xenopus Laevis ◽  
Water Permeability ◽  
Water Channel ◽  
Water Channels ◽  
Toad Urinary Bladder ◽  
Osmotic Permeability ◽  
Bufo Arenarum ◽  
Mrna Extraction

The ovarian oocytes from Bufo arenarum (BAO) but not those from Xenopus laevis (XLO) would have water channels (WC). We now report that the injection of the mRNA from BAO into the oocytes from XLO increased their water osmotic permeability (Pi) (reduced by 0.3 mM HgCl2 and reversed by 5 mM beta-mercaptoethanol). A 30-min challenge with progesterone induced, 18 h later, a reduction of the mercury-sensitive fraction of Pf in the BAO (but not in XLO). The mRNA from BAO pretreated with progesterone lost its capacity to induce WC in the XLO, but the hormone did not affect the expression of the WC in XLO previously injected with the mRNA from BAO. Pf was also measured in urinary bladders of BAO. Eighteen hours after a challenge with progesterone, a reduction in the hydrosmotic response to oxytocin was observed. Finally, the mRNA from the urinary bladder of BAO was injected into XLO. An increase in Pf was observed. This was not the case if, before the mRNA extraction, the bladders were treated with progesterone. We conclude that the BAO WC share progesterone sensitivity with the oxytocin-regulated water channel present in the toad urinary bladder.

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