Comparative sequence analysis of the 5′ noncoding region of classical swine fever virus strains from Europe, Asia, and America

1996 ◽  
Vol 141 (3-4) ◽  
pp. 771-777 ◽  
Author(s):  
T. Stadejek ◽  
J. Warg ◽  
J. F. Ridpath
2015 ◽  
Vol 43 (1) ◽  
pp. 22-30
Author(s):  
Mehak Bajwa ◽  
Ramneek Verma ◽  
Dipak Deka ◽  
Gagandeep Singh Dhol ◽  
Nagendra Nath Barman

2004 ◽  
Vol 38 (2) ◽  
pp. 289-297 ◽  
Author(s):  
Ming Xiao ◽  
Wenwei Lu ◽  
Jun Chen ◽  
Yujing Wang ◽  
Yamei Zhen ◽  
...  

2005 ◽  
Vol 12 (7) ◽  
pp. 877-881 ◽  
Author(s):  
Min Lin ◽  
Erin Trottier ◽  
Maria Mallory

ABSTRACT The antigenic region (residues 109 to 160) of classical swine fever virus (CSFV) protein Erns and the N-terminal antigenic region (residues 1 to 136) of protein E2 were constructed in the form of a fused, chimeric protein, C21ErnsE2, for use as an enzyme-linked immunosorbent assay (ELISA) antigen for the serodiagnosis of CSFV infection. Tested with 238 negative-field (CSFV-free) sera from Canadian sources, the specificity of the ELISA was determined to be 93.7%. All 20 sera from experimentally infected pigs representing a variety of animals, virus strains, and days postinfection (dpi; range, 7 to 210) were detected as positive (100%). In contrast, an ELISA based on an Erns fragment (Erns aa 109-160) or an E2 fragment (E2aa 1-221) identified only 18 (90%) of 20 sera from infected pigs as positive, missing two targets collected at 7 dpi. These data suggest that use of the chimeric antigen C21ErnsE2 would improve serodiagnostic sensitivity and allow for the detection of CSFV infection as early as 7 dpi.


Virus Genes ◽  
1993 ◽  
Vol 7 (1) ◽  
pp. 83-88 ◽  
Author(s):  
Kazufumi Hiramatsu ◽  
Kazuaki Mannen ◽  
Kumato Mifune ◽  
Akira Nishizono ◽  
Yoshiko Takita-Sonoda

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