γ-Glutamyl transpeptidase-cellular expression in populations of normal human mononuclear cells and patients suffering from leukemias

1995 ◽  
Vol 70 (5) ◽  
pp. 237-242 ◽  
Author(s):  
M. Täger ◽  
A. Ittenson ◽  
A. Franke ◽  
A. Frey ◽  
H. G. Gassen ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Human Mononuclear Cells ◽  
Cellular Expression ◽  
Normal Human ◽  
Glutamyl Transpeptidase

?-Glutamyl transpeptidase - cellular expression in populations of normal human mononuclear cells and patients suffering from leukemias

1995 ◽  
Vol 70 (5) ◽  
pp. 237-242 ◽  
Author(s):  
M. T�ger ◽  
A. Ittenson ◽  
A. Franke ◽  
A. Frey ◽  
H. G. Gassen ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Human Mononuclear Cells ◽  
Cellular Expression ◽  
Normal Human ◽  
Glutamyl Transpeptidase

scholarly journals Tuftsin induces tissue factor-like activity in human mononuclear cells and in monocytic cell lines

Blood ◽  
1990 ◽  
Vol 76 (4) ◽  
pp. 814-819 ◽  
Author(s):  
A Kornberg ◽  
R Catane ◽  
S Peller ◽  
S Kaufman ◽  
M Fridkin
Keyword(s):  
Tissue Factor ◽  
Cell Lines ◽  
Mononuclear Cells ◽  
Monocytic Cell ◽  
Human Mononuclear Cells ◽  
Monocytes And Macrophages ◽  
Normal Human ◽  
Natural Stimulator ◽  
Coagulation Pathway ◽  
Lymphoid Cell Lines

Abstract Normal human monocytes and macrophages generate potent procoagulant activity (PCA) resembling tissue factor (TF) in response to various stimuli. In this study we show that tuftsin, a natural stimulator of many functions of monocytes and macrophages, also stimulates a potent PCA in mixed mononuclear cells and monocytes, and a mild PCA in lymphocytes and cell lines of monocytic origin (U937 and THP). No activity was generated by several lymphoid cell lines and HL-60 cells. The PCA resembled TF in that it accelerated clotting through the extrinsic coagulation pathway and was inhibited by concanavalin-A and by monoclonal anti-TF antibodies. The induction of TF-like activity by tuftsin was dose- and time-dependent. It was located in the cell membrane and did not require T cells for expression. Generation of TF- like activity was prevented by actinomycin D, while cytarabine had no effect on this process, suggesting that expression of the activity depends on protein synthesis. Studies with various tuftsin analogs suggest that tuftsin stimulates generation of TF-like activity, as well as other functions of monocytes via the same receptors. The results with the monocytic cell lines show that tuftsin affects mainly mature cells. The induction of TF-like activity in mononuclear cells by tuftsin constitutes an important link between mononuclear cells and the immune and coagulation systems. It may play a major role in the pathogenesis of thromboembolism and fibrin deposition in various inflammatory and immunologic disorders.

scholarly journals Effect of arsenic, cadmium and lead on the induction of apoptosis of normal human mononuclear cells

2002 ◽  
Vol 129 (1) ◽  
pp. 69-77 ◽  
Author(s):  
H. DE LA FUENTE ◽  
D. PORTALES-PÉREZ ◽  
L. BARANDA ◽  
F. DÍAZ-BARRIGA ◽  
V. SAAVEDRA-ALANÍS ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Human Mononuclear Cells ◽  
Normal Human ◽  
Induction Of Apoptosis ◽  
Cadmium And Lead

scholarly journals Protective Effect of Tetrandrine on Normal Human Mononuclear Cells against Ionizing Irradiation.

1997 ◽  
Vol 20 (11) ◽  
pp. 1160-1164 ◽  
Author(s):  
Yu-Jen CHEN ◽  
Mang-Lin TU ◽  
Hsiang-Chi KUO ◽  
Kuo-Hua CHANG ◽  
Yuen-Liang LAI ◽  
...  
Keyword(s):  
Protective Effect ◽  
Mononuclear Cells ◽  
Ionizing Irradiation ◽  
Human Mononuclear Cells ◽  
Normal Human

scholarly journals Human mononuclear cells contain an endoglycosidase specific for heparan sulphate glycosaminoglycan demonstrable with the use of a specific solid-phase metabolically radiolabelled substrate

1989 ◽  
Vol 264 (3) ◽  
pp. 777-783 ◽  
Author(s):  
R F Sewell ◽  
P E C Brenchley ◽  
N P Mallick
Keyword(s):  
Affinity Chromatography ◽  
Mononuclear Cells ◽  
Solid Phase ◽  
Chondroitin Sulphate ◽  
Heparan Sulphate ◽  
Human Spleen ◽  
Human Mononuclear Cells ◽  
Normal Human ◽  
Sepharose 4B ◽  
Heparin Affinity

Xyloside-initiated 35SO4(2-)-labelled glycosaminoglycans were isolated from the medium of cultured bovine glomeruli and covalently coupled to Sepharose 4B to construct a solid-phase substrate suitable for the detection of endoglycosidases. The substrate is rendered specific for heparitinase by prior digestion with chondroitin sulphate ABC lyase and is insensitive to proteinase, neuraminidase and hyaluronidase. Normal human mononuclear cells are shown to contain a heparitinase. This enzyme appears to be cell-associated and can be partially purified from human spleen by heparin affinity chromatography.

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