Dimensions of polar pathways through rabbit gallbladder epithelium

1974 ◽  
Vol 15 (1) ◽  
pp. 363-382 ◽  
Author(s):  
C. H. van Os ◽  
M. D. de Jong ◽  
J. F. G. Slegers
Keyword(s):  
Gallbladder Epithelium ◽  
Rabbit Gallbladder

Localization of Sodium in Normal and Inhibited Transporting Epithelia

1971 ◽  
Vol 29 ◽  
pp. 392-393
Author(s):  
G. I. Kaye ◽  
J. D. Cole
Keyword(s):  
Plasma Membrane ◽  
Similar Pattern ◽  
Fixation Method ◽  
Colonic Epithelium ◽  
Gallbladder Epithelium ◽  
Rabbit Colon ◽  
Rabbit Gallbladder

For a number of years we have used an adaptation of Komnick's KSb(OH)6-OsO4 fixation method for the localization of sodium in tissues in order to study transporting epithelia under a number of different conditions. We have shown that in actively transporting rabbit gallbladder epithelium, large quantities of NaSb(OH)6 precipitate are found in the distended intercellular compartment, while localization of precipitate is confined to the inner side of the lateral plasma membrane in inactive gallbladder epithelium. A similar pattern of distribution of precipitate has been demonstrated in human and rabbit colon in active and inactive states and in the inactive colonic epithelium of hibernating frogs.

Transcellular sodium transport and intracellular sodium activities in rabbit gallbladder

1986 ◽  
Vol 251 (1) ◽  
pp. G155-G159
Author(s):  
W. M. Moran ◽  
R. L. Hudson ◽  
S. G. Schultz
Keyword(s):  
Small Intestine ◽  
Apical Membrane ◽  
Basolateral Membrane ◽  
Gallbladder Epithelium ◽  
Na Transport ◽  
Twofold Increase ◽  
Average Value ◽  
Intracellular Na Activity ◽  
Rabbit Gallbladder ◽  
Sustained Increase

This study was designed to explore the relation between the rate of transcellular active Na+ transport by rabbit gallbladder epithelium, JNa, and the intracellular Na+ activity, (Na)c; the latter was determined by use of highly selective Na+ microelectrodes. The underlying strategy was based on the well-established observation that JNa is stimulated by the presence of bicarbonate in the bathing solutions. Our results confirm previous observations that the addition of bicarbonate to the bathing solutions results in a twofold increase in JNa. In the absence of bicarbonate, (Na)c averaged 16 mM. Within 2–4 min after the addition of bicarbonate to both bathing solutions, (Na)c increased to an average value of 22 mM and then gradually declined and by 15 min did not differ significantly from the value observed in the absence of bicarbonate. Thus, a twofold increase in JNa is not associated with an increase in (Na)c. These results are in accord with earlier observations on Necturus urinary bladder and small intestine and contradict the notion that an increase in the rate of active Na+ extrusion from the cell across the basolateral membrane in response to an increase in the rate of Na+ entry across the apical membrane is necessarily the result of a sustained increase in (Na)c.

Cellular ion route in rabbit gallbladder epithelium

1976 ◽  
Vol 3 (1) ◽  
pp. 92-98 ◽  
Author(s):  
S. Hénin ◽  
D. Cremaschi ◽  
G. Meyer ◽  
G. Brivio
Keyword(s):  
Gallbladder Epithelium ◽  
Rabbit Gallbladder

Apical Na+-Cl− Symport in Rabbit Gallbladder Epithelium: A Thiazide-Sensitive Cotransporter (TSC)

2000 ◽  
Vol 176 (1) ◽  
pp. 53-65
Author(s):  
D. Cremaschi ◽  
C. Porta ◽  
G. Bottà ◽  
C. Bazzini ◽  
M.D. Baroni ◽  
...  
Keyword(s):  
Gallbladder Epithelium ◽  
Rabbit Gallbladder
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