Rapid isoenzyme analysis of cell cultures by agarose electrophoresis II. Intraspecies identification of human cell lines

1983 ◽  
Vol 8 (3) ◽  
pp. 125-129 ◽  
Author(s):  
Mark J. Ottenbreit ◽  
David M. Halton ◽  
Ward D. Peterson
Keyword(s):  
Cell Lines ◽  
Cell Cultures ◽  
Human Cell ◽  
Human Cell Lines ◽  
Isoenzyme Analysis ◽  
Agarose Electrophoresis

scholarly journals Effects of reverse transcriptase inhibitors on telomere length and telomerase activity in two immortalized human cell lines.

1996 ◽  
Vol 16 (1) ◽  
pp. 53-65 ◽  
Author(s):  
C Strahl ◽  
E H Blackburn
Keyword(s):  
Reverse Transcriptase ◽  
B Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Cell Cultures ◽  
Human Cell ◽  
Growth Rates ◽  
Telomerase Activity ◽  
Human Cell Lines ◽  
Telomere Shortening

The ribonucleoprotein telomerase, a specialized cellular reverse transcriptase, synthesizes one strand of the telomeric DNA of eukaryotes. We analyzed telomere maintenance in two immortalized human cell lines: the B-cell line JY616 and the T-cell line Jurkat E6-1, and determined whether known inhibitors of retroviral reverse transcriptases could perturb telomere lengths and growth rates of these cells in culture. Dideoxyguanosine (ddG) caused reproducible, progressive telomere shortening over several weeks of passaging, after which the telomeres stabilized and remained short. However, the prolonged passaging in ddG caused no observable effects on cell population doubling rates or morphology. Azidothymidine (AZT) caused progressive telomere shortening in some but not all T- and B-cell cultures. Telomerase activity was present in both cell lines and was inhibited in vitro by ddGTP and AZT triphosphate. Prolonged passaging in arabinofuranyl-guanosine, dideoxyinosine (ddI), dideoxyadenosine (ddA), didehydrothymidine (d4T), or phosphonoformic acid (foscarnet) did not cause reproducible telomere shortening or decreased cell growth rates or viabilities. Combining AZT, foscarnet, and/or arabinofuranyl-guanosine with ddG did not significantly augment the effects of ddG alone. Strikingly, with or without inhibitors, telomere lengths were often highly unstable in both cell lines and varied between parallel cell cultures. We propose that telomere lengths in these T- and B-cell lines are determined by both telomerase and telomerase-independent mechanisms.

scholarly journals PROPERTIES OF PLASMINOGEN ACTIVATORS FORMED BY NEOPLASTIC HUMAN CELL CULTURES

1974 ◽  
Vol 139 (5) ◽  
pp. 1317-1328 ◽  
Author(s):  
Daniel B. Rifkin ◽  
John N. Loeb ◽  
George Moore ◽  
E. Reich
Keyword(s):  
Plasminogen Activator ◽  
Cell Lines ◽  
Cell Cultures ◽  
Human Cell ◽  
Serine Proteases ◽  
Plasminogen Activators ◽  
Human Embryos ◽  
Human Cell Lines ◽  
Human Sera ◽  
A Minor

A series of human cell lines has been examined for fibrinolysis in culture. The sera that are activating for fibrinolysis by human cells are mouse, monkey, human, horse, and bovine. Individual human sera show considerable variation in the ability to activate fibrinolysis. In common with other neoplastic or transformed mammalian and avian cell cultures, human cell lines of neoplastic origin produce substantial amounts of plasminogen activator. Several cultures of nonmalignant origin also produce plasminogen activator, whereas cultures obtained from trypsinized human embryos, or from human embryonic skin do not. The human melanoma plasminogen activators are of two kinds: a major component with a mol wt of 50,000, and a minor species with a mol wt of approximately 60,000. Both are DFP sensitive, serine proteases.

Investigations of the influence of microgravity on the state of human cell lines

2004 ◽  
Vol 10 (5-6) ◽  
pp. 226-228
Author(s):  
L.M. Nosach ◽  
◽  
O.Yu. Povnitsa ◽  
V.L. Zhovnovata ◽  
◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Cell ◽  
The State ◽  
Human Cell Lines

scholarly journals Dicalcin suppresses in vitro trophoblast attachment in human cell lines

2021 ◽  
Vol 570 ◽  
pp. 206-213
Author(s):  
Ryohei Saito ◽  
Hiromasa Satoh ◽  
Kayo Aoba ◽  
Hajime Hirasawa ◽  
Naofumi Miwa
Keyword(s):  
Cell Lines ◽  
Human Cell ◽  
Human Cell Lines

scholarly journals Differential Effects of Cyanidin and Cyanidin-3-glucoside on Human Cell Lines

2011 ◽  
Vol 17 (6) ◽  
pp. 515-521 ◽  
Author(s):  
Masayuki TAKEUCHI ◽  
Katsuki OHTANI ◽  
Yanju MA ◽  
Sanae KATO ◽  
Shingo SEMBA ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Cell ◽  
Human Cell Lines ◽  
Differential Effects
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