The role of the lateral intercellular spaces and solute polarization effects in the passive flow of water across the rabbit gallbladder

1972 ◽  
Vol 7 (1) ◽  
pp. 198-219 ◽  
Author(s):  
Ernest M. Wright ◽  
Anthony P. Smulders ◽  
John D. Tormey
Keyword(s):  
Intercellular Spaces ◽  
Polarization Effects ◽  
Passive Flow ◽  
Rabbit Gallbladder ◽  
Lateral Intercellular Spaces ◽  
Solute Polarization

Role of tight-junctional pathways in bile salt-induced increases in colonic permeability

1983 ◽  
Vol 245 (6) ◽  
pp. G816-G823 ◽  
Author(s):  
R. W. Freel ◽  
M. Hatch ◽  
D. L. Earnest ◽  
A. M. Goldner
Keyword(s):  
Bile Salt ◽  
Ussing Chamber ◽  
Intercellular Spaces ◽  
Low Concentrations ◽  
Lateral Intercellular Spaces ◽  
In Series ◽  
Junctional Complexes ◽  
Dose Dependent ◽  
Tracer Experiments

The effects of a dihydroxy bile salt, taurochenodeoxycholate (TCDC), on the permeability and conductance of isolated, short-circuited segments of the rabbit descending colon were examined using conventional Ussing chamber techniques. Increasing concentrations of TCDC (1℃4 mM) produced dose-dependent increases in sodium backflux (JNas leads to m) and tissue conductance (Gt) when applied to either the mucosal or serosal salines. However, mucosal addition was twice as potent in increasing JNas leads to m and Gt at 4 mM. Tracer experiments indicated that the transepithelial serosal-to-mucosal fluxes of sodium and mannitol are via an aqueous, unrestricted, free-solution pathway, while albumin movements are restricted through this pathway both in the absence and presence of mucosal TCDC. The changes in JNas leads to m, JMans leads to m, and Gt caused by 4 mM mucosal TCDC were largely reversed by rinsing the mucosal chamber with fresh buffer. It was also observed that osmotically induced volume flows in the serosal-to-mucosal direction could offset or reverse the changes in Gt produced by 2 mM mucosal TCDC, suggesting that the enhanced conductance pathway is in series with the lateral intercellular spaces. Taken together, these results suggest that low concentrations of TCDC alter the integrity of tight-junctional complexes between the epithelial cells of the rabbit colon.

scholarly journals Pseudo-streaming potentials in Necturus gallbladder epithelium. II. The mechanism is a junctional diffusion potential.

1992 ◽  
Vol 99 (3) ◽  
pp. 317-338 ◽  
Author(s):  
L Reuss ◽  
B Simon ◽  
C U Cotton
Keyword(s):  
Time Course ◽  
Bathing Solution ◽  
Intercellular Spaces ◽  
Similar Time ◽  
Streaming Potentials ◽  
Osmotic Water ◽  
Lateral Intercellular Spaces ◽  
Transepithelial Voltage ◽  
Time Courses ◽  
Good Agreement

The mechanisms of apparent streaming potentials elicited across Necturus gallbladder epithelium by addition or removal of sucrose from the apical bathing solution were studied by assessing the time courses of: (a) the change in transepithelial voltage (Vms). (b) the change in osmolality at the cell surface (estimated with a tetrabutylammonium [TBA+]-selective microelectrode, using TBA+ as a tracer for sucrose), and (c) the change in cell impermeant solute concentration ([TMA+]i, measured with an intracellular double-barrel TMA(+)-selective microelectrode after loading the cells with TMA+ by transient permeabilization with nystatin). For both sucrose addition and removal, the time courses of Vms were the same as the time courses of the voltage signals produced by [TMA+]i, while the time courses of the voltage signals produced by [TBA+]o were much faster. These results suggest that the apparent streaming potentials are caused by changes of [NaCl] in the lateral intercellular spaces, whose time course reflects the changes in cell water volume (and osmolality) elicited by the alterations in apical solution osmolality. Changes in cell osmolality are slow relative to those of the apical solution osmolality, whereas lateral space osmolality follows cell osmolality rapidly, due to the large surface area of lateral membranes and the small volume of the spaces. Analysis of a simple mathematical model of the epithelium yields an apical membrane Lp in good agreement with previous measurements and suggests that elevations of the apical solution osmolality elicit rapid reductions in junctional ionic selectivity, also in good agreement with experimental determinations. Elevations in apical solution [NaCl] cause biphasic transepithelial voltage changes: a rapid negative Vms change of similar time course to that of a Na+/TBA+ bi-ionic potential and a slow positive Vms change of similar time course to that of the sucrose-induced apparent streaming potential. We conclude that the Vms changes elicited by addition of impermeant solute to the apical bathing solution are pseudo-streaming potentials, i.e., junctional diffusion potentials caused by salt concentration changes in the lateral intercellular spaces secondary to osmotic water flow from the cells to the apical bathing solution and from the lateral intercellular spaces to the cells. Our results do not support the notion of junctional solute-solvent coupling during transepithelial osmotic water flow.

