Glycoprotein E2 of bovine viral diarrhea virus expressed in insect cells provides calves limited protection from systemic infection and disease

1996 ◽  
Vol 141 (8) ◽  
pp. 1463-1477 ◽  
Author(s):  
S. R. Bolin ◽  
J. F. Ridpath
Keyword(s):  
Bovine Viral Diarrhea Virus ◽  
Insect Cells ◽  
Systemic Infection ◽  
Bovine Viral Diarrhea ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Glycoprotein E2 ◽  
Viral Diarrhea Virus

scholarly journals Expression of Bovine Viral Diarrhea Virus Glycoprotein E2 as a Soluble Secreted Form in a Mammalian Cell Line

2006 ◽  
Vol 13 (6) ◽  
pp. 698-701 ◽  
Author(s):  
Gaetano Donofrio ◽  
Ezio Bottarelli ◽  
Cavirani Sandro ◽  
Cesidio Filippo Flammini
Keyword(s):  
Cell Line ◽  
Bovine Viral Diarrhea Virus ◽  
Natural Infection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bovine Viral Diarrhea ◽  
Type I ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Glycoprotein E2 ◽  
Viral Diarrhea Virus

ABSTRACT Bovine viral diarrhea virus (BVDV) membrane-anchored type I glycoprotein E2 is an ∼53-kDa immunodominant glycoprotein inducing the production of neutralizing antibodies in the animal host after natural infection or following immunization with live or killed vaccines. The E2 coding region lacking the transmembrane domain was constructed in a soluble secreted form (secE2) and expressed in the medium of a transiently transfected human cell line. The crude conditioned medium containing secE2 can be potentially employed to develop an enzyme-linked immunosorbent assay antigen for the diagnosis of BVDV infection or for vaccine purposes.

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