Surface hydrophobicity and water transport of the toad urinary bladder: Effects of vasopressin

1988 ◽  
Vol 106 (2) ◽  
pp. 119-122 ◽  
Author(s):  
Elizabeth J. Dial ◽  
James Huang ◽  
Roger G. O'Neil ◽  
Brian A. Hills ◽  
Lenard M. Lichtenberger
Keyword(s):  
Urinary Bladder ◽  
Water Transport ◽  
Surface Hydrophobicity ◽  
Toad Urinary Bladder

Melatonin inhibition of vasopressin-stimulated water transport in the toad urinary bladder

1980 ◽  
Vol 211 (3) ◽  
pp. 407-409 ◽  
Author(s):  
M. Stephen Haswell ◽  
William A. Gern ◽  
Charles L. Ralph
Keyword(s):  
Urinary Bladder ◽  
Water Transport ◽  
Toad Urinary Bladder

Microscopic Analysis of Salt and Water Transport Levels in Toad Urinary Bladder

1971 ◽  
Vol 29 ◽  
pp. 388-389
Author(s):  
D. R. DiBona
Keyword(s):  
Urinary Bladder ◽  
Structure Function ◽  
Water Transport ◽  
Microscopic Analysis ◽  
Transepithelial Transport ◽  
Toad Urinary Bladder ◽  
Microscopic Appearance ◽  
Physiologic State

Several studies correlating a physiologic state with a microscopic appearance have been performed using the urinary bladder of the toad. This tissue is considered ideal for studies of transepithelial transport because of its apparent anatomic simplicity and the ease with which it lends itself to physiologic experimentation. The purpose of this report will be two-fold. First, a brief review of the kinds of studies that have been conducted will be provided; second, some examples of recent studies in structure-function relationships will be discussed.

Effects of Adriamycin on Calcium and Water Transport in Toad Urinary Bladder

1984 ◽  
Vol 435 (1 First Colloqu) ◽  
pp. 530-533
Author(s):  
JING S. CHEN ◽  
YUAN H. KANG ◽  
BETTY L. CHEN
Keyword(s):  
Urinary Bladder ◽  
Water Transport ◽  
Toad Urinary Bladder

Role of PKC isozymes in water transport in toad urinary bladder

1991 ◽  
Vol 49 ◽  
pp. 302-303
Author(s):  
A.J. Mia ◽  
L.X. Oakford ◽  
T. Yorio
Keyword(s):  
Urinary Bladder ◽  
Apical Membrane ◽  
Membrane Surface ◽  
Protein A ◽  
Cell Types ◽  
Leukemic Cells ◽  
Toad Urinary Bladder ◽  
Pkc Isozymes ◽  
Antibody Labeling

Protein kinase C (PKC) isozymes, when activated, are translocated to particulate membrane fractions for transport to the apical membrane surface in a variety of cell types. Evidence of PKC translocation was demonstrated in human megakaryoblastic leukemic cells, and in cardiac myocytes and fibroblasts, using FTTC immunofluorescent antibody labeling techniques. Recently, we reported immunogold localizations of PKC subtypes I and II in toad urinary bladder epithelia, following 60 min stimulation with Mezerein (MZ), a PKC activator, or antidiuretic hormone (ADH). Localization of isozyme subtypes I and n was carried out in separate grids using specific monoclonal antibodies with subsequent labeling with 20nm protein A-gold probes. Each PKC subtype was found to be distributed singularly and in discrete isolated patches in the cytosol as well as in the apical membrane domains. To determine if the PKC isozymes co-localized within the cell, a double immunogold labeling technique using single grids was utilized.

OSMOTIC INHIBITION OF THE NATRIFERIC RESPONSE OF THE TOAD URINARY BLADDER TO VASOPRESSIN

1975 ◽  
Vol 67 (1) ◽  
pp. 119-125
Author(s):  
P. J. BENTLEY
Keyword(s):  
Urinary Bladder ◽  
Water Movement ◽  
Short Circuit ◽  
Electrical Potential ◽  
Short Circuit Current ◽  
Toad Bladder ◽  
Toad Urinary Bladder ◽  
Electrical Potential Difference ◽  
Osmotic Gradient

SUMMARY The electrical potential difference and short-circuit current (scc, reflecting active transmural sodium transport) across the toad urinary bladder in vitro was unaffected by the presence of hypo-osmotic solutions bathing the mucosal (urinary) surface, providing that the transmural flow of water was small. Vasopressin increased the scc across the toad bladder (the natriferic response), but this stimulation was considerably reduced in the presence of a hypo-osmotic solution on the mucosal side, conditions under which water transfer across the membrane was also increased. This inhibition of the natriferic response did not depend on the direction of the water movement, for if the osmotic gradient was the opposite way to that which normally occurs, the response to vasopressin was still reduced. The natriferic response to cyclic AMP was also inhibited in the presence of an osmotic gradient. Aldosterone increased the scc and Na+ transport across the toad bladder but this response was not changed when an osmotic gradient was present. The physiological implications of these observations and the possible mechanisms involved are discussed.

Action of steroids on H+ and NH 4 + excretion in the toad urinary bladder

1979 ◽  
Vol 49 (4) ◽  
pp. 297-308 ◽  
Author(s):  
Loy W. Frazier ◽  
N. Y. Zachariah
Keyword(s):  
Urinary Bladder ◽  
Toad Urinary Bladder
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