Dedicated image analysis techniques for three-dimensional reconstruction from serial sections in electron microscopy

1991 ◽  
Vol 4 (4) ◽  
pp. 263-270 ◽  
Author(s):  
Ph. Gremillet ◽  
M. Jourlin ◽  
Cph Bron ◽  
J. Schüpbach ◽  
H. P. Gautschi ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Image Analysis ◽  
Three Dimensional ◽  
Serial Sections ◽  
Three Dimensional Reconstruction ◽  
Analysis Techniques ◽  
Dimensional Reconstruction ◽  
Image Analysis Techniques

Three-Dimensional Reconstruction of Native Androctonus Australis Hemocyanin

1990 ◽  
Vol 48 (1) ◽  
pp. 452-453
Author(s):  
Nicolas Boisset ◽  
Jean-Christophe Taveau ◽  
Jean Lamy ◽  
Terence Wagenknecht ◽  
Michael Radermacher ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Solid Body ◽  
Body Surface ◽  
Three Dimensional ◽  
Staining Technique ◽  
Three Dimensional Reconstruction ◽  
Dimensional Reconstruction ◽  
View Reconstruction ◽  
Surface Representations ◽  
Top View

Hemocyanin, the respiratory pigment of the scorpion Androctonus australis is composed of 24 kidney shaped subunits. A model of architecture supported by many indirect arguments has been deduced from electron microscopy (EM) and immuno-EM. To ascertain, the disposition of the subunits within the oligomer, the 24mer was submitted to three-dimensional reconstruction by the method of single-exposure random-conical tilt series.A sample of native hemocyanin, prepared with the double layer negative staining technique, was observed by transmisson electron microscopy under low-dose conditions. Six 3D-reconstructions were carried out indenpendently from top, side and 45°views. The results are composed of solid-body surface representations, and slices extracted from the reconstruction volume.The main two characters of the molecule previously reported by Van Heel and Frank, were constantly found in the solid-body surface representations. These features are the presence of two different faces called flip and flop and a rocking of the molecule around an axis passing through diagonnally opposed hexamers. Furthermore, in the solid-body surface of the top view reconstruction, the positions and orientations of the bridges connecting the half molecules were found in excellent agreement with those predicted by the model.

Three-Dimensional Reconstruction From Serial Sections For Medical Applications

1981 ◽  
Author(s):  
Prakairut N. Cook ◽  
Solomon Batnitzky ◽  
Kyo R. Lee ◽  
Errol Levine ◽  
Hilton I. Price ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Medical Applications ◽  
Serial Sections ◽  
Three Dimensional Reconstruction ◽  
Dimensional Reconstruction
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