scholarly journals Evaluation by polymerase chain reaction of cytomegalovirus reactivation in intensive care patients under mechanical ventilation

1996 ◽  
Vol 22 (11) ◽  
pp. 1244-1249 ◽  
Author(s):  
F. Stéphan ◽  
F. Clergue ◽  
D. Méhari ◽  
S. Ricci ◽  
A. Fajac ◽  
...  
Keyword(s):  
Mechanical Ventilation ◽  
Polymerase Chain Reaction ◽  
Intensive Care ◽  
Chain Reaction ◽  
Intensive Care Patients ◽  
Cytomegalovirus Reactivation ◽  
Polymerase Chain

scholarly journals Coronavirus Disease 2019 in a Hemodialysis Patient

2020 ◽  
Vol 49 (6) ◽  
pp. 761-764 ◽  
Author(s):  
Hai Yuan ◽  
E. Guo ◽  
Zhao Gao ◽  
Fengqi Hu ◽  
Li Lu
Keyword(s):  
Mechanical Ventilation ◽  
Polymerase Chain Reaction ◽  
Hemodialysis Patient ◽  
Multiple Organ ◽  
Lung Imaging ◽  
Maintenance Hemodialysis ◽  
Multiple Organ Dysfunction ◽  
Chain Reaction ◽  
Pharyngeal Swab ◽  
Polymerase Chain

There has been a global outbreak of the coronavirus disease 2019 (COVID-19) since December 2019. Here, we describe the case of a 49-year-old male undergoing maintenance hemodialysis (HD) who got infected with COVID-19 and our experience in performing HD for him. The patient’s symptoms and lung imaging changes were atypical. However, his lymphocyte range decreased upon admission and the polymerase chain reaction of the pharyngeal swab for the ­COVID-19 nucleic acid was positive. The patient developed respiratory failure and required mechanical ventilation 8 days after admission. In the end, he died from multiple organ dysfunction syndrome. The difficulties in diagnosis, infection control, and treatment of COVID-19 in maintenance HD patients are discussed in this report.

scholarly journals Molecular Species Identification of Candida from Blood Samples of Intensive Care Unit Patients by Polymerase Chain Reaction – Restricted Fragment Length Polymorphism

2012 ◽  
Vol 4 (01) ◽  
pp. 001-004 ◽  
Author(s):  
Ramraj Vijayakumar ◽  
Sidhartha Giri ◽  
Anupma Jyoti Kindo
Keyword(s):  
Intensive Care Unit ◽  
Polymerase Chain Reaction ◽  
Intensive Care ◽  
Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Chain Reaction ◽  
Candida Spp ◽  
Blood Samples ◽  
Pcr Rflp ◽  
Polymerase Chain

ABSTRACT Introduction: Candida spp is an emerging cause of blood stream infections worldwide. Delay in speciation of Candida isolates by conventional methods and resistance to antifungal drugs (especially fluconazole, amphotericin B, etc.) in various Candida species are some of the factors responsible for the increase in morbidity and mortality due to candidemia. So, the rapid detection and identification of Candida isolates from blood is very important for the proper management of patients having candidemia. Materials and Methods: In this study, we have used polymerase chain reaction (PCR) - restriction fragment length polymorphism (RFLP) as a method for the speciation of Candida isolates from blood samples of intensive care unit (ICU) patients. PCR was used to amplify the ITS-1 and ITS-2 regions of Candida spp using universal primers ITS-1 and ITS-4. The amplified product was digested using Msp I restriction enzyme by RFLP. Results and Discussion: The method PCR-RFLP helped in identifying five medically important Candida spp (C. tropicalis, C. albicans, C. parapsilosis, C. krusei and C. glabrata) from blood. This method is rapid, reliable, easy and cost-effective and can be used in routine laboratory diagnostics for the rapid identification of Candida isolates from blood. Conclusion: PCR-RFLP is an easy, rapid and highly valuable tool which can be used in routine diagnostic laboratories to speciate Candida isolates obtained from blood. This rapid method of speciation will help clinicians to decide on empirical therapy in candidemia cases before antifungal susceptibility results are available.

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