Patch-clamp capacitance measurements: A tool for investigating the second messengers regulating exocytosis

1989 ◽  
Vol 8 (3) ◽  
pp. 438-441
Author(s):  
Manfred Lindau
Keyword(s):  
Patch Clamp ◽  
Second Messengers ◽  
Capacitance Measurements

scholarly journals Review the Regulation of Plasma Membrane Calcium Channel in Cancer and Patch Clamp Technique

2021 ◽  
Vol 271 ◽  
pp. 04037
Author(s):  
Nanjun Chen ◽  
Qigeng Fang
Keyword(s):  
Plasma Membrane ◽  
Patch Clamp ◽  
Calcium Channels ◽  
Cancer Progression ◽  
Second Messengers ◽  
Research Progress ◽  
Patch Clamp Technique ◽  
Voltage Gated Calcium Channels ◽  
Cell Life ◽  
Cell Plasma

As one of the most versatile and universal second messengers, calcium plays an essential role in cell life. Here we briefly reviewed the research progress of how different calcium channels are located at the cell plasma membrane, including voltage-gated calcium channels (VGCCs), receptor-operated channels (ROC), and store-operated channels (ROC). These channels can regulate different cancer progression. Afterward, the patch clamp technique's development and operating principle, an important quantitative method used for ion channel investigation, are introduced in this paper.

Patch-Clamp Capacitance Measurements: New Insights into the Endocytic Uptake of Transferrin

2002 ◽  
Vol 29 (3) ◽  
pp. 459-464 ◽  
Author(s):  
Lukas Schwake ◽  
Andreas W. Henkel ◽  
Hans D. Riedel ◽  
Wolfgang Stremmel
Keyword(s):  
Patch Clamp ◽  
Capacitance Measurements

Patch-Clamp Capacitance Measurements

2012 ◽  
pp. 277-286 ◽  
Author(s):  
Takeshi Sakaba ◽  
Akaihiro Hazama ◽  
Yoshio Maruyama
Keyword(s):  
Patch Clamp ◽  
Capacitance Measurements

High-voltage electron microscopy of patch-clamped membranes

1987 ◽  
Vol 45 ◽  
pp. 582-583
Author(s):  
F. Sachs ◽  
M. J. Song
Keyword(s):  
Electron Microscopy ◽  
Cell Membrane ◽  
Patch Clamp ◽  
High Voltage Electron Microscopy ◽  
Small Patch ◽  
Cellular Electrophysiology ◽  
Voltage Electron ◽  
Electron Microscopes ◽  
Patch Technique ◽  
Membrane Patch

Cellular electrophysiology has been revolutionized by the introduction of patch clamp techniques. The patch clamp records current from a small patch of the cell membrane which has been sucked into a glass pipette. The membrane patch, a few micons in diameter, is attached to the glass by a seal which is electrically, diffusionally and mechanically tight. Because of the tight electrical seal, the noise level is low enough to record the activity of single ion channels over a time scale extending from 10μs to days. However, although the patch technique is over ten years old, the patch structure is unknown. The patch is inside a glass pipette where it has been impossible to see with standard electron microscopes. We show here that at 1 Mev the glass pipette is transparent and the membrane within can be seen with a resolution of about 30 A.

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