Cyanine dye fluorescence used to measure membrane potential changes due to the assembly of complement proteins C5b-9

1985 ◽  
Vol 84 (3) ◽  
pp. 249-258 ◽  
Author(s):  
Therese Wiedmer ◽  
Peter J. Sims
Keyword(s):  
Membrane Potential ◽  
Cyanine Dye ◽  
Complement Proteins ◽  
Dye Fluorescence ◽  
Measure Membrane Potential

Mechanism by which cyanine dyes measure membrane potential in red blood cells and phosphatidylcholine vesicles

Biochemistry ◽  
1974 ◽  
Vol 13 (16) ◽  
pp. 3315-3330 ◽  
Author(s):  
Peter J. Sims ◽  
Alan S. Waggoner ◽  
Chao-Huei Wang ◽  
Joseph F. Hoffman
Keyword(s):  
Membrane Potential ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Cyanine Dyes ◽  
Measure Membrane Potential

The use of a cyanine dye in measuring membrane potential in yeast

1984 ◽  
Vol 231 (1) ◽  
pp. 217-225 ◽  
Author(s):  
Antonio Peña ◽  
Salvador Uribe ◽  
Juan P. Pardo ◽  
Miguel Borbolla
Keyword(s):  
Membrane Potential ◽  
Cyanine Dye

scholarly journals PMN heterogeneity: long-term stability of fluorescent membrane potential responses to the chemoattractant N-formyl-methionyl-leucyl- phenylalanine in healthy adults and correlation with respiratory burst activity

Blood ◽  
1986 ◽  
Vol 68 (3) ◽  
pp. 611-618 ◽  
Author(s):  
MP Fletcher ◽  
BE Seligmann
Keyword(s):  
Membrane Potential ◽  
Respiratory Burst ◽  
Healthy Adults ◽  
Polymorphonuclear Neutrophils ◽  
Cyanine Dye ◽  
Potential Response ◽  
Long Term Stability ◽  
Nbt Reduction ◽  
Wbc Count ◽  
Immature Cells

Abstract Polymorphonuclear neutrophils (PMNs) were isolated from 24 healthy adults 20 to 61 years of age and the proportion of cells that demonstrated depolarization responses to the synthetic chemotaxin N- formyl-methionyl-leucyl-phenylalanine (FMLP) were enumerated using the lipophilic fluorescent cyanine dye 3,3′-di-pentyl-oxacarbocyanine [di-O- C(5)(3)] and flow cytometry. The membrane potential responses were correlated to the cells' respiratory burst capabilities as measured by nitroblue tetrazolium (NBT) reduction and/or superoxide production in response to FMLP; 40.2% +/- 15.1% (mean +/- SD) of cells depolarized to FMLP. The mean SE for duplicate determinations 1 hour apart was 3.8% (range 0.4% to 13.6%, n = 15). There was no correlation between the percentage of depolarizing PMNs and the yield of cells, the percentage of immature cells, or the circulating WBC count. There was no difference in the average age of men (34.9 +/- 9.9 years, n = 11) v women (33.8 +/- 8.5, n = 13) (mean +/- SD) studied, or in the percentage of depolarizing PMNs when men and women were compared (42.2 +/- 10.6% v 43.1 +/- 13.3%, respectively). However, there was a significant increase in the percentage of depolarizing PMNs with increasing age of women (r = .61, P less than .025) but not men (r = .03, P greater than .05). Analysis of variance revealed significantly greater person-to-person variability in the membrane potential response than in day-to-day variability in the same person (P less than .0005). The percentage of depolarizing PMNs in response to FMLP was significantly correlated with the percentage of NBT-positive cells from both purified PMNs and from whole blood (r = .849, P less than .0005, r = .857, P less than .05, respectively), and with the amount of superoxide produced, expressed as a percentage of that amount produced by cytochalasin B (cyto-B)-pretreated cells (r = .565, P less than .01). The data indicate that PMNs from healthy adults demonstrate a heterogeneous membrane potential response to the chemotaxin FMLP that correlates with the cells' oxidative responsiveness and that intersubject differences can be detected. In addition, the proportion of responsive PMNs increases with increasing age in women.

scholarly journals Solvation-Shell Effect on the Cyanine-Dye Fluorescence in Binary Liquid Mixtures

2007 ◽  
Vol 221 (4) ◽  
pp. 537-547 ◽  
Author(s):  
N. Kh. Petrov ◽  
M. N. Gulakov ◽  
M. V. Alfimov ◽  
G. Busse ◽  
S. Techert
Keyword(s):  
Liquid Mixtures ◽  
Solvation Shell ◽  
Binary Liquid Mixtures ◽  
Cyanine Dye ◽  
Shell Effect ◽  
Binary Liquid ◽  
Dye Fluorescence
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