Light microscopical detection of antigens and lectin binding sites with gold-labelled reagents on semi-thin Lowicryl K4M sections: Usefulness of the photochemical silver reaction for signal amplification

1987 ◽  
Vol 19 (4) ◽  
pp. 235-245 ◽  
Author(s):  
D. J. Taatjes ◽  
U. Schaub ◽  
J. Roth
Keyword(s):  
Binding Sites ◽  
Signal Amplification ◽  
Lectin Binding ◽  
Lowicryl K4m ◽  
Lectin Binding Sites

scholarly journals Applications of immunocolloids in light microscopy. III. Demonstration of antigenic and lectin-binding sites in semithin resin sections.

1984 ◽  
Vol 32 (10) ◽  
pp. 1075-1083 ◽  
Author(s):  
J M Lucocq ◽  
J Roth
Keyword(s):  
Submandibular Gland ◽  
Binding Sites ◽  
Lectin Binding ◽  
Protein A ◽  
Gold Complexes ◽  
Specific Staining ◽  
Maximum Information ◽  
Nomarski Differential Interference Contrast ◽  
Lowicryl K4m ◽  
Lectin Binding Sites

Previous studies have demonstrated that antigens or lectin-binding sites can be localized in sections from paraffin-embedded tissues with protein A or lectins bound to colloidal gold or colloidal silver (Roth J: J Histochem Cytochem 30:691, 1982 and 31:547, 1983). In the present study the protein A-gold technique and lectin-gold complexes have been applied to semithin sections (0.5-1.5 micron) of Epon- or low temperature Lowicryl K4M-embedded rat pancreas, kidney and submandibular gland. The results show that an increase in resolution and, therefore, in amount of information can be obtained. The optimal mode of imaging was determined on sections without counterstaining. Bright-field illumination gives the maximum information about the staining signal, while phase-contrast and Nomarski differential interference contrast give predominantly structural and, to a lesser extent, staining information. Polarization epi- and transillumination microscopy is inferior in all aspects. The application of a battery of lectin-gold complexes to rat submandibular gland revealed a specific staining pattern for each lectin in acinar and excretory duct cells.

scholarly journals Nuclear and cytoplasmic lectin binding sites in promastigotes of Leishmania.

1991 ◽  
Vol 39 (6) ◽  
pp. 793-800 ◽  
Author(s):  
M A Vannier-Santos ◽  
E M Saraiva ◽  
W de Souza
Keyword(s):  
Sialic Acid ◽  
Binding Sites ◽  
Ricinus Communis ◽  
Lectin Binding ◽  
Gold Complex ◽  
Carbohydrate Binding ◽  
Nuclear Compartment ◽  
Ricinus Communis Agglutinin ◽  
Lowicryl K4m ◽  
Lectin Binding Sites

We demonstrated the presence of intracellular lectin binding sites in promastigotes of Leishmania mexicana amazonensis. Direct and indirect lectin-gold techniques were used on Lowicryl K4M-embedded cells. The nuclear compartment was labeled by most lectins. Nucleoli were mainly labeled by WFH (Wistaria floribunda hemagglutinin) and LFA (Limax flavus agglutinin) specific for D-galactose/N-acetyl-D-galactosamine (D-Gal/D-GalNAc) and sialic acid, respectively. Sections treated with the fetuin-gold complex without previous lectin incubation also exhibited labeled nucleoli, although less intensely, suggesting the presence not only of sialic acid but also of a sialic acid-specific endogenous carbohydrate binding molecule in Leishmania nuclei. Surprisingly, the Golgi complex was never labeled, whereas the endoplasmic reticulum was frequently labeled, especially by RCA (Ricinus communis agglutinin; D-GalNAc/D-Gal) and WGA (wheat germ agglutinin; D-GlcNAc). The kinetoplast, a DNA-containing structure located within the mitochondrion, was generally labeled towards its extremities, where previous studies have shown the presence of ribonucleoproteins. Some possible biological roles for these intracellular glycoconjugates are discussed.

Lectin-binding sites in chick limb buds

1989 ◽  
Vol 27 ◽  
pp. 82
Author(s):  
M. Narita ◽  
K. Yamashita ◽  
M. Yasuda
Keyword(s):  
Binding Sites ◽  
Lectin Binding ◽  
Limb Buds ◽  
Lectin Binding Sites

Ultrastructural demonstration of lectin binding sites in the Golgi apparatus of rat epiphyseal chondrocytes

1988 ◽  
Vol 89 (2) ◽  
pp. 177-184 ◽  
Author(s):  
A. Velasco ◽  
J. Hidalgo ◽  
M. M�ller ◽  
G. Garcia-Herdugo
Keyword(s):  
Golgi Apparatus ◽  
Binding Sites ◽  
Lectin Binding ◽  
Lectin Binding Sites

Lectin binding sites of the mouse ovary, intraovarian and ovulated ova

1984 ◽  
Vol 80 (6) ◽  
pp. 527-533 ◽  
Author(s):  
T. -C. Wu ◽  
M. -C. Lee ◽  
Y. -J. Wan ◽  
I. Damjanov
Keyword(s):  
Binding Sites ◽  
Lectin Binding ◽  
Mouse Ovary ◽  
Lectin Binding Sites
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