Transmembrane electrophoresis of 8-anilino-1-naphthalenesulfonate through egg lecithin liposome membranes

1975 ◽  
Vol 24 (1) ◽  
pp. 237-248 ◽  
Author(s):  
Nigel Gains ◽  
Alan P. Dawson
Keyword(s):  
Egg Lecithin ◽  
Lecithin Liposome

Calcium Fortification of Soy Milk with Calcium-Lecithin Liposome System

1984 ◽  
Vol 49 (4) ◽  
pp. 1111-1112 ◽  
Author(s):  
MOTOHIKO HIROTSUKA ◽  
HITOSHI TANIGUCHI ◽  
HIROSHI NARITA ◽  
MAKOTO KITO
Keyword(s):  
Soy Milk ◽  
Liposome System ◽  
Lecithin Liposome

HYDROLYSIS OF LECITHIN BY PLANT PLASTID ENZYMES

1955 ◽  
Vol 33 (1) ◽  
pp. 575-589 ◽  
Author(s):  
Morris Kates
Keyword(s):  
Phosphatidic Acid ◽  
Inorganic Phosphate ◽  
Organic Phosphate ◽  
Water Soluble ◽  
Lag Phase ◽  
Carrot Root ◽  
First Order ◽  
Spinach Chloroplasts ◽  
Egg Lecithin ◽  
Hydrolysis Of

Enzymatic liberation of choline from egg lecithin by plastid fractions from sugar beet, spinach, and cabbage leaves and from carrot root was a rapid, first order reaction (up to 70% hydrolysis), and was not preceded by a lag phase. None of the choline-containing products of lecithin degradation (lysolecithin, glycerylphosphorylcholine, or phosphorylcholine) lost choline on incubation with spinach chloroplasts. Inorganic phosphate liberation from lecithin by the plastids was preceded by a lag phase and was much slower than choline liberation. Spinach chloroplasts catalyzed the liberation of inorganic phosphate from L-α-phosphatidic acid and from L-α-glycerophosphate. The water-soluble organic phosphate liberated from lecithin by spinach chloroplasts was identified chromatographically as phosphorylcholine. The ether-soluble organic phosphate produced during the hydrolysis of egg lecithin by carrot plastids was isolated and identified as L-α-phosphatidic acid. These observations suggest that the enzymatic hydrolysis of lecithin by plant plastids involves the following reactions: (1) lecithin → L-α-phosphatidic acid + choline; (2) L-α-phosphatidic acid → inorganic phosphate + diglyceride and/or (3) L-α-phosphatidic acid → glycerophosphate + fatty acids and (4) glycerophosphate → inorganic phosphate + glycerol; and (5) lecithin → phosphorylcholine + diglyceride. The L-α-structure for egg lecithin was confirmed.

scholarly journals OXIDATIVE STRESS MARKERS IN THE LUNGS UNDER EXPERIMENTAL HYPEROXIA WITH INHALED LIPOSOMES BASED ON EGG LECITHIN

Biomeditsina ◽  
2019 ◽  
pp. 49-58
Author(s):  
I. L. Kotovich ◽  
Zh. A. Rutkovskaya ◽  
A. D. Taganovich
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Alpha Tocopherol ◽  
Oxygen Species ◽  
Glutathione Peroxidase Activity ◽  
Diene Conjugates ◽  
Hyperoxia Exposure ◽  
Egg Lecithin

Oxidative stress is considered to be a factor leading to lung damage in premature infants. The aim of this study was to investigate the effect of inhaled antioxidants incorporated into egg lecithin liposomes on the indicators of oxidative stress in the lungs of newborn guinea pigs under experimental hyperoxia (3 days). Bronchoalveolar lavage fl uid (BALF) was used as a material for the study. Under hyperoxia exposure, inhalation of liposomes containing N-acetylcysteine and alpha-tocopherol contributed to the suppression of the reactive oxygen species production by cells, normalization of glutathione peroxidase activity and carbonyls content, while not affecting the level of diene conjugates in BALF. The introduction of retinoid-containing liposomes (retinol and retinoic acid) under hyperoxia was accompanied by normalization of glutathione peroxidase activity as well as the content of protein oxidation products in BALF, while the generation of reactive oxygen species remained enhanced, and the diene conjugates and thiobarbituric acid reactive products exceeded the levels in animals exposed to hyperoxia alone. Thus, the inhaled liposomes containing retinoids and egg lecithin exhibit not only anti-, but also a prooxidant effect in the lungs under hyperoxia exposure, unlike the liposomal forms of N-acetylcysteine and alpha-tocopherol.

