Sulfhydryl-reactive heavy metals increase cell membrane K+ and Ca2+ transport in renal proximal tubule

1990 ◽  
Vol 113 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Bruce C. Kone ◽  
Robert M. Brenner ◽  
Steven R. Gullans
Keyword(s):  
Heavy Metals ◽  
Cell Membrane ◽  
Proximal Tubule ◽  
Renal Proximal Tubule

Short- and Long-Term Influences of Heavy Metals on Anionic Drug Efflux from Renal Proximal Tubule

2002 ◽  
Vol 301 (2) ◽  
pp. 578-585 ◽  
Author(s):  
Sylvie A. Terlouw ◽  
Claudia Graeff ◽  
Pascal H. E. Smeets ◽  
Gert Fricker ◽  
Frans G. M. Russel ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Proximal Tubule ◽  
Renal Proximal Tubule ◽  
Drug Efflux ◽  
Short And Long Term

scholarly journals Rheogenic transport in the renal proximal tubule.

1983 ◽  
Vol 82 (6) ◽  
pp. 819-851 ◽  
Author(s):  
H Sackin ◽  
E L Boulpaep
Keyword(s):  
Cell Membrane ◽  
Proximal Tubule ◽  
Basolateral Membrane ◽  
Electrical Potential ◽  
Renal Proximal Tubule ◽  
Coupling Ratio ◽  
Electrical Equivalent Circuit ◽  
Nernst Potential ◽  
Intracellular Na Activity ◽  
Activity Measurements

The electrophysiology of the renal Na-K ATPase was studied in isolated perfused amphibian proximal tubules during alterations in bath (serosal) potassium. Intracellular and extracellular ionic activity measurements permitted continuous evaluation of the Nernst potentials for Na+, K+, and Cl- across the basolateral membrane. The cell membrane and transepithelial potential differences and resistances were also determined. Return of K to the basal (serosal) solution after a 20-min incubation in K-free solution hyperpolarized the basolateral membrane to an electrical potential that was more negative than the Nernst potential for either Na, Cl, or K. This constitutes strong evidence that at least under stimulated conditions the Na-K ATPase located at the basolateral membrane of the renal proximal tubule mediates a rheogenic process which directly transfers net charge across the cell membrane. Interpretation of these data in terms of an electrical equivalent circuit permitted calculation of both the rheogenic current and the Na/K coupling ratio of the basolateral pump. During the period between 1 and 3 min after pump reactivation by return of bath K, the basolateral rheogenic current was directly proportional to the intracellular Na activity, and the pump stoichiometry transiently exceeded the coupling ratio of 3Na to 2K reported in other preparations.

The Fine Structure of Rat Renal Microbodies and Cytoplasmic Bodies Following Perfusion Fixation

1973 ◽  
Vol 31 ◽  
pp. 620-621
Author(s):  
J. M. Barrett ◽  
P. M. Heidger
Keyword(s):  
Fine Structure ◽  
Proximal Tubule ◽  
Rat Kidney ◽  
Renal Proximal Tubule ◽  
Convincing Evidence ◽  
Cytoplasmic Bodies ◽  
Rat Renal Proximal Tubule ◽  
Renal Proximal Tubule Cells ◽  
Perfusion Fixation

Microbodies have received extensive morphological and cytochemical investigation since they were first described by Rhodin in 1954. To our knowledge, however, all investigations of microbodies and cytoplasmic bodies of rat renal proximal tubule cells have employed immersion fixation. Tisher, et al. have shown convincing evidence of fine structural alteration of microbodies in rhesus monkey kidney following immersion fixation; these alterations were not encountered when in vivo intravascular perfusion was employed. In view of these studies, and the fact that techniques for perfusion fixation have been established specifically for the rat kidney by Maunsbach, it seemed desirable to employ perfusion fixation to study the fine structure and distribution of microbodies and cytoplasmic bodies within the rat renal proximal tubule.

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