Membrane potential and surface potential in mitochondria: Uptake and binding of lipophilic cations

1984 ◽  
Vol 81 (2) ◽  
pp. 127-138 ◽  
Author(s):  
Hagai Rottenberg
Keyword(s):  
Membrane Potential ◽  
Surface Potential ◽  
Lipophilic Cations

scholarly journals Impact of Ciprofloxacin and Chloramphenicol on the Lipid Bilayer ofStaphylococcus aureus: Changes in Membrane Potential

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Paulina L. Páez ◽  
María C. Becerra ◽  
Inés Albesa
Keyword(s):  
Staphylococcus Aureus ◽  
Membrane Potential ◽  
Cell Membrane ◽  
Surface Potential ◽  
Binding Sites ◽  
Ofstaphylococcus Aureus ◽  
Bacterial Membranes ◽  
Electrostatic Surface Potential ◽  
Number Of Binding Sites ◽  
Resistant Strains

The present study was undertaken to explore the interaction of ciprofloxacin and chloramphenicol with bacterial membranes in a sensitive and in a resistant strains ofStaphylococcus aureusby using 1-anilino-8-naphthalene sulfonate (ANS). The binding of this probe to the cell membrane depends on the surface potential, which modulates the binding constant to the membrane. We observed that these antibiotics interacted with the bilayer, thus affecting the electrostatic surface potential. Alterations caused by antibiotics on the surface of the bacteria were accompanied by a reduction in the number of binding sites and an increase in the ANS dissociation constant in the sensitive strain, whereas in the ciprofloxacin-resistant strain no significant changes were detected. The changes seen in the electrostatic surface potential generated in the membrane ofS. aureusby the antibiotics provide new aspects concerning their action on the bacterial cell.

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