Solasodine glycosides of Solanum unguiculatum (A.) Rich

1995 ◽  
Vol 17 (6) ◽  
pp. 191-194 ◽  
Author(s):  
Taha M. Sarg ◽  
Karl W. Glombitza ◽  
Samia S. Hafez ◽  
Nawal M. Farrag ◽  
Fawkeya A. Abbas
Keyword(s):  
Solasodine Glycosides

Treatment of Bowenʼs Disease on the Penis with Low Concentration of a Standard Mixture of Solasodine Glycosides and Liquid Nitrogen

2011 ◽  
Vol 37 (6) ◽  
pp. 858-861 ◽  
Author(s):  
LEONARD H. GOLDBERG ◽  
JENNIFER M. LANDAU ◽  
MEGAN N. MOODY ◽  
IRENE J. VERGILIS-KALNER
Keyword(s):  
Liquid Nitrogen ◽  
Standard Mixture ◽  
Low Concentration ◽  
Solasodine Glycosides

Solasodine glycosides and diosgenin from Solanum pinnatum

1972 ◽  
Vol 11 (12) ◽  
pp. 3548-3549 ◽  
Author(s):  
A. Urzúa ◽  
B.K. Cassels
Keyword(s):  
Solasodine Glycosides

scholarly journals Fingerprinting of Natural Product by Eastern Blotting Using Monoclonal Antibodies

2012 ◽  
Vol 2012 ◽  
pp. 1-7
Author(s):  
Hiroyuki Tanaka ◽  
Waraporn Putalun ◽  
Yukihiro Shoyama
Keyword(s):  
Monoclonal Antibodies ◽  
Natural Product ◽  
Cross Reactivity ◽  
Blue Color ◽  
Ginsenoside Rb1 ◽  
Double Staining ◽  
Eastern Blotting ◽  
The Central Nervous System ◽  
Rf Values ◽  
Solasodine Glycosides

We succeeded in developing the fingerprint of natural product by eastern blotting using monoclonal antibodies. After developing and separating them on a TLC plate, solasodine glycosides are oxidized by NaIO4 and reacted with a protein to give conjugates which are recognized with anti-solamargine monoclonal antibody (MAb). Anti-solamargine MAb having wide cross-reactivity can stain and detect all solasodine glycosides by fingerprint. Different sensitivity between solamargine and solasonine was observed. The detection limit was 1.6 ng of solasonine. The hydrolysed products of solamargine were determined by fingerprint of eastern blotting compared to their Rf values depending on the sugar number. Fingerprint by eastern blotting using anti-ginsenoside Rb1 MAb distinguished the formula containing ginseng prescribed in traditional Chinese medicine. By double-staining of ginsenosides it is possible to suggest that the staining color shows the pharmacological activity, such as the purple bands indicate ginsenosides having stimulation activity, and the blue color indicated compound like ginsenosides possessed the depression affect for the central nervous system (CNS), respectively.

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