Direct in vivo biotinylation of erythrocytes as an assay for red cell survival studies

1991 ◽  
Vol 63 (4) ◽  
pp. 214-217 ◽  
Author(s):  
G. Hoffmann-Fezer ◽  
H. Maschke ◽  
H. J. Zeitler ◽  
P. Gais ◽  
W. Heger ◽  
...  
Keyword(s):  
Cell Survival ◽  
Red Cell ◽  
Red Cell Survival ◽  
Survival Studies

Splenomegaly with Hypersplenism in Sickle Cell Anemia Treated by Radiation—Case Report

PEDIATRICS ◽  
1971 ◽  
Vol 48 (3) ◽  
pp. 457-458
Author(s):  
Annemarie Sommer ◽  
Stella B. Kontras
Keyword(s):  
Cell Survival ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Marked Improvement ◽  
Costal Margin ◽  
Red Cell ◽  
Older Children ◽  
Before And After ◽  
Red Cell Survival ◽  
Survival Studies

The incidence of splenomegaly in sickle cell anemia (defined as a spleen easily palpated below the costal margin in quiet respiration) appears to be around 10% after 10 years of age. Persistent splenomegaly in older children is rare and frequently is associated with hypersplenism. Splenectomy has been the treatment of choice in several reported cases based on red cell survival studies before and after splenectomy.1-3 Removal of the spleen has been found to be associated with marked improvement of previously very shortened red cell survival. We want to report the case of a 12-year-old boy with sickle cell anemia, splenomegaly, and sickle cell heart disease who was treated by radiation therapy for his enlarged spleen because of hypersplenism.

scholarly journals Reticulocyte Survival in Sickle Cell Anemia: Effect of Cyanate

Blood ◽  
1972 ◽  
Vol 40 (5) ◽  
pp. 733-739 ◽  
Author(s):  
Blanche P. Alter ◽  
Yuet Wai Kan ◽  
David G. Nathan
Keyword(s):  
Cell Survival ◽  
Sickle Cell ◽  
Fetal Hemoglobin ◽  
Red Cells ◽  
Red Cell ◽  
Hemoglobin Molecule ◽  
Red Cell Survival ◽  
And Control

Abstract Cyanate prevents sickling in vitro and apparently prolongs the survival of 51Cr-tagged sickle erythrocytes in vivo. Cautious interpretation is required because the effects of cyanate on 51Cr binding to sickle and fetal hemoglobin-containing red cells are unknown, and comparison of the effect of cyanate on sickle red cell survival to control red cell survival must be performed sequentially. We have studied the survival of sickle reticulocytes utilizing radioactive amino acids that are incorporated into hemoglobin. Two informed adult patients with sickle cell disease were studied. In each study, two 50-ml samples of blood were incubated separately with 14C- and 3H-leucine for 2 hr, after which 50 mM cyanate was added to one aliquot for 1 hr. The cells were then washed and reinfused. Frequent venous samples were obtained, and the specific activities of 14C and 3H in the hemoglobin were followed. The t ½ of the carbamylated cells was tripled, but remained below normal. This method provides a generally useful measurement of the influence of drugs bound to red cells on reticulocyte lifespan. The labels are incorporated into the hemoglobin molecule of the reticulocyte, and simultaneous comparison of the survivals of the same cohort of drug-treated and control cells is achieved.

Comparison of in vivo red cell survival of donations collected by Haemonetics MCS versus conventional collection

1997 ◽  
Vol 7 (1) ◽  
pp. 25-28 ◽  
Author(s):  
F. Regan ◽  
P. Teesdale ◽  
S. Garner ◽  
T. Callaghan ◽  
M. Brennan ◽  
...  
Keyword(s):  
Cell Survival ◽  
Red Cell ◽  
Red Cell Survival

In vivo studies of the long-term 51Cr red cell survival of serologically incompatible red cell units

Transfusion ◽  
1985 ◽  
Vol 25 (1) ◽  
pp. 34-38 ◽  
Author(s):  
ML Baldwin ◽  
PM Ness ◽  
C Barrasso ◽  
TS Kickler ◽  
H Drew ◽  
...  
Keyword(s):  
Cell Survival ◽  
In Vivo Studies ◽  
Red Cell ◽  
Red Cell Survival

scholarly journals New approaches to the transfusion management of thalassemia

Blood ◽  
1980 ◽  
Vol 55 (1) ◽  
pp. 55-60 ◽  
Author(s):  
RD Propper ◽  
LN Button ◽  
DG Nathan
Keyword(s):  
Cell Survival ◽  
Blood Volume ◽  
Whole Blood ◽  
Transfusion Requirement ◽  
Thalassemia Major ◽  
Iron Accumulation ◽  
Red Cell ◽  
Plasma Iron ◽  
Red Cell Survival

Recent advances in the treatment of patients with thalassemia major have centered around the removal of iron from individuals already overloaded due to repeated transfusions. In this report we present therapeutic maneuvers designed to decrease the rate of iron accumulation. We demonstrate that the persistent maintenance of hematocrits above 35% (“supertransfusion”) is not associated with an increased transfusion requirement because it produces a decrease in whole blood volume (21% +/- 2%). Supertransfusion is also associated with normalization or even prolongation of plasma iron turnover. In addition, we describe a method for obtaining units of blood from normal donors that contain primarily young red cells (“neocytes”). These cells have prolonged in vivo survival as measured by the interval between transfusions (30 +/- 2.5 days to 43 +/- 4.5 days) and 51Cr red cell survival (43.8 days versus 27.8 days). Supertransfusion with neocytes is effective in decreasing the rate of iron accumulation in thalassemia.

Sign in / Sign up

Export Citation Format

Share Document