Large cation-selective pores from rat liver peroxisomal membranes incorporated to planar lipid bilayers

1986 ◽  
Vol 94 (3) ◽  
pp. 285-291 ◽  
Author(s):  
Pedro Labarca ◽  
Daniel Wolff ◽  
Ubaldo Soto ◽  
Cecilia Necochea ◽  
Federico Leighton
Keyword(s):  
Lipid Bilayers ◽  
Rat Liver ◽  
Planar Lipid Bilayers ◽  
Large Cation

Anion channels in rat liver canalicular plasma membranes reconstituted into planar lipid bilayers

1992 ◽  
Vol 262 (6) ◽  
pp. G1027-G1032 ◽  
Author(s):  
M. Sellinger ◽  
S. A. Weinman ◽  
R. M. Henderson ◽  
A. Zweifach ◽  
J. L. Boyer ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Lipid Bilayers ◽  
Rat Liver ◽  
Membrane Vesicles ◽  
Planar Lipid Bilayers ◽  
Anion Channels ◽  
Plasma Membrane Vesicles ◽  
Patch Clamp Technique ◽  
Bile Formation ◽  
Cl Conductance

Previous studies from this laboratory have demonstrated a Cl(-)-HCO3- exchanger and have provided evidence for a Cl- conductance in rat liver canalicular plasma membrane vesicles. To further investigate the apical Cl- conductance, we performed single-channel analysis after incorporation of canalicular liver plasma membrane vesicles into planar lipid bilayers. This was necessary, because the canalicular membrane is not accessible for the patch-clamp technique. Two types of anion channels could be identified (30- and 90-pS conductance) corresponding to the class of small and intermediate channels, respectively. The kinetics of the small channel were found to be voltage dependent with a maximum for the open probability at -20 mV. In contrast, intermediate channel kinetics were voltage independent. The anion channels described above could allow electrogenic Cl- efflux, to compensate Cl- influx via the electroneutral Cl(-)-HCO3- exchanger. Further studies will be required to prove their functional importance in bile formation.

scholarly journals Saturation behavior of single, amiloride-sensitive Na+ channels in planar lipid bilayers

1984 ◽  
Vol 46 (6) ◽  
pp. 831-835 ◽  
Author(s):  
L. Olans ◽  
S. Sariban-Sohraby ◽  
D.J. Benos
Keyword(s):  
Lipid Bilayers ◽  
Planar Lipid Bilayers ◽  
Na Channels

scholarly journals Stimulation‐dependent gating of TRPM3 channel in planar lipid bilayers

2015 ◽  
Vol 30 (3) ◽  
pp. 1306-1316 ◽  
Author(s):  
Kunitoshi Uchida ◽  
Lusine Demirkhanyan ◽  
Swapna Asuthkar ◽  
Alejandro Cohen ◽  
Makoto Tominaga ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Planar Lipid Bilayers

Ryanodine receptor dysfunction in porcine stunned myocardium

1997 ◽  
Vol 273 (2) ◽  
pp. H796-H804 ◽  
Author(s):  
C. Valdivia ◽  
J. O. Hegge ◽  
R. D. Lasley ◽  
H. H. Valdivia ◽  
R. Mentzer
Keyword(s):  
Coronary Artery ◽  
Lipid Bilayers ◽  
Ryanodine Receptor ◽  
Myocardial Stunning ◽  
Single Channel ◽  
Stunned Myocardium ◽  
Wall Thickening ◽  
Planar Lipid Bilayers ◽  
Open Probability ◽  
Single Channel Recordings

We investigated the effects of myocardial stunning on the function of the two main Ca2+ transport proteins of the sarcoplasmic reticulum (SR), the Ca(2+)-adenosinetriphosphatase and the Ca(2+)-release channel or ryanodine receptor. Regional myocardial stunning was induced in open-chest pigs (n = 6) by a 10-min occlusion of the left anterior descending coronary artery (LAD) and 2 h reperfusion. SR vesicles isolated from the LAD-perfused region (stunned) and the normal left circumflex coronary artery (LC)-perfused region were used to assess the oxalate-supported 45Ca2+ uptake, [3H]ryanodine binding, and single-channel recordings of ryanodine-sensitive Ca(2+)-release channels in planar lipid bilayers. Myocardial stunning decreased LAD systolic wall thickening to 20% of preischemic values. The rate of SR 45Ca2+ uptake in the stunned LAD bed was reduced by 37% compared with that of the normal LC bed (P < 0.05). Stunning was also associated with a 38% reduction in the maximal density of high-affinity [3H]ryanodine binding sites (P < 0.05 vs. normal LC) but had no effect on the dissociation constant. The open probability of ryanodine-sensitive Ca(2+)-release channels determined by single channel recordings in planar lipid bilayers was 26 +/- 2% for control SR (n = 33 channels from 3 animals) and 14 +/- 2% for stunned SR (n = 21 channels; P < 0.05). This depressed activity of SR function observed in postischemic myocardium could be one of the mechanisms underlying myocardial stunning.

Properties of Chicken Lens MIP Channels Reconstituted into Planar Lipid Bilayers

1996 ◽  
Vol 154 (3) ◽  
pp. 239-249 ◽  
Author(s):  
E. Modesto ◽  
P. D. Lampe ◽  
M. C. Ribeiro ◽  
D. C. Spray , A. C. Campos de Carvalho
Keyword(s):  
Lipid Bilayers ◽  
Planar Lipid Bilayers
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