Effect of cyclosporine on oxidative phosphorylation and adenylate energy charge of regenerating rat liver

1989 ◽  
Vol 189 (5) ◽  
pp. 313-320 ◽  
Author(s):  
S. Uemoto ◽  
K. Tanaka ◽  
K. Asonuma ◽  
R. Okamura ◽  
Y. Kitakado ◽  
...  
Keyword(s):  
Oxidative Phosphorylation ◽  
Rat Liver ◽  
Energy Charge ◽  
Adenylate Energy Charge ◽  
Regenerating Rat Liver

scholarly journals 407 Regulation of Carbon Flux as a Function of O2 and CO2 Atmospheres in Asparagus Tips

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 514B-514
Author(s):  
S.M. Silva ◽  
R.C. Herner ◽  
R.M. Beaudry
Keyword(s):  
Oxidative Phosphorylation ◽  
Partial Pressure ◽  
Surface Area ◽  
Carbon Metabolism ◽  
Carbon Flux ◽  
Energy Charge ◽  
Low Density Polyethylene ◽  
Low Density ◽  
Adenylate Energy Charge ◽  
Partial Pressures

The purpose of this work was to investigate the influence of O2 and CO2 partial pressures on glycolytic carbon flux, phosphorylated intermediates, phosphate, pyrophosphate, and phosporylated nucleotides in asparagus spears tips stores at 1 °C. The effects of CO2 (0, 5, 10, and 20 kPa) combined with O2 pressures ranging from 0.1 to 16 kPa (1% O2 = 1.013 kPa O2 at 1 atm) were investigated. Spears were enclosed within a low-density polyethylene (LDPE) package (for the 5-, 10-, and 20-kPa CO2 treatments) having a surface area of 462 cm2 and enclosed in 1.95-L glass jars. Low O2 enhanced the interconversion of phosphoenolpyruvate (PEP) to pyruvate (PYR) and F6P to F1,6P2 relative to high O2. When spears tips at 16 kPa O2 were compared to those at harvest, little change occurred in the adenylate or phosphate pools. PPi and ATP contents decreased as the O2 partial pressure declined below 16 kPa O2. In general, as CO2 increased, PPi and ATP decreased, while Pi, ADP, and AMP increased. The adenylate energy charge (AEC) declined with a decline in the O2 partial pressure, declining most rapidly below 2 kPa O2. Low O2 reduced AEC relative to high O2. Increasing CO2 partial pressure reduced AEC, an effect not evident at lower O2. The data suggest low O2 and elevated CO2 impair oxidative phosphorylation and induce nonsustaining carbon metabolism, which may limit asparagus spear survival under O2-deficient conditions.

Effect of 5-nitroindole on adenylate energy charge, oxidative phosphorylation, and lipid peroxidation in rat hepatocytes

1994 ◽  
Vol 48 (7) ◽  
pp. 1483-1492 ◽  
Author(s):  
Marta Dubin ◽  
Patricia H. Carrizo ◽  
Ana M. Biscardi ◽  
Silvia H. Fernandez Villamil ◽  
Andrés O.M. Stoppani
Keyword(s):  
Lipid Peroxidation ◽  
Oxidative Phosphorylation ◽  
Energy Charge ◽  
Rat Hepatocytes ◽  
Adenylate Energy Charge

scholarly journals Relationship Between Protein Synthesis and Secretion in Liver Cells and the State of the Adenine Nucleotide System

1979 ◽  
Vol 32 (3) ◽  
pp. 299 ◽  
Author(s):  
Kaylene Edwards ◽  
Jörg Urban ◽  
Gerhard Schreiber
Keyword(s):  
Protein Synthesis ◽  
Oxidative Phosphorylation ◽  
Protein Secretion ◽  
Liver Cell ◽  
Maximum Rate ◽  
Adenine Nucleotide ◽  
Energy Charge ◽  
Adenylate Energy Charge ◽  
Atp Level ◽  
Atp Pool

Adenine nucleotide levels could be precisely and reproducibly adjusted in liver cell suspensions by partially depleting the ATP pool with D-fructose or glycerol. Thus, it was possible to quantitatively correlate rates of protein synthesis and secretion with intracellular levels of ATP and with derived parameters, such as the adenylate energy charge. Half the maximum rate of incorporation of leucine into protein was observed at an energy charge of 0�80, a ratio of ATP to ADP of 2�6, and an ATP level of 1�05 pmol per g of wet cells. Proteins were secreted with half the maximum rate at an energy charge of 0�85, a ratio of ATP to ADP of 3�1 and an ATP concentration of 1 �1 pmol per gof wet cells. Protein secretion dill not depend on continued synthesis. Inhibitors of oxidative phosphorylation inhibited protein secretion in addition to protein synthesis, in contrast to observations by other authors on liver slices.

Differential Contribution of Oxidative and Glycolytic Energy to Thrombin-Induced Platelet Functions

1979 ◽  
Vol 42 (02) ◽  
pp. 655-665 ◽  
Author(s):  
Bonro Kobayashi ◽  
Yasuko Watanabe ◽  
Naoyuki Takasugi ◽  
Makiko Kurita
Keyword(s):  
Oxidative Phosphorylation ◽  
Blood Platelets ◽  
Energy Charge ◽  
Krebs Cycle ◽  
Energy Condition ◽  
Pulse Labelling ◽  
Adenylate Energy Charge ◽  
Comparable Amount ◽  
Respiratory Inhibitors ◽  
Platelet Aggregates

SummaryWhen washed rabbit-blood platelets were preincubated in an artificial medium in the absence of external substrates, they aggregated in response to a low concentration of thrombin. The aggregation was completely inhibited after the preincubation with respiratory inhibitors. When glucose together with the respiratory inhibitors was added during the incubation, the aggregation was accelerated, whilst it was counteracted when Krebs-cycle substrates were added.ATP was generated actively during the incubation in the absence of external substrates, as well as in the presence of succinate. The ATP-generation was extremely inhibited by oligomycin. When glucose was added during the incubation with the respiratory inhibitor, the comparable amount of ATP with those in the oxidative systems was generated. Metabolic ADP was accumulated in the oxidative systems, particularly in the presence of succinate, in contrast to its low level in the glucose + oligomycin system. The results suggest that the counteraction of the aggregation by the Krebs-cycle substrate is attributed to the low adenylate energy charge. It is suggested that anaerobic glycolysis creates favorable energy condition for aggregation as compared with oxidative phosphorylation, although the washed platelets can be energized to a level above threshold of the aggregation when either one of the two energy generating systems is exerted.After the incubation of the platelets in the presence of thrombin, a higher level of metabolic ATP was observed under glycolytic condition, than under oxidative condition. Pulse-labelling experiments showed that ADP produced during the aggregation was rephosphorylated in a later part of the incubation in the glucose + KCN-fortified system. In the succinate-fortified system, the re-phosphorylation was very slow. The results suggest that oxidative phosphorylation is reduced in platelet aggregates treated with thrombin.

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