Structure and permeability of goblet cell tight junctions in rat small intestine

1982 ◽  
Vol 66 (1) ◽  
pp. 145-157 ◽  
Author(s):  
James L. Madara ◽  
Jerry S. Trier
Keyword(s):  
Small Intestine ◽  
Tight Junctions ◽  
Goblet Cell ◽  
Rat Small Intestine

Goblet Cell Mucin of Rat Small Intestine. Chemical and Physical Characterization

1973 ◽  
Vol 51 (8) ◽  
pp. 1154-1166 ◽  
Author(s):  
Janet F. Forstner ◽  
Inderjit Jabbal ◽  
Gordon G. Forstner
Keyword(s):  
Molecular Weight ◽  
Small Intestine ◽  
Goblet Cell ◽  
Disulfide Bonds ◽  
Axial Ratio ◽  
Dry Weight ◽  
Subunit Structure ◽  
Rat Small Intestine ◽  
Minor Components ◽  
Sulfur Containing

Goblet cell mucin (GCM) of rat small intestine has been isolated previously, and its location established by immunofluorescence techniques. In the present study, GCM was characterized more fully by chemical and physical methods. It was found to be a flexible negatively charged macromolecule with a molecular weight of about 2.0 × 106, an intrinsic viscosity of 15.3 dl/g, and an axial ratio of about 225:1. Its composition was protein 12%, total hexose 23%, hexosamine 22.4%, sialic acid 10%, fucose 6.6%, and sulfate < 1% of the dry weight. It contained approximately 34 disulfide bonds per molecule. Like most mucin glycoproteins it was rich in serine, threonine, and proline (45.5 mol %) and poor in hydrophobic and sulfur containing amino acids. One major and two minor components were identified by acrylamide disc gel electrophoresis and analytic ultracentrifugation. The components appeared to represent different molecular weight species of GCM. No evidence of subunit structure could be obtained using a variety of techniques, including the disruption of ionic, hydrophobic, and disulfide bonds by detergents, denaturants, or reducing agents.

scholarly journals Localization of the Retinol-dependent Fucose-Glycopeptide in the Goblet Cell of the Rat Small Intestine

1971 ◽  
Vol 246 (18) ◽  
pp. 5762-5765
Author(s):  
Luigi De Luca ◽  
Marlis Schumacher ◽  
David P. Nelson
Keyword(s):  
Small Intestine ◽  
Goblet Cell ◽  
Rat Small Intestine

In situ demonstration of migration of dendritic cells released from rat small intestine to mesenteric lymph nodes

2001 ◽  
Vol 120 (5) ◽  
pp. A183-A183
Author(s):  
H KOBAYASHI ◽  
H NAGATA ◽  
S MIURA ◽  
T AZUMA ◽  
H SUZUKI ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Small Intestine ◽  
Lymph Nodes ◽  
Rat Small Intestine ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph

Anti-inflammatory effect of STW 5, STW 6 and isolated extracts on the rat small intestine

2008 ◽  
Vol 29 (S 1) ◽  
Author(s):  
K Nieber ◽  
S Michael ◽  
K Grötzinger ◽  
JW Rauwald ◽  
O Kelber
Keyword(s):  
Small Intestine ◽  
Rat Small Intestine ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Oral vanadate reduces Na(+)-dependent glucose transport in rat small intestine

Diabetes ◽  
1993 ◽  
Vol 42 (8) ◽  
pp. 1126-1132 ◽  
Author(s):  
K. L. Madsen ◽  
V. M. Porter ◽  
R. N. Fedorak
Keyword(s):  
Small Intestine ◽  
Glucose Transport ◽  
Rat Small Intestine
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