Effects of intralipid and hydrocortisone upon human fetal lung cell cultures

1988 ◽  
Vol 188 (6) ◽  
pp. 411-423 ◽  
Author(s):  
H. Kunkelmann ◽  
D. Kleinbauer ◽  
F. Klink ◽  
F. Oberheuser
Keyword(s):  
Cell Cultures ◽  
Fetal Lung ◽  
Human Fetal Lung ◽  
Lung Cell Cultures

Rapid detection of influenza virus infections in human fetal lung diploid cell cultures

1989 ◽  
Vol 18 (3) ◽  
pp. 269-278 ◽  
Author(s):  
P.H. Phipps ◽  
B.G. McCulloch ◽  
H.R. Miller ◽  
E. Rossier
Keyword(s):  
Influenza Virus ◽  
Cell Cultures ◽  
Rapid Detection ◽  
Fetal Lung ◽  
Diploid Cell ◽  
Virus Infections ◽  
Human Fetal Lung

Collagen secretion mediated by NMDR activation in human fetal lung fibroblasts

2010 ◽  
Vol 34 (8) ◽  
pp. S70-S70
Author(s):  
MingJie WANG ◽  
ZiQiang LUO ◽  
Mei LU ◽  
LiHong SHANG ◽  
ShaoJie YUE
Keyword(s):  
Fetal Lung ◽  
Lung Fibroblasts ◽  
Collagen Secretion ◽  
Human Fetal Lung

Glucocorticoid regulation of epithelial sodium channel genes in human fetal lung

1997 ◽  
Vol 273 (1) ◽  
pp. L227-L233 ◽  
Author(s):  
V. C. Venkatesh ◽  
H. D. Katzberg
Keyword(s):  
Rna Polymerase Ii ◽  
Actinomycin D ◽  
Critical Role ◽  
Fetal Lung ◽  
Explant Culture ◽  
Late Gestation ◽  
Na Channel ◽  
Second Trimester ◽  
Cyclic Monophosphate ◽  
Human Fetal Lung

Pulmonary epithelial Na+ channels (ENaC), composed of three distinct subunits (alpha, beta, and gamma), play a critical role in the regulation of fluid reabsorption from airspaces of late-gestation fetal lung. We studied the expression of ENaC subunit genes in cultured human fetal lung. All three mRNAs were expressed at low levels in second trimester lung (13-32% of adult values at 24 wk gestation). There was a spontaneous increase of approximately threefold over preculture values of all three subunits within 24 h of explant culture in serum-free Waymouth's medium. Dexamethasone (Dex) induced all three mRNAs by two- to threefold. Maximal induction was noted by 8 h with 30-100 nM Dex and half-maximal stimulation with 3-10 nM Dex. Cycloheximide decreased basal expression of all three subunits by 8 h but did not alter the response to Dex. Actinomycin D and 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB), inhibitors of RNA polymerase II, decreased the basal and the Dex-induced expression of all three subunits with a more marked effect on human hENaC-gamma than on hENaC-alpha or hENaC-beta. Under conditions where transcription was blocked by actinomycin D or DRB, Dex did not alter the stability of the three mRNAs. Triiodothyronine (T3) at low (2 nM) or high (100 nM) concentrations had no effect on the expression of the three subunits in the presence or absence of low (10 nM) or high (100 nM) concentrations of Dex for 8 or 24 h. Similarly, 8-bromoadenosine 3',5'-cyclic monophosphate (2 microM) had no effect on basal or Dex-induced increase in the three subunits. We conclude that the three Na+ channel subunit genes are expressed in second trimester human fetal lung and are coordinately upregulated by glucocorticoid hormones but not by T3 or adenosine 3',5'-cyclic monophosphate. Glucocorticoid induction is receptor mediated, is primarily transcriptional, and does not require the induction of an intermediate protein for transcriptional enhancement. We speculate that induction of lung ENaC may contribute to the beneficial effects of antenatal glucocorticoids in premature babies.

