Identification of anion and cation pathways in the inner mitochondrial membrane by patch clamping of mouse liver mitoplasts

Spaceflight induced mitochondrial alterations have been reported for muscle and may be associated with altered physiological functions in space. Mitochondrial alterations are also indicative of preapoptotic events which are seen in greater amounts in cells exposed to spaceflight when compared with cells cultured at 1 g. Preapoptotic mitochondrial changes include alterations of processes at the inner mitochondrial membrane and can result in changes in mitochondrial volume. Higher amounts of oxidative stress during space flight may be one of the causes for changes which lead to apoptosis. Jurkat cells flown on the STS-76 space shuttle mission showed an increase in the number of cells with apoptotic bodies early in the mission and a time-dependent, microgravity-related increase in the Fas/APO-1 cell death factor. Here we investigated the morphology of mitochondria in Jurkat cells exposed to spaceflight during the STS-76 mission.

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Selective labeling and rotational diffusion of the ADP/ATP translocator in the inner mitochondrial membrane.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)68157-3 ◽

1982 ◽

Vol 257 (3) ◽

pp. 1117-1120 ◽

Cited By ~ 3

Author(s):

M. Müller ◽

J.J. Krebs ◽

R.J. Cherry ◽

S. Kawato

Keyword(s):

Mitochondrial Membrane ◽

Rotational Diffusion ◽

Inner Mitochondrial Membrane ◽

Selective Labeling

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Outer and Inner Mitochondrial Membrane Proteins TOMM40 and TIMM50 are Intensively Concentrated and Localized at Purkinje and Pyramidal Neurons in the New Zealand White Rabbit Brain

The Anatomical Record ◽

10.1002/ar.24689 ◽

2021 ◽

Author(s):

Sameh M. Farouk ◽

Ahmed M. Abdellatif ◽

Elsayed Metwally

Keyword(s):

New Zealand ◽

Membrane Proteins ◽

Mitochondrial Membrane ◽

Pyramidal Neurons ◽

Zealand White Rabbit ◽

Rabbit Brain ◽

Inner Mitochondrial Membrane ◽

New Zealand White Rabbit

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Mitochondrial Fusion in Yeast Requires the Transmembrane GTPase Fzo1p

The Journal of Cell Biology ◽

10.1083/jcb.143.2.359 ◽

1998 ◽

Vol 143 (2) ◽

pp. 359-373 ◽

Cited By ~ 376

Author(s):

Greg J. Hermann ◽

John W. Thatcher ◽

John P. Mills ◽

Karen G. Hales ◽

Margaret T. Fuller ◽

...

Keyword(s):

Mitochondrial Membrane ◽

Fusion Reaction ◽

Point Mutations ◽

Integral Membrane Protein ◽

Mitochondrial Fusion ◽

Inner Mitochondrial Membrane ◽

Fusion Event ◽

Gtpase Domain ◽

Developmentally Regulated ◽

Mitochondrial Reticulum

Membrane fusion is required to establish the morphology and cellular distribution of the mitochondrial compartment. In Drosophila, mutations in the fuzzy onions (fzo) GTPase block a developmentally regulated mitochondrial fusion event during spermatogenesis. Here we report that the yeast orthologue of fuzzy onions, Fzo1p, plays a direct and conserved role in mitochondrial fusion. A conditional fzo1 mutation causes the mitochondrial reticulum to fragment and blocks mitochondrial fusion during yeast mating. Fzo1p is a mitochondrial integral membrane protein with its GTPase domain exposed to the cytoplasm. Point mutations that alter conserved residues in the GTPase domain do not affect Fzo1p localization but disrupt mitochondrial fusion. Suborganellar fractionation suggests that Fzo1p spans the outer and is tightly associated with the inner mitochondrial membrane. This topology may be required to coordinate the behavior of the two mitochondrial membranes during the fusion reaction. We propose that the fuzzy onions family of transmembrane GTPases act as molecular switches to regulate a key step in mitochondrial membrane docking and/or fusion.

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