scholarly journals PREPARATION, PHYSICAL CHARACTERIZATION, AND PHARMACOKINETIC STUDY OF DOCETAXEL NANOCRYSTALS

2019 ◽  
pp. 238-244
Author(s):  
ARVIND GANNIMITTA ◽  
PRATHIMA SRINIVAS ◽  
VENKATESHWAR REDDY A ◽  
PEDIREDDI SOBHITA RANI
Keyword(s):  
Particle Size ◽  
Drug Release ◽  
Zeta Potential ◽  
Water Solubility ◽  
Release Kinetics ◽  
Tween 80 ◽  
Fickian Diffusion ◽  
Sustained Drug Release ◽  
Egg Lecithin

Objective: The main objective of this study was to prepare and evaluate the nanocrystal formulation of docetaxel. Methods: Docetaxel nanocrystals were formulated to improve the water solubility. Docetaxel nanocrystals were prepared by nanoprecipitation method using Tween 80, egg lecithin, and povidone C-12 as stabilizers and poly(lactic-co-glycolic acid) (PLGA) as polymer in acceptable limits. A total of 16 formulations were prepared by changing stabilizer and polymer ratios. The prepared nanocrystals were characterized by particle size, zeta potential, crystalline structure, surface morphology, assay, saturation solubility, and in vitro drug release. Results: Based on particle size, polydispersity index, and zeta potential data, four formulations were optimized. The formulation containing Tween 80 as stabilizer has shown lowest particle size and better drug release than the formulations containing egg lecithin and povidone C-12 as stabilizers. The formulation containing Tween 80 and PLGA has shown still lower sized particles than the Tween 80 alone and exhibited prolonged sustained drug release. The release kinetics of formulations containing Tween 80 and PLGA followed zero-order release kinetics and formulations containing egg lecithin and povidone C-12 followed Higuchi diffusion (non-Fickian). Conclusion: From the study, we concluded that as the type and concentration of stabilizer changed the size and shape of the crystals were also changed and the formulations showed sustained drug release with non-Fickian diffusion.

Protective Effect of Amphiphilic Ammonium Salts on the Oxidation of Lecithin Liposomes

1995 ◽  
Vol 50 (11-12) ◽  
pp. 840-844
Author(s):  
J Gabrielska ◽  
H Kleszczyriska ◽  
S Przestalski
Keyword(s):  
Chain Length ◽  
Alkyl Chain ◽  
Uv Light ◽  
Alkyl Chain Length ◽  
Ammonium Salts ◽  
Ferrous Ions ◽  
Liposome Membrane ◽  
Similar Dependence ◽  
Substituted Ammonium Salts ◽  
Lecithin Liposome

Abstract The inhibition effect of selected amphiphilic quaternary alkyl-substituted ammonium salts on oxidation of the lecithin liposome membrane was studied, induced by ultraviolet light and Fe2+ ions with ascorbic acid added. The salts differed in alkyl chain length Ri having the following alkyl substitutents: R1 = C9H19; R2 = C10H21; R3 = C12H25; R4 = CH2OC14H29; R5 = CH2OC16H33. It was found that all the salts used induced inhibition in oxidation of the liposome membrane, both that induced by UV light and ferrous ions. The antioxidant activity of the salts studied, dependent on the chain length and concentration, can be represented by the relation R1 < R2< R3 < R4 < R5. A similar dependence was obtained when the hemolytic activity of compounds was studied. Activity of salts increase with alkyl chain length.

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