Fabrication and Cytotoxicity Studies of the TiO2 Doped with Copper-Based Nanocomposite Particles

2011 ◽  
Vol 695 ◽  
pp. 393-396 ◽  
Author(s):  
Chang Mao Hung
Keyword(s):  
Fetal Lung ◽  
Copper Nitrate ◽  
Tissue Cell ◽  
Nanocomposite Particles ◽  
Toxicological Effect ◽  
Cytotoxicity Studies ◽  
Cultured Human Cells ◽  
Cytotoxicity Assessment ◽  
Human Fetal Lung ◽  
Dispersion Character

Since the growing interest in the manufacture and environmental applications of nanocomposites consisting of CuO and TiO2nanoparticles (NPs), related toxicological effect and interaction with cellular structures for these newly developed materials are still unknown. Recent literature reveals that nanosized CuO and TiO2particles have cytotoxicity risks and ability to cause oxidative stress on health. This work considers the CuO doped TiO2nanocomposite particles were synthesized via a coprecipitation method with aqueous solutions as precursors of copper nitrateand titanium dioxide. Moreover, the nanocomposite particles were characterized using TGA-DTA, UV-Vis and TEM measurements. The calcination temperature was selected at 873 K. The nanocomposite particles were characterized by TEM, as the primary particles, aggregates ranged from 30 to 100 nm and have a good dispersion character. Cell cytotoxicity assessment and the percentage cell survival was determined by using 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethoxyphenol)-2-(4-sulfophenyl)-2H-tetrazoli um (MTS) assay on human fetal lung tissue cell (MRC-5). The experimental results show that the CuO doped TiO2nanocomposite particles cause potential cytotoxicity effect in cultured human cells.

scholarly journals MORPHOGENETIC ASPECTS OF MULTILAYERING IN PETRI DISH CULTURES OF HUMAN FETAL LUNG FIBROBLASTS

1969 ◽  
Vol 41 (1) ◽  
pp. 298-311 ◽  
Author(s):  
Tom Elsdale ◽  
Robert Foley
Keyword(s):  
Extracellular Matrix ◽  
Human Lung ◽  
Petri Dish ◽  
Fetal Lung ◽  
Single Species ◽  
Lung Fibroblasts ◽  
Human Lung Fibroblasts ◽  
Spontaneous Aggregation ◽  
Control Hypothesis ◽  
Human Fetal Lung

Randomly seeded Petri dish cultures of embryonic human lung fibroblasts generate, in the course of their growth, highly ordered cellular arrangements. Thick, bilaterally symmetrical ridges with an axial polarity and an orthogonal, multilayered internal organization are observed within stationary cultures. The generation of these structures has been investigated. Ridges result from the spontaneous aggregation of cells in postconfluent cultures brought about by directed cell movements. These movements are promoted by the localized production of extracellular matrix sheets containing collagen, which provide new substrates for cellular colonization. Cells that have colonized one matrix substrate may secrete another above themselves, which will in turn be colonized. By a continuation of this cycle, thick stacks consisting of alternate layers of cells and matrix are produced to yield the observed aggregations. The distribution and shape of ridges in a culture imply that matrix substrates are confined to specific locations. The suggested control hypothesis assumes that all the cells in fibroblast cultures are potential producers of a single species of matrix. The serviceability of this matrix as a substrate for cellular colonization, however, is destroyed if the producer cells are motile. Matrix substrates, therefore, are only made by nonmotile cells.

