Antitumor effect of combination of murine recombinant interferon β, murine recombinant interferon γ and human recombinant interleukin-2 in MethA-bearing mice

1990 ◽  
Vol 32 (2) ◽  
pp. 88-94 ◽  
Author(s):  
Tohru Itoh ◽  
Yuh Sakata ◽  
Yutaka Yoshida ◽  
Kenichi Tsushima ◽  
Hidekazu Suzuki ◽  
...  
Keyword(s):  
Antitumor Effect ◽  
Interleukin 2 ◽  
Interferon Γ ◽  
Interferon Β ◽  
Recombinant Interferon ◽  
Recombinant Interleukin 2

scholarly journals Interleukin-6 (B-cell stimulatory factor 2)-dependent growth of a Lennert's lymphoma-derived T-cell line (KT-3)

Blood ◽  
1988 ◽  
Vol 72 (5) ◽  
pp. 1826-1828
Author(s):  
S Shimizu ◽  
T Hirano ◽  
R Yoshioka ◽  
S Sugai ◽  
T Matsuda ◽  
...  
Keyword(s):  
Cell Line ◽  
Interleukin 2 ◽  
Recombinant Interferon ◽  
Plot Analysis ◽  
Recombinant Interleukin 2 ◽  
T Lymphoma ◽  
Dependent Growth ◽  
Stimulatory Factor ◽  
Recombinant Interferon Alpha ◽  
Necrosis Factor

A T lymphoma cell line (KT-3) established from a patient with Lennert's lymphoma showed macrophage-dependent growth. Macrophage-derived factors were able to replace the macrophage functions. Experiments using a variety of cytokines demonstrated that KT-3 proliferated in response to recombinant interleukin-2 (rIL-2), rIL-4, or rIL-6 but did not proliferate in response to rIL-1 alpha, rIL-1 beta, rIL-3, recombinant granulocyte colony-stimulating factor (rG-CSF), rGM-CSF, recombinant interferon-alpha (rIFN-alpha), rIFN-gamma, recombinant tumor necrosis factor (rTNF-alpha), or native IFN-beta. Polyclonal rabbit anti-IL-6 antibody almost completely neutralized the activities of macrophage- derived factors or IL-6 but not IL-2 or IL-4. Scatchard plot analysis demonstrated that KT-3 cells indeed express IL-6 receptors. The results indicate that the macrophage-derived factor that supports the growth of KT-3 is IL-6 and suggest that macrophage-derived IL-6 may play an important role in the histopathogenesis of Lennert's lymphoma.

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