Effect of phospholipid surface charge on the conductance and gating of a Ca2+-activated K+ channel in planar lipid bilayers

1985 ◽  
Vol 83 (3) ◽  
pp. 273-282 ◽  
Author(s):  
Edward Moczydlowski ◽  
Osvaldo Alvarez ◽  
Cecilia Vergara ◽  
Ramon Latorre
Keyword(s):  
Surface Charge ◽  
Lipid Bilayers ◽  
K Channel ◽  
Planar Lipid Bilayers

Single K+-channel current measurements from brain synaptosomes in lipid bilayers

1983 ◽  
Vol 245 (1) ◽  
pp. C151-C156 ◽  
Author(s):  
M. T. Nelson ◽  
M. Roudna ◽  
E. Bamberg
Keyword(s):  
Ion Channels ◽  
Lipid Bilayers ◽  
Single Channel ◽  
Mammalian Brain ◽  
K Channel ◽  
Kinetic Properties ◽  
Nerve Terminals ◽  
Planar Lipid Bilayers ◽  
Presynaptic Nerve Terminals ◽  
Single Channel Currents

Ion channels from a rat brain preparation enriched in presynaptic nerve terminals (synaptosomes) were incorporated into planar lipid bilayers. Experiments examined macroscopic (channel-ensemble) currents as well as single-channel currents. Four single-channel conductances (ranging from 10 to 40 pS) were usually observed, each with distinct kinetic properties. All the observed channels selected for K+ over Cl-. These K+ channels may contribute to the resting K+ conductance of brain nerve terminals. Furthermore, this report demonstrates that the properties of ion channels from mammalian brain can be studied in planar lipid bilayers and suggests that this system can be readily extended to many additional investigations on the electrical properties of brain membranes.

Reconstitution of the voltage-gated K+ channel KAT1 in planar lipid bilayers

2008 ◽  
Vol 10 (10) ◽  
pp. 1509-1512 ◽  
Author(s):  
Shunsuke Ozaki ◽  
Shiho Aoki ◽  
Takao Hibi ◽  
Kenji Kano ◽  
Osamu Shirai
Keyword(s):  
Lipid Bilayers ◽  
K Channel ◽  
Planar Lipid Bilayers ◽  
Voltage Gated

scholarly journals Conduction, Blockade and Gating in a Ca2+ -activated K+ Channel Incorporated into Planar Lipid Bilayers

1984 ◽  
Vol 45 (1) ◽  
pp. 73-76 ◽  
Author(s):  
Cecilia Vergara ◽  
Edward Moczydlowski ◽  
Ramon Latorre
Keyword(s):  
Lipid Bilayers ◽  
K Channel ◽  
Planar Lipid Bilayers

scholarly journals Saturation behavior of single, amiloride-sensitive Na+ channels in planar lipid bilayers

1984 ◽  
Vol 46 (6) ◽  
pp. 831-835 ◽  
Author(s):  
L. Olans ◽  
S. Sariban-Sohraby ◽  
D.J. Benos
Keyword(s):  
Lipid Bilayers ◽  
Planar Lipid Bilayers ◽  
Na Channels

scholarly journals Stimulation‐dependent gating of TRPM3 channel in planar lipid bilayers

2015 ◽  
Vol 30 (3) ◽  
pp. 1306-1316 ◽  
Author(s):  
Kunitoshi Uchida ◽  
Lusine Demirkhanyan ◽  
Swapna Asuthkar ◽  
Alejandro Cohen ◽  
Makoto Tominaga ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Planar Lipid Bilayers
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