Phagocytic defects

Paul G. Quie; Elaine L. Mills; Linda C. McPhail; Richard B. Johnston

doi:10.1007/bf01857310

Cellular and Plasma-Associated Phagocytic Defects Amongst Iranian Blood Donors

Vox Sanguinis ◽

10.1159/000467569 ◽

1978 ◽

Vol 34 (2) ◽

pp. 92-96

Author(s):

A. Ghavami Nejad ◽

C. Harbour ◽

F. Ala ◽

Nooshin Foroozanjar

Keyword(s):

Blood Donors ◽

Phagocytic Defects

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Cellular and Plasma-Associated Phagocytic Defects Amongst Iranian Blood Donors

Vox Sanguinis ◽

10.1111/j.1423-0410.1978.tb03729.x ◽

1978 ◽

Vol 34 (2) ◽

pp. 92-96

Author(s):

Nooshin Foroozanfar ◽

A. Ghavami Nejad ◽

C. Harbour ◽

F. Ala

Keyword(s):

Blood Donors ◽

Phagocytic Defects

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Clinical and Laboratory Characteristics of Primary Immunodeficiency Patients from a Tertiary Care Center in Pakistan

Journal of the Pakistan Medical Association ◽

10.47391/jpma.512 ◽

2020 ◽

pp. 1-15

Author(s):

Hamid Nawaz Tipu ◽

Dawood Ahmed

Keyword(s):

Primary Immunodeficiency ◽

Clinical Presentation ◽

Care Center ◽

Tertiary Care ◽

Severe Combined Immunodeficiency ◽

Laboratory Data ◽

Genetic Diagnosis ◽

Warning Signs ◽

Combined Immunodeficiency ◽

Phagocytic Defects

Abstract Objective: The aim of this study was to describe and identify clinical presentation of primary immunodeficiency disorders (PIDs). Characteristic quantitative and qualitative immunological abnormalities have been described which help in establishing a definitive PID diagnosis. Methods: Cross sectional study in Immunology department, Armed Forces Institute of Pathology, Rawalpindi, Pakistan, from Jan 2016 to Dec 2018. Sixty patients of different PIDs including humoral defects, combined immunodeficiency, phagocytic defects and other miscellaneous disorders, were diagnosed over a period of 3 years in our institute. Their clinical presentation and laboratory data are presented in this study. Results: In 3 years, 40 (66%) males and 20 (33%) females were diagnosed, with 13 (21.6%) patients of humoral deficiency, 22 (36.6%) of severe combined immunodeficiency, 18 (30%) of phagocytic defects and 7 (11.6%) of other miscellaneous disorders. Maximum patients belonged to Punjab province, i.e., 23 (38.3%). Their mean age for initiation of symptoms was 7 + 12.6 months, while diagnosis was made at mean age of 26 + 39.28 months, in all groups combined. Respiratory infections were commonest presentation, in 46 (76.6%) patients. Also 46 (76.6%) patients had consanguineous parents. Presence of family history of PID in 27 (45%) patients is not associated with an earlier diagnosis (p 0.955). Each group of patients carried characteristic laboratory findings. Conclusion: PIDs should be suspected in offsprings with warning signs coming from consanguineous parents. There is a need to introduce genetic diagnosis of PIDs in order to timely diagnose less characteristic PID presentations. Continuous...

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Defective monocyte oxidative burst predicts infection in alcoholic hepatitis and is associated with reduced expression of NADPH oxidase

Gut ◽

10.1136/gutjnl-2015-310378 ◽

2016 ◽

Vol 66 (3) ◽

pp. 519-529 ◽

Cited By ~ 23

Author(s):

Nikhil Vergis ◽

Wafa Khamri ◽

Kylie Beale ◽

Fouzia Sadiq ◽

Mina O Aletrari ◽

...

Keyword(s):

Nadph Oxidase ◽

Oxidative Burst ◽

Alcoholic Hepatitis ◽

Negative Regulator ◽

Signalling Pathways ◽

Bacterial Killing ◽

Prednisolone Therapy ◽

Ifn Γ ◽

Phagocytic Defects

ObjectiveIn order to explain the increased susceptibility to serious infection in alcoholic hepatitis, we evaluated monocyte phagocytosis, aberrations of associated signalling pathways and their reversibility, and whether phagocytic defects could predict subsequent infection.DesignMonocytes were identified from blood samples of 42 patients with severe alcoholic hepatitis using monoclonal antibody to CD14. Phagocytosis and monocyte oxidative burst (MOB) were measured ex vivo using flow cytometry, luminometry and bacterial killing assays. Defects were related to the subsequent development of infection. Intracellular signalling pathways were investigated using western blotting and PCR. Interferon-γ (IFN-γ) was evaluated for its therapeutic potential in reversing phagocytic defects. Paired longitudinal samples were used to evaluate the effect of in vivo prednisolone therapy.ResultsMOB, production of superoxide and bacterial killing in response toEscherichia coliwere markedly impaired in patients with alcoholic hepatitis. Pretreatment MOB predicted development of infection within two weeks with sensitivity and specificity that were superior to available clinical markers. Accordingly, defective MOB was associated with death at 28 and 90 days. Expression of the gp91phoxsubunit of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase was reduced in patients with alcoholic hepatitis demonstrating defective MOB. Monocytes were refractory to IFN-γ stimulation and showed high levels of a negative regulator of cytokine signalling, suppressor of cytokine signalling-1. MOB was unaffected by 7 days in vivo prednisolone therapy.ConclusionsMonocyte oxidative burst and bacterial killing is impaired in alcoholic hepatitis while bacterial uptake by phagocytosis is preserved. Defective MOB is associated with reduced expression of NADPH oxidase in these patients and predicts the development of infection and death.

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