Studies on the role of recombinant human erythropoietin in the growth regulation of human nonhematopoietic tumor cells in vitro

1991 ◽  
Vol 63 (1) ◽  
pp. 5-8 ◽  
Author(s):  
W. E. Berdel ◽  
D. Oberberg ◽  
B. Reufi ◽  
E. Thiel
Keyword(s):  
Tumor Cells ◽  
Recombinant Human Erythropoietin ◽  
Growth Regulation ◽  
Human Erythropoietin

scholarly journals NLRP7 Promotes Choriocarcinoma Growth and Progression through the Establishment of an Immunosuppressive Microenvironment

Cancers ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2999
Author(s):  
Deborah Reynaud ◽  
Roland Abi Nahed ◽  
Nicolas Lemaitre ◽  
Pierre-Adrien Bolze ◽  
Wael Traboulsi ◽  
...  
Keyword(s):  
Immune Response ◽  
Tumor Cells ◽  
Major Gene ◽  
Critical Role ◽  
Orthotopic Model ◽  
Independent Manner ◽  
Maternal Immune Response ◽  
The Impact

The inflammatory gene NLRP7 is the major gene responsible for recurrent complete hydatidiform moles (CHM), an abnormal pregnancy that can develop into gestational choriocarcinoma (CC). However, the role of NLRP7 in the development and immune tolerance of CC has not been investigated. Three approaches were employed to define the role of NLRP7 in CC development: (i) a clinical study that analyzed human placenta and sera collected from women with normal pregnancies, CHM or CC; (ii) an in vitro study that investigated the impact of NLRP7 knockdown on tumor growth and organization; and (iii) an in vivo study that used two CC mouse models, including an orthotopic model. NLRP7 and circulating inflammatory cytokines were upregulated in tumor cells and in CHM and CC. In tumor cells, NLRP7 functions in an inflammasome-independent manner and promoted their proliferation and 3D organization. Gravid mice placentas injected with CC cells invalidated for NLRP7, exhibited higher maternal immune response, developed smaller tumors, and displayed less metastases. Our data characterized the critical role of NLRP7 in CC and provided evidence of its contribution to the development of an immunosuppressive maternal microenvironment that not only downregulates the maternal immune response but also fosters the growth and progression of CC.

scholarly journals Immunological and Nonimmunological Effects of Indoleamine 2,3-Dioxygenase on Breast Tumor Growth and Spontaneous Metastasis Formation

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Vera Levina ◽  
Yunyun Su ◽  
Elieser Gorelik
Keyword(s):  
Tumor Cells ◽  
Breast Tumor ◽  
Growth Regulation ◽  
Breast Cancer Cell Lines ◽  
Tumor Escape ◽  
Metastasis Formation ◽  
Spontaneous Metastasis ◽  
Immunodeficient Mice ◽  
Indoleamine 2,3 Dioxygenase

The role of the tryptophan-catabolizing enzyme, indoleamine 2,3-dioxygenase (IDO1), in tumor escape and metastasis formation was analyzed using two pairs ofIdo1+andIdo1−murine breast cancer cell lines.Ido1expression in 4T1 cells was knocked down by shRNA, andIdo1expression in NT-5 cells was upregulated by stable transfection. Growth ofIdo1−tumors and spontaneous metastasis formation were inhibited in immunocompetent mice. A higher level of cytotoxic T lymphocytes was generated by spleen cells from mice bearingIdo1−tumors thanIdo1+tumors. Tumor and metastatic growth was enhanced in immunodeficient mice, confirming an intensified immune response in the absence ofIdo1expression. However,Ido1+tumors grow faster thanIdo1−tumors in immunodeficient SCID/beige mice (lacking T, B, and NK cells) suggesting that someIdo1-controlled nonimmunological mechanisms may be involved in tumor cell growth regulation.In vitroexperiments demonstrated that downregulation ofIdo1in tumor cells was associated with decreased cell proliferation, increased apoptosis, and changed expression of cell cycle regulatory genes, whereas upregulation ofIdo1in the cells had the opposite effects. Taken together, our findings indicate thatIdo1expression could exert immunological and nonimmunological effects in murine breast tumor cells.

scholarly journals Changes in the kinetics and biopotency of luteinizing hormone in hemodialyzed men during treatment with recombinant human erythropoietin.

1994 ◽  
Vol 5 (5) ◽  
pp. 1208-1215
Author(s):  
F Schaefer ◽  
B van Kaick ◽  
J D Veldhuis ◽  
G Stein ◽  
K Schärer ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Half Life ◽  
Recombinant Human Erythropoietin ◽  
Cell Mass ◽  
Elimination Kinetics ◽  
Deconvolution Analysis ◽  
Human Erythropoietin ◽  
Change In Mean ◽  
Plasma Half Life

To investigate the effect of recombinant human erythropoietin (rh-EPO) on the hypothalamo-pituitary-gonadal axis in end-stage renal failure, plasma luteinizing hormone (LH) concentration release was assessed by frequent blood sampling (every 10 min), both during an 8-h baseline period and after stimulation with an iv bolus of gonadotropin-releasing hormone (GnRH). Seven adult hemodialyzed men were studied before and after partial correction of anemia by rh-EPO treatment. LH was determined by an in vitro Leydig cell bioassay (bio-LH) and a highly sensitive immunoradiometric assay. Pulsatile bio-LH secretion and clearance characteristics were assessed by multiple-parameter deconvolution analysis. Although the rh-EPO treatment did not lead to a change in average concentrations of plasma bio-LH, the mass of hormone released per secretory burst more than doubled, and the estimated bio-LH production rate increased from 8.8 +/- 2.3 to 15.6 +/- 5.2 IU/L per hour (P = 0.05). The lack of change in mean plasma bio-LH is explained by a simultaneous decrease in plasma half-life from 106 +/- 27 to 67 +/- 19 min (P < 0.02). The decrease in the plasma half-life of bio-LH was closely associated with the rise in hematocrit, suggesting an effect of the increased red blood cell mass on LH distribution space and elimination kinetics. As a consequence of the changes in hormone kinetics, the incremental amplitudes of the plasma concentration pulses of bio-LH increased from 112 to 121% of nadir levels (P < 0.05), resulting in a more distinctly pulsatile pattern of hormone signals.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals The role of carbohydrate in recombinant human erythropoietin

1990 ◽  
Vol 188 (2) ◽  
pp. 405-411 ◽  
Author(s):  
Eisuke TSUDA ◽  
Gosei KAWANISHI ◽  
Masatsugu UEDA ◽  
Seiji MASUDA ◽  
Ryuzo SASAKI
Keyword(s):  
Recombinant Human Erythropoietin ◽  
Human Erythropoietin

scholarly journals Treatment of the anemia of rheumatoid arthritis with recombinant human erythropoietin: Clinical and in vitro studies

1989 ◽  
Vol 32 (5) ◽  
pp. 638-642 ◽  
Author(s):  
Robert T. Means ◽  
Nancy J. Olsen ◽  
Sanford B. Krantz ◽  
Emmanuel N. Dessypris ◽  
Stanley E. Graber ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Recombinant Human Erythropoietin ◽  
In Vitro Studies ◽  
Human Erythropoietin
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