Human recombinant interleukin-6 enhances antibody-dependent cellular cytotoxicity of human tumor cells mediated by human peripheral blood mononuclear cells

1991 ◽  
Vol 34 (1) ◽  
pp. 9-16 ◽  
Author(s):  
K. Y. Tsang ◽  
M. D. Finch ◽  
F. J. Primus ◽  
J. Schlom
Keyword(s):  
Tumor Cells ◽  
Interleukin 6 ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Human Tumor ◽  
Cellular Cytotoxicity ◽  
Antibody Dependent Cellular Cytotoxicity ◽  
Human Tumor Cells ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

Antibody dependent cellular cytotoxicity activity of a novel anti-PD-L1 antibody, avelumab (MSB0010718C), on human tumor cells.

2015 ◽  
Vol 33 (15_suppl) ◽  
pp. 3038-3038 ◽  
Author(s):  
Kwong-Yok Tsang ◽  
Benjamin Boyerinas ◽  
Caroline Jochems ◽  
Massimo Fantini ◽  
Christopher Ryan Heery ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Human Tumor ◽  
Cellular Cytotoxicity ◽  
Antibody Dependent Cellular Cytotoxicity ◽  
Human Tumor Cells ◽  
Cytotoxicity Activity

scholarly journals Development of humanized bispecific antibodies reactive with cytotoxic lymphocytes and tumor cells overexpressing the HER2 protooncogene.

1992 ◽  
Vol 175 (1) ◽  
pp. 217-225 ◽  
Author(s):  
M R Shalaby ◽  
H M Shepard ◽  
L Presta ◽  
M L Rodrigues ◽  
P C Beverley ◽  
...  
Keyword(s):  
T Cells ◽  
Tumor Cells ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Human Tumor ◽  
Cytotoxic Activities ◽  
Cytotoxic Lymphocytes ◽  
Peripheral Blood Mononuclear ◽  
Antibody Molecule ◽  
Human T Cells

The HER2 protooncogene encodes a 185-kD transmembrane phosphoglycoproteins, human epidermal growth factor receptor 2 (p185HER2), whose amplified expression on the cell surface can lead to malignant transformation. Overexpression of HER2/p185HER2 is strongly correlated with progression of human ovarian and breast carcinomas. Recent studies have shown that human T cells can be targeted with bispecific antibody to react against human tumor cells in vitro. We have developed a bispecific F(ab')2 antibody molecule consisting of a humanized arm with a specificity to p185HER2 linked to another arm derived from a murine anti-CD3 monoclonal antibody that we have cloned from UCHT1 hybridoma. The antigen-binding loops for the anti-CD3 were installed in the context of human variable region framework residues, thus forming a fully humanized BsF(ab')2 fragment. Additional variants were produced by replacement of amino acid residues located in light chain complementarity determining region 2 and heavy chain framework region 3 of the humanized anti-CD3 arm. Flow cytometry analysis showed that the bispecific F(ab')2 molecules can bind specifically to cells overexpressing p185HER2 and to normal human peripheral blood mononuclear cells bearing the CD3 surface marker. In additional experiments, the presence of bispecific F(ab')2 caused up to fourfold enhancement in the cytotoxic activities of human T cells against tumor cells overexpressing p185HER2 as determined by a 51Cr release assay. These bispecific molecules have a potential use as therapeutic agents for the treatment of cancer.

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