A quantitative histochemical study of 5′-nucleotidase activity in rat liver using the lead salt method and polyvinyl alcohol

1988 ◽  
Vol 20 (4) ◽  
pp. 207-214 ◽  
Author(s):  
Wilma M. Frederiks ◽  
Frans Marx
Keyword(s):  
Polyvinyl Alcohol ◽  
Rat Liver ◽  
Histochemical Study ◽  
Lead Salt ◽  
Nucleotidase Activity

A quantitative histochemical study of 5′nucleotidase activity in rat liver after ischaemia

1988 ◽  
Vol 154 (3) ◽  
pp. 277-286 ◽  
Author(s):  
Wilma M. Frederiks ◽  
Frans Marx ◽  
Galja L. Myagkaya
Keyword(s):  
Rat Liver ◽  
Histochemical Study ◽  
Nucleotidase Activity

A Histochemical Study of Embryonic Rat Liver in Avitaminosis E

1967 ◽  
Vol 91 (2) ◽  
pp. 159-173 ◽  
Author(s):  
Dorothy Wei King ◽  
Kusum Verma
Keyword(s):  
Rat Liver ◽  
Histochemical Study ◽  
Avitaminosis E

scholarly journals A quantitative histochemical study of xanthine oxidase activity in rat liver using the cerium capture method in the presence of polyvinyl alcohol.

1994 ◽  
Vol 42 (8) ◽  
pp. 1091-1096 ◽  
Author(s):  
W M Frederiks ◽  
K S Bosch ◽  
R J Van den Munckhof ◽  
C J Van Noorden
Keyword(s):  
Polyvinyl Alcohol ◽  
Xanthine Oxidase ◽  
Rat Liver ◽  
Incubation Medium ◽  
Oxidase Activity ◽  
Specific Inhibitor ◽  
Semipermeable Membrane ◽  
Final Reaction Product ◽  
Final Reaction ◽  
Xanthine Oxidase Activity

A recently developed histochemical technique to demonstrate xanthine oxidase activity in milk globules of bovine mammary gland and in epithelial cells of rat small intestine using cerium ions and a semipermeable membrane was slightly modified. The semipermeable membrane method was replaced by the addition of 10% (w/v) polyvinyl alcohol to the incubation medium. This technically more simple procedure enabled detection of xanthine oxidase activity in unfixed cryostat sections of rat liver. Both methods gave qualitatively and quantitatively similar results. Activity was found in sinusoidal cells and in liver parenchymal cells, with 50% higher activity in pericentral than in periportal areas. The specificity of the reaction was proven by the generation of only small amounts of final reaction product on incubation either in the absence of the substrates hypoxanthine or oxygen or in the presence of hypoxanthine and allopurinol. Allopurinol is a specific inhibitor of xanthine oxidase activity. The amount of final reaction product, as measured cytophotometrically in rat liver, increased linearly with incubation time (15-90 min) and with section thickness (up to 12 microns). By varying the hypoxanthine concentrations, a Km value of 0.05 mM was found. Addition of dithiothreitol to the incubation medium reduced the amount of final reaction product by 85%, which was caused by conversion of reversible xanthine oxidase into xanthine dehydrogenase. This histochemical method can be used for quantitative analysis of in situ xanthine oxidase activity.

ATPase and 5?-nucleotidase activity of rat liver nuclear matrix

1981 ◽  
Vol 91 (4) ◽  
pp. 531-533
Author(s):  
I. B. Bukhvalov ◽  
V. V. Delektorskaya ◽  
K. A. Perevoshchikova ◽  
I. B. Zbarskii ◽  
N. T. Raikhlin
Keyword(s):  
Rat Liver ◽  
Nuclear Matrix ◽  
Nucleotidase Activity

scholarly journals Intracellular Distribution of 5' Nucleotidase in Rat Liver

1958 ◽  
Vol 4 (4) ◽  
pp. 373-376 ◽  
Author(s):  
Gaston de Lamirande ◽  
Claude Allard ◽  
Antonio Cantero
Keyword(s):  
Enzymatic Activity ◽  
Rat Liver ◽  
Biochemical Analysis ◽  
Inorganic Phosphorus ◽  
Intracellular Distribution ◽  
Differential Centrifugation ◽  
Nucleotidase Activity ◽  
Rat Liver Cells ◽  
Cell Fractions

The intracellular distribution of 5' nucleotidase was investigated in rat liver by biochemical analysis of cell fractions obtained by differential centrifugation. The enzymatic activity was measured by determination of the inorganic phosphorus liberated from 5' nucleotides. The 5' nucleotidase activity was mainly found in the nuclear and microsomal fractions. An attempt to extract the enzyme from these fractions with Mg++ ion solutions was unsuccessful. It is concluded that 5' nucleotidase is actually present in the nuclear and microsomal fractions of rat liver cells.

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