A new estimate of sequence divergence of mitochondrial DNA using restriction endonuclease mappings

1979 ◽  
Vol 13 (4) ◽  
pp. 295-304 ◽  
Author(s):  
Norman Kaplan ◽  
Charles H. Langley
1992 ◽  
Vol 70 (11) ◽  
pp. 2161-2165 ◽  
Author(s):  
Seifu Seyoum ◽  
Irv Kornfield

Relationships among the seven recognized subspecies of the widespread African cichlid Oreochromis niloticus (L.) were investigated using restriction endonuclease analysis of mitochondrial DNA. Changes in nomenclature are based on estimates of sequence divergence and concordant results from phenetic, cladistic, and maximum likelihood analyses of molecular character sets. Oreochromis niloticus cancellatus and O. n. filoa are reassigned to O. cancellatus as O. c. cancellatus and O. c. filoa, respectively. The tilapiine fishes of Lake Tana, Ethiopia, previously assigned to O. n. cancellatus, are here described as Oreochromis niloticus tana subsp.nov. in recognition of their distinctive molecular phenotypes.


Genes ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 155
Author(s):  
Kefena Effa ◽  
Sonia Rosenbom ◽  
Jianlin Han ◽  
Tadelle Dessie ◽  
Albano Beja-Pereira

Matrilineal genetic diversity and relationship were investigated among eight morphologically identified native Ethiopian horse populations using polymorphisms in 46 mtDNA D-loop sequences (454 base pairs). The horse populations identified were Abyssinian, Bale, Borana, Horro, Kafa, Kundido feral horses, Ogaden and Selale. Mitochondrial DNA D-loop sequences were characterized by 15 variable sites that defined five different haplotypes. All genetic diversity estimates, including Reynolds’ linearized genetic distance, genetic differentiation (FST) and nucleotide sequence divergence (DA), revealed a low genetic differentiation in native Ethiopian horse populations. However, Kundido feral and Borana domestic horses were slightly diverged from the rest of the Ethiopian horse populations. We also tried to shed some light on the matrilineal genetic root of native Ethiopian horses from a network constructed by combining newly generated haplotypes and reference haplotypes deposited in the GenBank for Eurasian type Turkish Anatolian horses that were used as a genetic conduit between Eurasian and African horse populations. Ninety-two haplotypes were generated from the combined Ethio-Eurasian mtDNA D-loop sequences. A network reconstructed from the combined haplotypes using Median-Joining algorithm showed that haplotypes generated from native Ethiopian horses formed separate clusters. The present result encourages further investigation of the genetic origin of native African horses by retrieving additional mtDNA sequences deposited in the GenBank for African and Eurasian type horses.


2000 ◽  
Vol 23 (1) ◽  
pp. 71-78 ◽  
Author(s):  
Vera Margarete Scarpassa ◽  
Silvia Geurgas ◽  
Ana Maria L. Azeredo-Espin ◽  
Wanderli Pedro Tadei

In the present study, we have examined the variability in Anopheles nuneztovari mitochondrial DNA of three populations from the Brazilian Amazon and one from western Colombia (Sitronela), using four restriction endonucleases (BclI, ClaI, HindIII, SstI). The haplotype diversity (h) was slightly elevated in all populations (0.5000 to 0.6765), whereas the nucleotide diversity (pi) was lower in the Sitronela population (0.0029) and higher in populations from the Brazilian Amazon (0.0056 to 0.0098). The degree of sequence divergence (delta) estimated within the Brazilian Amazon and that in Sitronela (0.0329 to 0.0371) suggests that these geographic populations of A. nuneztovari may eventually constitute separate species. The low sequence divergence values among the three Brazilian Amazon populations (0.0012 to 0.0031) indicate that these populations are genetically similar. These results are consistent with those recently reported for allozymes of these same populations.


Biologia ◽  
2008 ◽  
Vol 63 (6) ◽  
Author(s):  
Elsa Froufe ◽  
Pedro Sousa ◽  
Paulo Alves ◽  
David Harris

AbstractThe large-clawed scorpion, Scorpio maurus, is a medically important scorpion and yet nothing is known regarding genetic diversity within this species. As a preliminary analysis we determined variation within the cytochrome oxidase 1 (CO1) mitochondrial gene from specimens from Morocco. High levels of genetic diversity were found that presented some geographical coherence. Of the two identified subspecies from Morocco, S. maurus birulai and S. maurus fuliginosus, the latter included genetically distinct lineages (8.0% uncorrected sequence divergence), indicating a detailed morphological and molecular revision is needed for this species.


1990 ◽  
Vol 87 (5) ◽  
pp. 1772-1776 ◽  
Author(s):  
R. K. Wayne ◽  
A. Meyer ◽  
N. Lehman ◽  
B. Van Valkenburgh ◽  
P. W. Kat ◽  
...  

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