Comparison of WI-38, MRC-5, and IMR-90 cell strains for isolation of viruses from clinical specimens

1978 ◽  
Vol 7 (4) ◽  
pp. 368-371
Author(s):  
H M Friedman ◽  
C Koropchak
Keyword(s):  
Tissue Culture ◽  
Life Span ◽  
Fetal Lung ◽  
Cell Strain ◽  
Viral Isolation ◽  
Clinical Specimens ◽  
Cell Strains ◽  
Viral Diagnosis ◽  
Human Fetal Lung ◽  
Speed Of Onset

With the diminishing supply of the human fetal lung WI-38 cell strain, a replacement for viral isolation is needed. Two candidates are the human fetal lung strains MRC-5 and IMR-90. A comparison of WI-38, MRC-5, and IMR-90 was performed to evaluate efficiency and speed of viral isolation, clarity of cytophatic effect, and ease of growing the cells. The inocula were clinical specimens rather than tissue culture-adapted isolates. Frozen samples of 46 specimens that had previously yielded an isolate on WI-38 were thawed and inoculated onto WI-38, MRC-5, and IMR-90 cells. In addition, 95 freshly taken clinical specimens uf undetermined infectivity were inoculated onto the cell strains. Viral recovery rates were similar on all three strains, as were the appearance and speed of onset of the cytophatic effect. MRC-5 and WI-38 cells remained healthy until generation 36, whereas IMR-90 cells went into crisis by generation 20. The longer life span of the MRC-5 cells makes them more suitable than IMR-90 cells to replace the WI-38 strain for routine use in viral diagnosis.

Oxygen modulates the differentiation of human fetal lung in vitro and its responsiveness to cAMP

1993 ◽  
Vol 264 (5) ◽  
pp. L465-L474 ◽  
Author(s):  
M. J. Acarregui ◽  
J. M. Snyder ◽  
C. R. Mendelson
Keyword(s):  
Gene Expression ◽  
Atmospheric Oxygen ◽  
Protein A ◽  
Morphological Differentiation ◽  
Fetal Lung ◽  
Morphological Development ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Human Fetal Lung

Previously, it was found that lung explants from mid-trimester human abortuses differentiate spontaneously in organ culture in serum-free defined medium in an atmosphere of 95% air-5% CO2. Dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP) treatment of human fetal lung in culture increases the rate of morphological differentiation and enhances expression of the surfactant protein A (SP-A) gene. To begin to define the factors responsible for this accelerated in vitro differentiation, we analyzed the effects of atmospheric oxygen on the morphological and biochemical development of human fetal lung in culture and on responsiveness of the cultured tissue to DBcAMP. We found that when lung explants were maintained in an atmosphere containing 1% oxygen they failed to differentiate spontaneously and no induction of SP-A gene expression was apparent. Furthermore, at 1% oxygen, DBcAMP had no effect to stimulate morphological differentiation or SP-A gene expression. When lung tissues that had been maintained for 5 days in 1% oxygen were transferred to an environment containing 20% oxygen, there was rapid morphological development and induction of SP-A gene expression. The effects on morphological development were manifest within 24 h of transfer to the 20% oxygen environment; within 72 h, a marked stimulatory effect of DBcAMP on SP-A gene expression also was observed. Our findings further suggest that the effects of oxygen on the levels of SP-A and SP-A mRNA are concentration dependent. Interestingly, the inductive effects of DBcAMP on SP-A gene expression were apparent only at oxygen concentrations > or = 10%. Morphological differentiation of the cultured human fetal lung tissue also was influenced by oxygen in a concentration-dependent manner. These findings suggest that oxygen plays an important permissive role in the spontaneous differentiation of human fetal lung in vitro.

Differentiation of Type II Cells during Explant Culture of Human Fetal Lung Is Accelerated by Endogenous Prostanoids and Adenosine 3′,5′-Monophosphate*

Endocrinology ◽  
1991 ◽  
Vol 128 (6) ◽  
pp. 2916-2924 ◽  
Author(s):  
PHILIP L. BALLARD ◽  
LINDA W. GONZALES ◽  
MARY C. WILLIAMS ◽  
JAMES M. ROBERTS ◽  
MARK M. JACOBS
Keyword(s):  
Fetal Lung ◽  
Explant Culture ◽  
Type Ii Cells ◽  
Type Ii ◽  
Human Fetal Lung
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