Comparison of an automated enzyme immunoassay with a direct fluorescent antibody test and polymerase chain reaction for the detection ofChlamydia trachomatis in diagnostic specimens from male patients

1996 ◽  
Vol 15 (4) ◽  
pp. 336-340 ◽  
Author(s):  
C. Y. W. Tong ◽  
C. Valentine ◽  
O. P. Arya
Keyword(s):  
Polymerase Chain Reaction ◽  
Enzyme Immunoassay ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Chain Reaction ◽  
Ofchlamydia Trachomatis ◽  
Direct Fluorescent Antibody ◽  
Male Patients ◽  
Polymerase Chain ◽  
Detection Ofchlamydia Trachomatis

scholarly journals A Comparative Analysis of Polymerase Chain Reaction and Direct Fluorescent Antibody Test for Diagnosis of Genital Herpes

2017 ◽  
Vol 9 (01) ◽  
pp. 053-056 ◽  
Author(s):  
Vrushali Patwardhan ◽  
Preena Bhalla ◽  
Deepti Rawat ◽  
Vijay Kumar Garg ◽  
Kabir Sardana ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Genital Herpes ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Conventional Polymerase Chain Reaction ◽  
Genital Ulcer ◽  
Chain Reaction ◽  
Direct Fluorescent Antibody ◽  
Polymerase Chain ◽  
Simplex Virus

ABSTRACT Objective: To compare laboratory tests that can simultaneously detect and type herpes simplex virus (HSV) directly from the genital ulcer specimens in clinically suspected cases of genital herpes. Materials and Methods: A study was conducted over 10 months and 44 adult male and female patients clinically suspected with genital herpes were recruited. Genital ulcer swab specimens were subjected to glycoprotein-G gene-based conventional polymerase chain reaction (PCR) and commercially available direct fluorescent antibody (DFA) test and the results were compared. Results: PCR for HSV was positive in 82% (36/44) cases. DFA was positive in 68.2% (30/44) cases. There was 100% agreement between HSV types detected by DFA and PCR. The strength of agreement between the results was better in primary genital herpes than recurrent cases. Conclusion: PCR was found to be better in the detection of HSV in recurrent genital herpes patients. It is a better modality, especially when genital herpes clinically presents with ulcerative or crusted lesions, and is also a cheaper alternative as compared to DFA.

Prevalence of Mycoplasma genitalium in men with gonococcal urethritis

1996 ◽  
Vol 7 (6) ◽  
pp. 443-444 ◽  
Author(s):  
M Uno ◽  
T Deguchi ◽  
H Komeda ◽  
M Yasuda ◽  
M Tamaki ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Enzyme Immunoassay ◽  
Mycoplasma Genitalium ◽  
Chain Reaction ◽  
Gonococcal Urethritis ◽  
Japanese Male ◽  
Male Patients ◽  
Polymerase Chain

A significant association of Mycoplasma genitalium with non-gonococcal urethritis has been reported, but the prevalence of this mycoplasma in men with gonococcal urethritis has not been so well studied. In this study, we examined urethral swab specimens from 45 Japanese male patients with gonococcal urethritis for the presence of M. genitalium by using a polymerase chain reaction-based assay. We also sought Chlamydia trachomatis by an enzyme immunoassay Chlamydiazyme . Of the 45 specimens, 2 4.4 were positive for the mycoplasma and 12 26.7 were positive for C. trachomatis. The findings suggest that M. genitalium may be a cause not only of non-gonococcal urethritis but also of postgonococcal urethritis.

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