Acetylcholine receptor-controlled ion flux in electroplax membrane vesicles: Identification and characterization of membrane properties that affect ion flux measurements

1981 ◽  
Vol 58 (3) ◽  
pp. 203-211 ◽  
Author(s):  
Peter S. Kim ◽  
George P. Hess
Keyword(s):  
Acetylcholine Receptor ◽  
Membrane Vesicles ◽  
Ion Flux ◽  
Membrane Properties ◽  
Flux Measurements ◽  
Identification And Characterization

scholarly journals Identification and characterization of the α-CA in the outer membrane vesicles produced by Helicobacter pylori

2019 ◽  
Vol 34 (1) ◽  
pp. 189-195 ◽  
Author(s):  
Maurizio Ronci ◽  
Sonia Del Prete ◽  
Valentina Puca ◽  
Simone Carradori ◽  
Vincenzo Carginale ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Outer Membrane ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Identification And Characterization

scholarly journals Identification and characterization of cell-bound membrane vesicles

2017 ◽  
Vol 1859 (5) ◽  
pp. 756-766 ◽  
Author(s):  
Qisheng Tang ◽  
Xiaojun Zhang ◽  
Wendiao Zhang ◽  
Siyuan Zhao ◽  
Yong Chen
Keyword(s):  
Membrane Vesicles ◽  
Identification And Characterization

In Situ and In Vitro Identification and Characterization of Cardiac Ganglion Neurons in the Crab, Carcinus maenas

1999 ◽  
Vol 81 (6) ◽  
pp. 2964-2976 ◽  
Author(s):  
Michelle A. Saver ◽  
Jerrel L. Wilkens ◽  
Naweed I. Syed
Keyword(s):  
Cell Culture ◽  
Carcinus Maenas ◽  
Membrane Properties ◽  
Cardiac Ganglion ◽  
Shore Crabs ◽  
Ganglion Neurons ◽  
Identification And Characterization

In situ and in vitro identification and characterization of cardiac ganglion neurons in the crab, Carcinus maenas. The aim of this study was to investigate the intrinsic membrane properties and hormonal responses of individual central pattern generating neurons in the cardiac ganglion of the shore crab Carcinus maenas. Because the cardiac ganglion in this crustacean species is buried within the heart musculature and is therefore inaccessible for direct morphological and electrophysiological analysis, we developed two novel in vitro preparations. First, to make the ganglion accessible, we established a brief enzymatic treatment procedure that enabled us to isolate the entire cardiac ganglion, in the absence of muscle tissue. Second, a cell culture procedure was developed to isolate individual neurons in vitro. With the use of both isolated ganglionic and neuronal cell culture techniques, this study provides the first direct account of the neuroanatomy of the cardiac ganglion in shore crabs. We demonstrate that cultured neurons not only survived the isolation procedures, but that they also maintained their intrinsic membrane and transmitter response properties, similar to those seen in the intact ganglion. Specifically, we tested the peptides proctolin, crustacean cardioactive peptide, the FLRFamide-related peptide F2, and an amine (serotonin) on both isolated ganglion and in vitro culture neurons. We measured changes in neuronal burst rate, burst amplitude, pacemaker slope, and membrane potential oscillation amplitude in response to the above four hormones. Each hormone either increased neuronal activity in spontaneously bursting neurons, or induced a bursting pattern in quiescent cells. The in vitro cell culture system developed here now provides us with an excellent opportunity to elucidate cellular, synaptic and hormonal mechanisms by which cardiac activity is generated in shore crabs.

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