scholarly journals Studies on the Electrical Potential Profile across Rabbit Ileum

1971 ◽  
Vol 57 (6) ◽  
pp. 639-663 ◽  
Author(s):  
Richard C. Rose ◽  
Stanley G. Schultz
Keyword(s):  
Amino Acids ◽  
Brush Border ◽  
Electrical Potential ◽  
Metabolic Inhibitors ◽  
Electrical Potential Difference ◽  
Intercellular Spaces ◽  
Cell Interior ◽  
Rabbit Ileum ◽  
Lateral Intercellular Spaces ◽  
Rapid Replacement

When isolated strips of mucosal rabbit ileum are bathed by physiological electrolyte solution the electrical potential difference (PD) across the brush border (ψmc) averages 36 mv, cell interior negative. Rapid replacement of Na in the mucosal solution with less permeant cations, Tris or choline, results in an immediate hyperpolarization of ψmc. Conversely, replacement of choline in the mucosal solution with Na results in an abrupt depolarization of ψmc. These findings indicate that Na contributes to the conductance across the brush border. The presence of actively transported sugars or amino acids in the mucosal solution brings about a marked depolarization of ψmc and a smaller increase in the transmural PD (Δψms). It appears that the Na influx that is coupled to the influxes of amino acids and sugars is electrogenic and responsible for the depolarization of ψmc. Under control conditions Δψms can be attributed to the depolarization of ψmc together with the presence of a low resistance transepithelial shunt, possibly the lateral intercellular spaces. However, quantitatively similar effects of amino acids on ψmc are also seen in tissues poisoned with metabolic inhibitors or ouabain. Under these conditions Δψmc is much smaller than under control conditions. Thus, the depolarization of ψmc might not account for the entire Δψms, observed in nonpoisoned tissue. An additional electromotive force which is directly coupled to metabolic processes might contribute to the normal Δψms.

Cryo-scanning electron microscopy (CSEM) in the advancement of functional plant biology: energy dispersive X-ray microanalysis (CEDX) applications

2010 ◽  
Vol 37 (11) ◽  
pp. 1011 ◽  
Author(s):  
Margaret E. McCully ◽  
Martin J. Canny ◽  
Cheng X. Huang ◽  
Celia Miller ◽  
Frank Brink
Keyword(s):  
Single Cells ◽  
Energy Dispersive ◽  
Intercellular Spaces ◽  
Root Pressure ◽  
Solute Content ◽  
X Ray ◽  
Plant Parts ◽  
Specific Analysis ◽  
Vesicular Arbuscular

The capacity to make measurements of elemental concentrations at the level of single cells by energy dispersive X-ray microanalysis of cryo-fixed, inherently-hydrated plant parts (CEDX) is changing or extending our understanding of many plant functions. We include in this review a wide-ranging catalogue of studies that have used CEDX which provides access to the literature on elements measured, plants and tissues studied, techniques used, level of quantitation and the significant findings. These findings include new perspectives on the following areas: salt tolerance; xylem maturation and solute content, root pressure and embolism refilling; the contents of intercellular spaces; sequestration of toxic elements; biomineralisation with silicon; movement of tracer homologues of native cations; indirect localisation of molecules with a distinctive element component; transfer of nutrients from vesicular-arbuscular (VA) mycorrhizas; the role of mucilages in protection and in generating mechanical force. In an Appendix we discuss the procedures involved in CEDX: cryo-fixation, specimen planing, etching, elemental quantitation and mapping. Limitations on sample numbers, elements measurable, spatial resolution, sensitivity and threshold concentrations quantifiable are outlined. A brief discussion of the potential of emerging technologies for cell-specific analysis of cryo-fixed, hydrated specimens is included. In the Accessory Publication we list our standard protocol for CEDX.

THEORETICAL ASPECTS OF THE POLARIZABILITY OF THE NUCLEON

1993 ◽  
Vol 08 (30) ◽  
pp. 5267-5303 ◽  
Author(s):  
A.I. L’VOV
Keyword(s):  
Perturbation Theory ◽  
Chiral Perturbation Theory ◽  
Gauge Invariance ◽  
Degrees Of Freedom ◽  
Dominant Role ◽  
Energy Shift ◽  
Chiral Perturbation ◽  
Polarization Effects ◽  
Consistent Treatment

Two aspects of the nucleon electromagnetic polarizabilities, αN and βN, are discussed. The first one is the interrelation between static and Compton polarizabilities. Relativistically consistent treatment of the polarization effects based on effective Lagrangians results in the conclusion that the Compton polarizabilities, rather than the static ones, drive the energy shift of the nucleon in external fields. The cluster substructure of the static polarizabilities is shown to be crucial for consistent calculations of the polarizabilities. The second aspect concerns the dominant role of pion degrees of freedom in αN as seen through dispersion relations, chiral perturbation theory, and constituent models. The reported calculations of αN within soliton models are shown to be inconsistent with gauge invariance. The difficulties in explaining the observed large diamagnetic component of βN are emphasized.

Sign in / Sign up

Export Citation Format

Share Document