In vitro toxicity screening tests: A comparison of activation systems and treatment protocols among different laboratories

1983 ◽  
Vol 8 (1) ◽  
pp. 7-11 ◽  
Author(s):  
Sabine H. H. Swierenga ◽  
David E. Amacher ◽  
Robert T. Christian ◽  
Joanne T. Emerman ◽  
Linda B. Jacobsen ◽  
...  
Keyword(s):  
Screening Tests ◽  
In Vitro Toxicity ◽  
Toxicity Screening ◽  
Treatment Protocols

Some experimental aspects of an in vitro toxicity screening in developing new pharmaceuticals

2017 ◽  
Vol 280 ◽  
pp. S267
Author(s):  
Alla Voronina ◽  
Zinaida Klestova ◽  
Viktor Tashuta
Keyword(s):  
In Vitro Toxicity ◽  
Toxicity Screening

In Vitro Screening for Hepatotoxicity of Xenobiotics using Isolated Hepatocytes

1988 ◽  
Vol 15 (3) ◽  
pp. 201-207
Author(s):  
Karel Lavrijsen ◽  
Derk Van Dijck ◽  
Jos Van Houdt ◽  
Ingrid Van Pelt ◽  
Willem Meuldermans ◽  
...  
Keyword(s):  
Culture Medium ◽  
Isolated Hepatocytes ◽  
Cell Number ◽  
Culture Conditions ◽  
Male Rats ◽  
In Vitro Toxicity ◽  
Toxicity Screening ◽  
Ldh Release

Isolated hepatocytes from male rats were cultured in primary cell culture in 24-well dishes for 20–24 hours in the presence of the various test compounds. Cytotoxicity at the end of the culture period was evaluated by the determination of LDH-release into the culture medium or by quantification of DNA content as a measure of the cell number in each well. Toxic xenobiotics, such as chlorpromazine, were able to induce LDH-release at up to 5 times the background levels at concentrations of 10-4–10-5 M. For most compounds, the increase in LDH-release was closely related to a decrease in cell number, as measured by the DNA method. Two culture conditions which might influence the cytotoxic response were investigated. Prolongation of the culture time increased the toxicity of some compounds, e.g. sodium dodecylsulphate, whereas for other compounds, e.g. amitriptyline, no change was noted. Addition of serum albumin and α1-acid glycoprotein decreased the toxicity of chlorpromazine to isolated hepatocytes. These results indicate that isolated hepatocytes might represent a useful in vitro toxicity screening system, and that careful standardisation of the culture conditions is necessary.

Human iPSC-derived retinal organoid model for in vitro toxicity screening

2021 ◽  
Vol 350 ◽  
pp. S45
Author(s):  
V. Chichagova ◽  
B. Dorgau ◽  
C. de Santis ◽  
M. Georgiou ◽  
M Carter ◽  
...  
Keyword(s):  
In Vitro Toxicity ◽  
Toxicity Screening ◽  
Human Ipsc

scholarly journals Engineered nanoparticle bio-conjugates toxicity screening: The xCELLigence cells viability impact

Bioimpacts ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 195-203
Author(s):  
Clarence S Yah ◽  
Geoffrey S. Simate
Keyword(s):  
Animal Model ◽  
Real Time ◽  
In Vitro Toxicity ◽  
Toxicity Screening ◽  
Limited Information ◽  
Size Estimation ◽  
Model Studies ◽  
Engineered Nanoparticle

Introduction: The vast diverse products and applications of engineered nanoparticle bio-conjugates (ENPBCs) are increasing, and thus flooding the-markets. However, the data to support risk estimates of ENPBC are limited. While it is important to assess the potential benefits, acceptability and uptake, it is equally important to understand where ENPBCs safety is and how to expand and affirm consumer security concerns. Methods: Online articles were extracted from 2013 to 2016 that pragmatically used xCELLigence real-time cell analysis (RTCA) technology to describe the in-vitro toxicity of ENPBCs. The xCELLigence is a +noninvasive in vitro toxicity monitoring process that mimics exact continuous cellular bio-responses in real-time settings. On the other hand, articles were also extracted from 2008 to 2016 describing the in vivo animal models toxicity of ENPBCs with regards to safety outcomes. Results: Out of 32 of the 121 (26.4%) articles identified from the literature, 23 (71.9%) met the in-vitro xCELLigence and 9(28.1%) complied with the in vivo animal model toxicity inclusion criteria. Of the 23 articles, 4 of them (17.4%) had no size estimation of ENPBCs. The xCELLigence technology provided information on cell interactions, viability, and proliferation process. Eighty-three (19/23) of the in vitro xCELLigence technology studies described ENPBCs as nontoxic or partially nontoxic materials. The in vivo animal model provided further toxicity information where 1(1/9) of the in vivo animal model studies indicated potential animal toxicity while the remaining results recommended ENPPCs as potential candidates for drug therapy though with limited information on toxicity. Conclusion: The results showed that the bioimpacts of ENPBCs either at the in vitro or at in vivo animal model levels are still limited due to insufficient information and data. To keep pace with ENPBCs biomedical products and applications, in vitro, in vivo assays, clinical trials and long-term impacts are needed to validate their usability and uptake. Besides, more real-time ENPBCs-cell impact analyses using xCELLigence are needed to provide significant data and information for further in vivo testing.

IN VITRO TOXICITY SCREENING OF DIELECTRIC GASES USING MAMMALIAN CELLS

2013 ◽  
pp. 420-426 ◽  
Author(s):  
G.D. Griffin ◽  
B.A. Owen ◽  
C.E. Easterly ◽  
P.J. Walsh
Keyword(s):  
Mammalian Cells ◽  
In Vitro Toxicity ◽  
Toxicity Screening
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