Granulocyte colony-stimulating factor (G-CSF) serum levels in surgical intensive care patients

Infection ◽  
1997 ◽  
Vol 25 (4) ◽  
pp. 213-216 ◽  
Author(s):  
W. Gross-Weege ◽  
K. Dumon ◽  
A. Dahmen ◽  
E. M. Schneider ◽  
H. D. Röher
Keyword(s):  
Intensive Care ◽  
Serum Levels ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Surgical Intensive Care ◽  
Intensive Care Patients ◽  
Stimulating Factor ◽  
Factor G

scholarly journals Can granulocyte colony stimulating factor (G-CSF) ameliorate acetaminophen-induced hepatotoxicity?

2021 ◽  
pp. 096032712110085
Author(s):  
EA Ahmed ◽  
AM Abd-Eldayem ◽  
E Ahmed
Keyword(s):  
Liver Damage ◽  
Liver Toxicity ◽  
Serum Levels ◽  
Hepatic Tissue ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Oxidative Markers ◽  
Stimulating Factor ◽  
New Strategies ◽  
Factor G

Acetaminophen (APAP) is often used as an antipyretic and analgesic agent. Overdose hepatotoxicity, which often results in liver cell failure and liver transplantation, is a severe complication of APAP usage. To save the liver and save lives from acute liver damage caused by APAP, the search for new strategies for liver defense is important. Wistar rats have been used for the induction of APAP hepatotoxicity. Elevated levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) were evaluated for liver toxicity. In addition, the levels of hepatic tissue oxidative markers such as malondialdehyde (MDA), nitric oxide (NO) increased while glutathione (GSH) was depleted and catalase (CAT) activity was curtailed. The biochemical findings were consistent with the changes in histology that suggested liver damage and inflammation. Treated rats with N-acetylcysteine (N-AC) and granulocyte colony stimulating factor (G-CSF) showed a decrease in serum levels of ALT, AST and LDH, while the level of ALP in the G-CSF group was still high. After administration of APAP, treatment with N-AC or G-CSF substantially reduced the level of MDA and NO while maintaining the GSH content and CAT activity. Treatment with N-AC and G-CSF after administration of APAP has also attenuated inflammation and hepatocytes necrosis. The results of this study showed that G-CSF could be viewed as an alternative hepatoprotective agent against APAP-induced acute liver injury compared to N-AC.

Functional Activity of Granulocytes Primed In Vivo with Glycosylated Granulocyte Colony-Stimulating Factor (G-CSF) Is Superior To Priming with Non-Glycosylated G-CSF.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 3865-3865
Author(s):  
Daniel Ribeiro ◽  
Stephanie Laufs ◽  
Marlon R. Veldwijk ◽  
Jens W. Zeller ◽  
Anthony D. Ho ◽  
...  
Keyword(s):  
Oxidative Burst ◽  
Functional Activity ◽  
Antigen Expression ◽  
Serum Levels ◽  
Granulocyte Colony Stimulating Factor ◽  
Superoxide Anions ◽  
Colony Stimulating Factor ◽  
Chemiluminescence Assay ◽  
Stimulating Factor ◽  
Factor G

Abstract Recombinant granulocyte colony-stimulating factor (G-CSF) is widely used in the treatment of chemotherapy-induced neutropenia as well as in mobilization of peripheral blood stem cells in context with autologous bone marrow transplantation. Beside recombinant non-glycosylated G-CSF expressed in E.coli (Filgrastim) and glycosylated G-CSF expressed in CHO-cells (Lenograstim), pegylated filgrastim (Pegfilgrastim) has been introduced for single-administration into clinical use. Previous studies suggest a different functional activity of glycosylated vs. non-glycosylated G-CSF. Here we study the effects of these different G-CSF on chemotaxis, oxidative burst and antigen expression of granulocytes including and correlated the results with G-CSF serum levels. Granulocytes were obtained from 27 patients with hematological malignancies before and after the administration of one of the three G-CSFs (10 Lenograstim, 9 Filgrastim, 8 Pegfilgrastim) and isolated using a polymorphprep density gradient. Chemotactic properties were assessed using a Boyden chamber assay in combination with an under agarose assay, both using fMLP as chemotactic stimulus. Release of superoxide anions served as measure of the oxidative burst after stimulation with PMA using a chemiluminescence assay. The viability and surface antigen expression were assessed by FACS. In addition G-CSF serum levels were determined by ELISA. Patients receiving Filgrastim showed a significantly impaired chemotaxis contrary to patients receiving Lenograstim (p<0.05), in the Pegfilgrastim group patients showed a strong, yet not significant reduction when compared to Lenograstim. No significant effects could be shown in production of superoxide anions in a chemiluminescence assay. In all three groups, FACS analysis showed a decrease in expression of CD10, CD11b, CD18 and CD62L and a significant increase of the LPS-receptor CD14 as well as an increased expression of the IgG receptor FcγRI (CD64) following G-CSF treatment. The increase of CD64 in the Pegfilgrastim group was significantly stronger compared to the other groups. Moreover, we observed a significantly stronger increase of CD14 in patients receiving Lenograstim when compared to the Filgrastim group. We did not observe significant differences in G-CSF serum levels between the three groups, the highest mean serum concentrations were found in the Pegfilgrastim group. Our data argue for an improved functionality of granulocytes primed with glycosylated G-CSF.

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