Preparation of poly (A)-binding proteins from endoplasmic reticulum-containing subfractions of RAT liver cells and their use in mRNA purification

1977 ◽  
Vol 15 (1) ◽  
pp. 67-71 ◽  
Author(s):  
Bengt Axelsson ◽  
Rolf Ohlsson ◽  
Adam Deutsch ◽  
Bengt Jergil
Keyword(s):  
Endoplasmic Reticulum ◽  
Rat Liver ◽  
Binding Proteins ◽  
Liver Cells ◽  
Mrna Purification ◽  
Rat Liver Cells

scholarly journals Release of calcium from the endoplasmic reticulum by bile acids in rat liver cells.

1988 ◽  
Vol 263 (5) ◽  
pp. 2299-2303 ◽  
Author(s):  
L Combettes ◽  
M Dumont ◽  
B Berthon ◽  
S Erlinger ◽  
M Claret
Keyword(s):  
Endoplasmic Reticulum ◽  
Bile Acids ◽  
Rat Liver ◽  
Liver Cells ◽  
Rat Liver Cells

scholarly journals Effects of taurolithocholate, a Ca2+-mobilizing agent, on cell Ca2+ in rat hepatocytes, human platelets and neuroblastoma NG108-15 cell line

1991 ◽  
Vol 273 (1) ◽  
pp. 153-160 ◽  
Author(s):  
J F Coquil ◽  
B Berthon ◽  
N Chomiki ◽  
L Combettes ◽  
P Jourdon ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Bile Acid ◽  
Cell Line ◽  
Rat Liver ◽  
Neuronal Cell ◽  
Rat Hepatocytes ◽  
Cell Types ◽  
Liver Cells ◽  
Human Platelets ◽  
Rat Liver Cells

The monohydroxy bile acid taurolithocholate permeabilizes the endoplasmic reticulum to Ca2+ in rat liver cells. To assess whether this action on the endoplasmic reticulum was restricted to this tissue, the effects of bile acid were investigated in two cell types quite unrelated to rat hepatocyte, namely human platelets and neuronal NG108-15 cell line. The results showed that taurolithocholate (3-100 microM) had no effect on free cytosolic [Ca2+] in human platelets and NG108-15 cells. whereas it increased it from 180 to 520 nM in rat hepatocytes. In contrast, in cells permeabilized by saponin, taurolithocholate initiated a profound release of the stored Ca2+ from the internal Ca2+ pools in the three cell types. The bile acid released 90% of the Ca2+ pools, with rate constants of about 5 min-1 and half-maximal effects at 15-30 microM. The results also showed that, in contrast with liver cells, which displayed an influx of [14C]taurolithocholate of 2 nmol/min per mg, human platelets and the neuronal cell line appeared to be resistant to [14C]taurolithocholate uptake. The influx measured in these latter cells was about 100-fold lower than in rat liver cells. Taken together, these data suggest that human platelets and NG108-15 cells do not possess the transport system for concentrating monohydroxy bile acids into cells. However, they show that human platelets and neuronal NG108-15 possess, in common with liver cells, the intracellular system responsible for taurolithocholate-mediated Ca2+ release from internal stores.

scholarly journals A METHOD FOR THE QUANTITATIVE ESTIMATION OF CYTOPLASMIC STRUCTURES

1962 ◽  
Vol 15 (3) ◽  
pp. 481-487 ◽  
Author(s):  
Alden V. Loud
Keyword(s):  
Endoplasmic Reticulum ◽  
Rat Liver ◽  
Electron Micrographs ◽  
Quantitative Estimation ◽  
Liver Cells ◽  
Sampling Procedure ◽  
Rat Liver Cells ◽  
Normal Rat ◽  
Cytoplasmic Structures

A sampling procedure and calculations are described by which electron micrographs of cytoplasmic structures may be quantitatively analyzed. The relative areas occupied by formed bodies and by the "membrane space," the remainder of the cytoplasm, are evaluated. A method for making a measurement of the quantity of endoplasmic reticulum or other membrane profiles is described. The technic and results are illustrated with normal rat liver cells.

The Cytological Basis for Cortisone Action on Rat Liver Cells

1967 ◽  
Vol 25 ◽  
pp. 18-19
Author(s):  
Jonathan E. Friedberg ◽  
Robert R. Cardell
Keyword(s):  
Endoplasmic Reticulum ◽  
Electron Microscope ◽  
Rat Liver ◽  
Saline Solution ◽  
Smooth Endoplasmic Reticulum ◽  
Liver Cells ◽  
Free Access ◽  
Normal Hepatocyte ◽  
Rat Liver Cells ◽  
Cortisone Injection

The objective of this investigation is to provide morphological information on the action of a hormone. We have chosen to study the action of cortisone on the rat liver because it is established that this steroid has a stimulatory effect on the synthesis of the gluconeogenic enzymes. The glucocorticoids (including cortisone) are secreted by the adrenal cortex; hence, adrenalectomy renders the animal deficient in these hormones and impairs carbohydrate metabolism in the hepatocyte by reducing gluconeogenesis. Cortisone injection into an adrenalectomized rat increases the capacity of this cell to produce glucose from non-carbohydrate precursors. We have studied with the electron microscope liver cells from normal, adrenalectomized, and adrenalectomized, cortisone-treated rats. All rats were allowed free access to saline solution and were fasted for 15 hours before sacrifice.The liver cell of the adrenalectomized rat (Fig. 1) presents several striking alterations as compared with the normal hepatocyte. A conspicuous difference is the almost complete absence of particulate glycogen after the animal has been fasted for 15 hours. More remarkable is the very sparse development of the smooth endoplasmic reticulum (SER) in these glycogen-depleted cells.

scholarly journals Retinoids, retinoid-binding proteins, and retinyl palmitate hydrolase distributions in different types of rat liver cells.

1985 ◽  
Vol 26 (10) ◽  
pp. 1241-1251 ◽  
Author(s):  
W S Blaner ◽  
H F Hendriks ◽  
A Brouwer ◽  
A M de Leeuw ◽  
D L Knook ◽  
...  
Keyword(s):  
Rat Liver ◽  
Binding Proteins ◽  
Liver Cells ◽  
Retinyl Palmitate ◽  
Different Types ◽  
Rat Liver Cells ◽  
Retinoid Binding Proteins

scholarly journals Calmodulin-binding proteins in the nuclei of quiescent and proliferatively activated rat liver cells.

1990 ◽  
Vol 265 (30) ◽  
pp. 18595-18600
Author(s):  
O Bachs ◽  
L Lanini ◽  
J Serratosa ◽  
M J Coll ◽  
R Bastos ◽  
...  
Keyword(s):  
Rat Liver ◽  
Binding Proteins ◽  
Liver Cells ◽  
Calmodulin Binding ◽  
Rat Liver Cells

scholarly journals CHANGES IN FUNCTION AND STRUCTURE OF THE ENDOPLASMIC RETICULUM OF RAT LIVER CELLS AFTER ADMINISTRATION OF CYSTEINE

1962 ◽  
Vol 12 (1) ◽  
pp. 177-180 ◽  
Author(s):  
P. Emmelot ◽  
I. J. Mizrahi ◽  
R. Naccarato ◽  
E. L. Benedetti
Keyword(s):  
Endoplasmic Reticulum ◽  
Rat Liver ◽  
Liver Cells ◽  
Rat Liver Cells
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