Renal cell carcinoma treated by vaccines for active specific immunotherapy: Correlation of survival with skin testing by autologous tumor cells

1990 ◽  
Vol 32 (1) ◽  
pp. 62-66 ◽  
Author(s):  
Craig S. McCune ◽  
Robert W. O'Donnell ◽  
Diana M. Marquis ◽  
Deepak M. Sahasrabudhe
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Tumor Cells ◽  
Renal Cell ◽  
Specific Immunotherapy ◽  
Skin Testing ◽  
Autologous Tumor ◽  
Active Specific Immunotherapy

Lysis of Autologous Tumor Cells by Peripheral Blood Lymphocytes Treated with Interleukin 2 in Patients with Renal Cell Carcinoma

1987 ◽  
Vol 137 (4) ◽  
pp. 641-647 ◽  
Author(s):  
Etsuji Nakano ◽  
Yasuharu Tada ◽  
Yasuji Ichikawa ◽  
Hideki Fujioka ◽  
Minoru Matsuda ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Tumor Cells ◽  
Peripheral Blood ◽  
Renal Cell ◽  
Interleukin 2 ◽  
Peripheral Blood Lymphocytes ◽  
Autologous Tumor ◽  
Blood Lymphocytes

scholarly journals Autologous tumor-specific cytotoxic T lymphocytes in the infiltrate of human metastatic melanomas. Activation by interleukin 2 and autologous tumor cells, and involvement of the T cell receptor.

1988 ◽  
Vol 168 (4) ◽  
pp. 1419-1441 ◽  
Author(s):  
K Itoh ◽  
C D Platsoucas ◽  
C M Balch
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Nk Cells ◽  
Metastatic Melanoma ◽  
Tumor Cells ◽  
Renal Cell ◽  
Cell Receptor ◽  
Autologous Tumor ◽  
Alpha Beta ◽  
Ctl Activity

TIL from metastatic melanoma proliferated by greater than 1,000-fold (840-3,675, mean 1,543) after 6 wk in culture of mixtures of TIL and tumor cells with rIL-2 alone. Cytolysis was restricted to autologous tumor cells. CD8+ T cells were the predominant population of TIL before and after expansion, and were primarily responsible for autologous tumor-specific CTL activity. No other rIL-2-activated lymphocytes from peripheral blood, lymph nodes with melanoma metastasis, or TIL from sarcoma or renal cell carcinoma had autologous tumor-specific CTL activity. There were few or no CD16+ NK cells in TIL from metastatic melanoma before or after incubation with rIL-2, respectively. However, TIL from sarcoma or renal cell carcinoma contained a substantial proportion of CD3-CD16+ NK cells, which increased in number in culture with rIL-2. Purified CD16+ NK cells as well as CD3+CD16- T cells from rIL-2-activated TIL of renal cell carcinoma displayed MHC-nonrestricted cytotoxicity. At the clonal level as determined by limiting dilution, 8 of 10 clones from melanoma TIL displayed cytotoxicity restricted to autologous tumor cells, while all 13 clones from renal cancer TIL equally lysed autologous and allogeneic tumor cells. Anti-T cell receptor (TCR)-alpha/beta(WT31) mAb as well as anti-CD3 mAb inhibited autologous melanoma cell-specific CTL activity mediated by rIL-2-activated TIL at the effector phase. These two mAbs also inhibited rIL-2-dependent proliferation of these TIL when added to the culture. Pretreatment of fresh melanoma cells with mAb to MHC antigens followed by washing inhibited specific CTL activity. These results suggest that both TCR-alpha/beta on effector TIL and MHC antigens on fresh tumor cells are involved in the specific immune-recognition. After reaching maximum propagation, TIL from metastatic melanoma responded poorly to rIL-2 alone. However, stimulation with fresh autologous melanoma cells restored both CTL activity and proliferation in response to rIL-2. The latter is associated with IL-2 receptor (Tac antigen) expression on the surface. These results indicate that TIL from metastatic melanomas may have unique characteristics different from lymphocytes obtained from the other sources, and may contain precursor CTL sensitized in vivo to autologous tumor cells, and thus can be propagated in larger numbers with rIL-2 alone while retaining autologous tumor-specific CTL activity.

Active Specific Immunotherapy of Renal Cell Carcinoma: Cellular and Humoral Immune Responses

1997 ◽  
Vol 12 (3) ◽  
pp. 157-165 ◽  
Author(s):  
Ulrich Zorn ◽  
Stefan Duensing ◽  
Frauke Langkopf ◽  
Gerasimos Anastassiou ◽  
Hartmut Kirchner ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Immune Responses ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Specific Immunotherapy ◽  
Humoral Immune ◽  
Humoral Immune Responses ◽  
Active Specific Immunotherapy
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